Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
권호 표지
DOI QR코드

DOI QR Code

Curcumin Inhibits Human Non-small Cell Lung Cancer A549 Cell Proliferation Through Regulation of Bcl-2/Bax and Cytochrome C

  • Li, Yue (Department of Medical Oncology, The Third Affiliated Hospital of Harbin Medical University) ;
  • Zhang, Shuai (Department of Medical Oncology, The Third Affiliated Hospital of Harbin Medical University) ;
  • Geng, Jian-Xiong (Department of Medical Oncology, The Third Affiliated Hospital of Harbin Medical University) ;
  • Hu, Xiao-Yang (Staff Room of Prescription, Heilongjiang University of Chinese Medicine)
  • Published : 2013.08.30
Download PDF
⟨ Previous Next ⟩

Abstract

We intended to study the mechanism of the inhibitory action of curcumin on human non-small cell lung cancer A549 cell. The cell growth was determined by CCK-8 assay, and the results indicated that curcumin inhibited the cell proliferation in a concentration dependent manner. And to further confirm the relative anti-cancer mechanism of curcumin, RT-PCR was carried out to analysis the expression of relative apoptotic proteins Bax, Bcl-2. We found that curcumin could up-regulate the expression of Bax but down-regulate the expression of Bcl-2 in A549 cells. In addition, curcumin affect the mitochondrial apoptosis pathway. These results suggested that curcumin inhibited cancer cell growth through the regulation of Bcl-2/Bax and affect the mitochondrial apoptosis pathway.

Keywords

References

  1. Aggarwal S, Ichikawa H, Takada Y, et al (2006). Curcumin (diferuloylmethane) down-regulates expression of cell proliferation and antiapoptotic and metastatic gene products through suppression of IkappaBalpha kinase and Akt activation. Mol Pharmacol, 69, 195-206.
  2. Bansal SS, Goel M, Aqil F, Vadhanam MV, Gupta RC (2011). Advanced drug delivery systems of curcumin for cancer chemoprevention. Cancer Prev Res (Phila), 4, 1158-71. https://doi.org/10.1158/1940-6207.CAPR-10-0006
  3. Howells LM, Mitra A, Manson MM (2007). Comparison of oxaliplatin- and curcumin-mediated antiproliferative effects in colorectal cell lines. Int J Cancer, 121, 175-83. https://doi.org/10.1002/ijc.22645
  4. Jaiswal AS, Marlow BP, Gupta N, Narayan S (2002). Beta-catenin-mediated transactivation and cell-cell adhesion pathways are important in curcumin (diferuylmethane)-induced growth arrest and apoptosis in colon cancer cells. Oncogene, 21, 8414-27. https://doi.org/10.1038/sj.onc.1205947
  5. Na HS, Cha MH, Oh DR, et al (2011). Protective mechanism of curcumin against Vibrio vulnificus infection. FEMS Immunol Med Microbiol, 63, 355-62. https://doi.org/10.1111/j.1574-695X.2011.00855.x
  6. National Collaborating Centre for Cancer (UK) (2011). The Diagnosis and Treatment of Lung Cancer (Update). Cardiff (UK): National Collaborating Centre for Cancer (UK).
  7. Notarbartolo M, Poma P, Perri D, et al (2005). Antitumor effects of curcumin, alone or in combination with cisplatin or doxorubicin, on human hepatic cancer cells. Analysis of their possible relationship to changes in NF-kB activation levels and in IAP gene expression. Cancer Lett, 224, 53-65. https://doi.org/10.1016/j.canlet.2004.10.051
  8. Parkin DM, Bray F, Ferlay J, Pisani P (2005). Global cancer statistics, 2002. CA Cancer J Clin, 55, 74-108. https://doi.org/10.3322/canjclin.55.2.74
  9. Pettersson F, Dalgleish AG, Bissonnette RP, Colston KW (2002). Retinoids cause apoptosis in pancreatic cancer cells via activation of RAR-gamma and altered expression of Bcl-2/Bax. Br J Cancer, 87, 555-61. https://doi.org/10.1038/sj.bjc.6600496
  10. Sato T, Hanada M, Bodrug S, et al (1994). Interactions among members of the Bcl-2 protein family analyzed with a yeast two-hybrid system. Proc Natl Acad Sci U S A, 91, 9238-42. https://doi.org/10.1073/pnas.91.20.9238
  11. Schaffer M, Schaffer PM, Zidan J, Bar Sela G (2011). Curcuma as a functional food in the control of cancer and inflammation. Curr Opin Clin Nutr Metab Care, 14, 588-97. https://doi.org/10.1097/MCO.0b013e32834bfe94
  12. Song JX, Sze SC, Ng TB, et al (2012). Anti-Parkinsonian drug discovery from herbal medicines: what have we got from neurotoxic models? J Ethnopharmacol, 139, 698-711. https://doi.org/10.1016/j.jep.2011.12.030
  13. Speciale A, Chirafisi J, Saija A, Cimino F (2011). Nutritional antioxidants and adaptive cell responses: an update. Curr Mol Med, 11, 770-89. https://doi.org/10.2174/156652411798062395
  14. Wang YJ, Niu XP, Yang L, Han Z, Ma YJ (2013). Effects of celecoxib on cycle kinetics of gastric cancer cells and protein expression of cytochrome C and caspase-9. Asian Pac J Cancer Prev, 14, 2343-7. https://doi.org/10.7314/APJCP.2013.14.4.2343
  15. Xu Y, Zhang JJ, Han J, et al (2012). Curcumin inhibits tumor proliferation induced by neutrophil elastase through the upregulation of alpha1-antitrypsin in lung cancer. Mol Oncol, 6, 405-17. https://doi.org/10.1016/j.molonc.2012.03.005
  16. Yang CL, Liu YY, Ma YG, et al (2012). Curcumin blocks small cell lung cancer cells migration, invasion, angiogenesis, cell cycle and neoplasia through Janus kinase-STAT3 signalling pathway. PLoS One, 7, e37960. https://doi.org/10.1371/journal.pone.0037960
  17. Youle RJ, Strasser A (2008). The BCL-2 protein family: opposing activities that mediate cell death. Nat Rev Mol Cell Biol, 9, 47-59. https://doi.org/10.1038/nrm2308

Cited by

  1. Co-treatment of THP-1 cells with naringenin and curcumin induces cell cycle arrest and apoptosis via numerous pathways vol.12, pp.6, 2015, https://doi.org/10.3892/mmr.2015.4480
  2. Anticancer Effects of Thymoquinone, Caffeic Acid Phenethyl Ester and Resveratrol on A549 Non-small Cell Lung Cancer Cells Exposed to Benzo(a)pyrene vol.14, pp.10, 2013, https://doi.org/10.7314/APJCP.2013.14.10.6159
  3. Curcumin Analogue A501 induces G2/M Arrest and Apoptosis in Non-small Cell Lung Cancer Cells vol.15, pp.16, 2014, https://doi.org/10.7314/APJCP.2014.15.16.6893
  4. Matrine Reduces Proliferation of Human Lung Cancer Cells by Inducing Apoptosis and Changing miRNA Expression Profiles vol.15, pp.5, 2014, https://doi.org/10.7314/APJCP.2014.15.5.2169
  5. Ubiquitination of p53 is Involved in Troglitazone Induced Apoptosis in Cervical Cancer Cells vol.15, pp.5, 2014, https://doi.org/10.7314/APJCP.2014.15.5.2313
  6. Expression of Annexin A3 in Gastric Cancer and its Correlation with Proliferation and Apoptosis vol.15, pp.7, 2014, https://doi.org/10.7314/APJCP.2014.15.7.3001
  7. Induction of MicroRNA-9 Mediates Cytotoxicity of Curcumin Against SKOV3 Ovarian Cancer Cells vol.15, pp.8, 2014, https://doi.org/10.7314/APJCP.2014.15.8.3363
  8. Rice Bran Phytic Acid Induced Apoptosis Through Regulation of Bcl-2/Bax and p53 Genes in HepG2 Human Hepatocellular Carcinoma Cells vol.15, pp.8, 2014, https://doi.org/10.7314/APJCP.2014.15.8.3731
  9. Targeting Cancer with Nano-Bullets: Curcumin, EGCG, Resveratrol and Quercetin on Flying Carpets vol.15, pp.9, 2014, https://doi.org/10.7314/APJCP.2014.15.9.3865
  10. Curcumin Induces Apoptosis in SGC-7901 Gastric Adenocarcinoma Cells via Regulation of Mitochondrial Signaling Pathways vol.15, pp.9, 2014, https://doi.org/10.7314/APJCP.2014.15.9.3987
  11. Curcumin suppresses proliferation and invasion in non-small cell lung cancer by modulation of MTA1-mediated Wnt/β-catenin pathway vol.50, pp.9, 2014, https://doi.org/10.1007/s11626-014-9779-5
  12. Biological and therapeutic activities, and anticancer properties of curcumin vol.10, pp.5, 2015, https://doi.org/10.3892/etm.2015.2749
  13. Curcumin inhibits proliferation–migration of NSCLC by steering crosstalk between a Wnt signaling pathway and an adherens junction via EGR-1 vol.11, pp.3, 2015, https://doi.org/10.1039/C4MB00336E
  14. Licochalcone C induces apoptosis via B-cell lymphoma 2 family proteins in T24 cells vol.12, pp.5, 2015, https://doi.org/10.3892/mmr.2015.4346
  15. Current Drugs and Drug Targets in Non-Small Cell Lung Cancer: Limitations and Opportunities vol.16, pp.10, 2015, https://doi.org/10.7314/APJCP.2015.16.10.4147
  16. Caspase-9 as a therapeutic target for treating cancer vol.19, pp.1, 2015, https://doi.org/10.1517/14728222.2014.961425
  17. Combination treatment with triptolide and hydroxycamptothecin synergistically enhances apoptosis in A549 lung adenocarcinoma cells through PP2A-regulated ERK, p38 MAPKs and Akt signaling pathways vol.46, pp.3, 2015, https://doi.org/10.3892/ijo.2015.2814
  18. Effects of demethoxycurcumin on the viability and apoptosis of skin cancer cells vol.16, pp.1, 2017, https://doi.org/10.3892/mmr.2017.6666
  19. Curcumin reduces mitomycin C resistance in breast cancer stem cells by regulating Bcl-2 family-mediated apoptosis vol.17, pp.1, 2017, https://doi.org/10.1186/s12935-017-0453-3
  20. Insulin Growth Factor 1 Protects Neural Stem Cells Against Apoptosis Induced by Hypoxia Through Akt/Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase (Akt/MAPK/ERK) Pathway in Hypoxia-Ishchemic Encephalopathy vol.23, pp.1643-3750, 2017, https://doi.org/10.12659/MSM.901055
  21. Cinchonine induces apoptosis of HeLa and A549 cells through targeting TRAF6 vol.36, pp.1, 2017, https://doi.org/10.1186/s13046-017-0502-8
  22. Molecular Mechanisms Underlying Curcumin-Mediated Therapeutic Effects in Type 2 Diabetes and Cancer vol.2018, pp.1942-0994, 2018, https://doi.org/10.1155/2018/9698258
  23. Calreticulin protects the HT22 hippocampal neurons from injury induced by hypoxia vol.14, pp.1, 2018, https://doi.org/10.1007/s13273-018-0010-8
  24. MiR-17 Regulates Prostate Cancer Cell Proliferation and Apoptosis Through Inhibiting JAK-STAT3 Signaling Pathway vol.33, pp.3, 2018, https://doi.org/10.1089/cbr.2017.2386
  25. Curcumin and Gastric Cancer: a Review on Mechanisms of Action pp.1941-6636, 2019, https://doi.org/10.1007/s12029-018-00186-6