The Change of Ginsenoside Composition in Ginseng Leaf and Stem Extract by the Microwave and Vinegar Process

인삼 잎 줄기 추출물의 초단파 및 식초 처리에 의한 인삼 사포닌 성분 변화

  • Kim, Shin Jung (College of Pharmacy, Sookmyung Women's University) ;
  • Kim, Ju Duck (Graduate School of Distance Learning, Department of Cosmetic & Beauty, Sookmyung Women's University) ;
  • Ko, Sung Kwon (The Department of Oriental Medical Food & Nutrition, Semyung University)
  • 김신정 (숙명여자대학교 약학대학) ;
  • 김주덕 (숙명여자대학교 원격대학원) ;
  • 고성권 (세명대학교 한방식품영양학부)
  • Received : 2013.03.22
  • Accepted : 2013.06.11
  • Published : 2013.06.30

Abstract

The purpose of this study was to develop a new preparation process of ginseng extract with the high concentration of prosapogenin, the specific component in Red ginseng. Chemical transformation from the ginseng saponin glycosides to the prosapogenin was analyzed by the HPLC. The extracts of ginseng leaf and stem were processed at the several treatment conditions of the microwave and vinegar(about 14% acidity). MGLS-20 findings show that the ginseng leaf and stem extracts that had been processed with microwave and vinegar for 20 minutes peaked in the level of ginsenoside $Rg_3$(0.906%). MGLS-25 peaked in the level of ginsenoside $Rg_5$(0.329%) in the ginseng leaf and stem extract processed with microwave and vinegar for 25 minute. And the other kinds of ginseng prosapogenin did not show a higher content.

Keywords

References

  1. Ko, SK. and Im, BO. (2009) The science of Korean ginseng, 47-52, Yakupsinmunsa, Seoul.
  2. Lee, D. C., Lee, M. O., Kim, C. Y. and Clifford, D. H. (1981) Effect of ether, ethanol and aqueous extracts of ginseng on cardiovascular function in dogs. Can. J. Comp. Med. 45: 182-187
  3. Jie, Y. H., Cammisuli, S. and Baggiolini, M. (1984) Immunomodulatory effects of Panax ginseng C.A. Meyer in the mouse. Agents Actions. 15: 386-391. https://doi.org/10.1007/BF01972376
  4. Kim, Y. C., Kim, S. R., Markelonis, G. J. and Oh, T. H. (1998) Ginsenosides Rb1 and Rg3 protect cultured rat cortical cells from glutamate-induced neurodegeneration. J. Neurosci. Res. 53: 426-432. https://doi.org/10.1002/(SICI)1097-4547(19980815)53:4<426::AID-JNR4>3.0.CO;2-8
  5. Joo, C. N., Koo, J. D., Kim, D. S. and Lee, S. J. (1977) Biochemical studies of ginseng saponins. XI. The effects of ginseng saponins on alcohol dehydrogenase. Hanguk Saenghwa Hakhoe Chi. 10: 109-120.
  6. Tahara, M., Kono, H., Mune, S. and Odashima, S. (1985) Action of ginsenosides on tumor cells. Growth inhibition and redifferentiation of neoplasia. Wakan Yaku Gakkaishi. 2: 170-171
  7. Yokozawa, T., Kobayashi, T., Oura, H. and Kawashima, Y. (1985) Studies on the mechanism of the hypoglycemic activity of ginsenoside-Rb2 in streptozotocin-diabetic rats. Chem. Pharm. Bull. 33: 869-872. https://doi.org/10.1248/cpb.33.869
  8. Park, J. D. (1996) Recent studies on the chemical constituents of Korean ginseng. Korean J. Ginseng Sci. 20: 389-415.
  9. Sanata, S., Kondo, N., Shoji, J., Tanaka, O. and Shibata, S. (1974) Studies on the saponins of ginseng. . Structure of ginseng- $R_{0}$, $Rb_{1}$, $Rb_{2}$, Rc and Rd. Chem. Pharm. Bull. 22: 421-428 https://doi.org/10.1248/cpb.22.421
  10. Kitagawa, I., Taniyama, T., Shibuya, H., Nota, T. and Yoshikawa, M. (1987) Chemical studies on crude drug processing. V. On the constituents of ginseng radix rubra (2); Comparison of the constituents of white ginseng and red ginseng prepared from the same Panax ginseng root. Yakugaku Zasshi. 107: 495-505. https://doi.org/10.1248/yakushi1947.107.7_495
  11. Keum, Y. S., Park, K. K., Lee, J. M., Chun, K. S., Park, J. H., Lee, S. K., Kwon, H. and Surh, Y. J. (2000) Antioxidant and anti-tumor promoting activities of the methanol extract of heat-processed ginseng. Cancer Lett. 150: 41-48. https://doi.org/10.1016/S0304-3835(99)00369-9
  12. Kim, S. E., Lee, Y. H., Park, J. H. and Lee, S. K. (1999) Ginsenoside- Rs3, a new diol-type ginseng saponin, selectively elevates protein levels of p53 and p21WAF1 leading to induction of apoptosis in SK-HEP-1 cells. Anticancer Res. 19: 487-491.
  13. Kim, W. Y., Kim, J. M., Han, S. B., Lee, S. K., Kim, N. D., Park, M. K., Kim, C. K. and Park, J. H. (2000) Steaming of ginseng at high temperature enhances biological activity. J. Nat. Prod. 63: 1702-1704. https://doi.org/10.1021/np990152b
  14. Bao, H. Y., Zhang, J., Yeo, S. J., Myung, C. S., Kim, H. M., Kim, J. M., Park, J. H., Cho, J. S. and Kang, J. S. (2005) Memory enhancing and neuroprotective effects of selected ginsenosides. Arch. Pharm. Res. 28: 335-342. https://doi.org/10.1007/BF02977802
  15. Jung, K. Y., Kim, D. S., Oh, S. R., Lee, I. S., Lee, J. J., Park, J. D., Kim, S. I. and Lee, H. K. (1998) Platelet activating factor antagonist activity of ginsenosides. Biol. Pharm. Bull. 21: 79-80. https://doi.org/10.1248/bpb.21.79
  16. Kwon, S. W., Han, S. B., Park, I. H., Kim, J. M., Park, M. K. and Park, J. H. (2001) Liquid chromatographic determination of less polar ginsenosides in processed ginseng. J. Chromatogr. 921: 335-339. https://doi.org/10.1016/S0021-9673(01)00869-X
  17. Hasegawa, H., Sung, J. H., Matsumiya, S. and Uchiyama, M. (1996) Main ginseng saponin metabolites formed by intestinal bacteria. Planta Med. 62: 453-457. https://doi.org/10.1055/s-2006-957938
  18. Hasegawa, H., Sung, J. H. and Benno, Y. (1997) Role of human intestinal Prevotella oris in hydrolyzing ginseng saponins. Planta Med. 63: 436-440. https://doi.org/10.1055/s-2006-957729
  19. Yahara, S., Tanaka, O. and Komori, T. (1976) Saponins of the leaves of Panax ginseng C. A. Meyer. Chem. Pharm. Bull. 24: 2204-2208. https://doi.org/10.1248/cpb.24.2204
  20. Yahara. S. Matsuura. K., Kasai. R. and Tanaka. O. (1976) Saponins of buds and flowers of Panax ginseng (I). Isolation of ginsenosides Rd, Re, and $Rg_{1}$. Chem. Pharm. Bull., 24: 3212-3213. https://doi.org/10.1248/cpb.24.3212
  21. Yahara. S., Kaji. K. and Tanaka. O. (1979) Further study on dammarane-type saponins of roots, leaves, flower buds, and fruits of Panax ginseng C. A. Meyer. Chem. Pharm. Bull., 27: 88-92. https://doi.org/10.1248/cpb.27.88
  22. Zhang. S., Yao. X., Chen, Y., Cui. C., Tezuka. Y. and Kikuchi. T. (1989) Ginsenoside La, a novel saponin from the leaves of Panax ginseng. Chem. Pharm. Bull., 37: 1966-1968. https://doi.org/10.1248/cpb.37.1966
  23. Zhang. S., Takeda. T., Zhu. T., Chen. Y., Yao. X., Tanaka, O. and Ogihara. Y. (1990) A New Minor Saponin from the Leaves of Panax ginseng. Planta Medica. 56: 298-300. https://doi.org/10.1055/s-2006-960963
  24. Kwon, S. W., Han, S. B., Park, I H., Kim, J. M., Park, M. K. and Park, J. H. (2001) Liquid chromatographic determination of less polar ginsenosides in processed ginseng. Journal of chromatography A, 921: 335-339. https://doi.org/10.1016/S0021-9673(01)00869-X
  25. Ko, S. K., Lee, K. H., Hong, J. K., Kang, S. A., Sohn, U. D., Im, B. O., Han, S. T., Yang, B. W., Chung, S. H. and Lee, B. Y. (2005) Change of Ginsenoside Composition in Ginseng Extract by Vinegar Process. Food Sci. Biotechnol. 14: 509-513
  26. Shibata, S., Tanaka, T., Ando, T., Sado, M., Tsushima, S. and Ohsawa, T. (1966) Chemial Studies on oriental plant drugs (XIV) Prtopanaxadiol, a genuine sapogenin of ginseng saponins. Chem. Pharm. Bull. 14: 595-600. https://doi.org/10.1248/cpb.14.595
  27. Jo, H. K., Sung, M. C. and Ko, S. K. (2012) The comparison of ginseng prosapogenin composition and contents in Red and Black ginseng. Kor. J. Pharmacogn. 42: 361-365.