Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Analysis of the Characterizing Compounds of Korean Mistletoes (Viscum album var. coloratum)

한국산 겨우살이의 지표성분 분석 연구(제 1 보) (기주식물과 생장지역에 따른 지표성분 변화 분석)

  • Received : 2013.03.16
  • Accepted : 2013.05.22
  • Published : 2013.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

The three biochemical constituents pointed out as the bioactive principles of anticancer activity of Korean mistletoes, Viscum album var. colorantum, epiphyting to different host trees, different region, and different growth environments were compared. The characterizing compounds were lectin, which was glycoprotein reported has anticancer activity, homoflavoyadorinin-B, which was flavonoid glycoside reported as an antioxidant and oleanolic acid, as an anticancerous triterpenoid. We established the analysis methods for three characterizing compounds and determined by HPLC spectrophotometer, respectively. The average content of the lectin in V. album was 1.75 mg/g of dried plants and those of homoflavoyadorinin-B and oleanolic acid were 3.51 and 5.37 mg/g, respectively. The variations of contents of the three characterizing compounds according to epiphytic trees and growth region were observed but not found statistical significance clearly. We came to the conclusion that the contents of bioactive constituents of V. album except homoflavoyadorinin-B are not affected by epiphyting trees or growth environments.

Keywords

References

  1. Hajto, T., Berki, T., Boldizsar, F. and Nemeth, P. (2003) Galactoside specific plant lectin, Viscum album agglutinininduces enhanced proliferation and apoptosis of murine thymocytes in vivo. Immunol. Lett. 86: 23-27. https://doi.org/10.1016/S0165-2478(02)00265-1
  2. Siegle, I., Friz, P., McClellan, M., Gutzeit, S. and Murdter, T. E. (2001) Combined cytotoxic action of Viscum album agglutinin- and anticancer agent against human A549 lung cancer cells. Anticancer Res. 21: 2687-2691.
  3. Kim, Y. K., Kim, Y. S., Choi, S. U. and Ryu, S. Y. (2004) Isolation of flavonol from Loranthus tanakae and cytotoxic effect of them on human tumer cell lines. Arch. Pharm. Res. 27(1): 44-47 https://doi.org/10.1007/BF02980044
  4. Lee, S. J., Lee, M. K., Choi, G. P., Yu, C. Y., Roh, S. K., Kim, J. D., Lee, H. Y. and Lee, J. H. (2003) Growth enhancement and cytotoxicity of Korean mistletoe fractions on human cell lines. Korean J. Medicinal Crop Sci. 11: 62-70.
  5. Karagoz, A., Onay, E., Arda, N. and Kuru, A. (2003) Anti-viral potency of mistletoe (Viscum album ssp. album) extracts against human parainfluenza virus type 2 in Vero cells. Phytother. Res. 17: 560-562. https://doi.org/10.1002/ptr.1163
  6. Fernandez, T., Cerda, Z. P., Aulicino, P., Caldas, L. E., Wagner, M., Ricco, R., Hajos, S., Gurni, A. and Alvarez, E. (2003) Immunobiological features of the galactoside lectin LLc isolated from the Argentine mistletoe Ligaria cuneifolia. J. Ethnopharmacol. 85: 81-92. https://doi.org/10.1016/S0378-8741(02)00361-6
  7. Choi, J. H., Lyu, S. Y., Lee, H. J., Jung, J., Park, W. B. and Kim, G. J. (2012) Korean mistletoe lectin regulates selfrenewal of placenta-derived mesenchymal stem cells via autophagic mechanisms. Cell Prolif. 45: 420-429. https://doi.org/10.1111/j.1365-2184.2012.00839.x
  8. Lyu, S. Y. and Park, W. B. (2008) Transport of mistletoe lectin by M cells in human intestinal follicle-associated epithelium (FAE) in vitro. Arch Pharm Res. 31: 1613-1621. https://doi.org/10.1007/s12272-001-2159-1
  9. Lyu, S. Y. and Park, W. B. (2009) Mistletoe lectin modulates intestinal epithelial cell-derived cytokines and B cell IgA secretion. Arch. Pharm. Res. 32: 443-451. https://doi.org/10.1007/s12272-009-1319-6
  10. Lyu, S. Y. and Park, W. B. (2011) Gene network analysis on the effect of Viscum album var. coloratum in T cells stimulated with anti-CD3/CD28 antibodies. Arch. Pharm. Res. 34: 1735-1749. https://doi.org/10.1007/s12272-011-1018-y
  11. Park, H. J., Hong, J. H., Kwon, H. J., Kim, Y., Lee, K. H., Kim, J. B. and Song, S. K. (2010) TLR4-mediated activation of mouse macrophages by Korean mistletoe lectin-C (KMLC). Biochem. Biophys. Res. Commun. 396: 721-725. https://doi.org/10.1016/j.bbrc.2010.04.169
  12. Lee, C. H., Kim, J. K., Kim, H. Y., Park, S. M. and Lee, S. M. (2009) Immunomodulating effects of Korean mistletoe lectin in vitro and in vivo. Int. Immunopharmacol. 9: 1555- 1561. https://doi.org/10.1016/j.intimp.2009.09.011
  13. Kim, B. K., Choi, M. J., Park, K. Y. and Cho, E. J. (2010) Protective effects of Korean mistletoe lectin on radicalinduced oxidative stress. Biol. Pharm. Bull. 33: 1152-1158. https://doi.org/10.1248/bpb.33.1152
  14. Jung, H. Y., Lee, A. N., Song, T. J,, An, H. S., Kim, Y. H., Kim, K. D., Kim, I. B., Kim, K. S., Han, B. S., Kim, C. H., Kim, K. S. and Kim, J. B. (2012) Korean mistletoe (Viscum album var. coloratum) extract improves endurance capacity in mice by stimulating mitochondrial activity. J. Med. Food. 15: 621-628. https://doi.org/10.1089/jmf.2010.1469
  15. Boneberg, E. M. and Hartung, T. (2001) Mistletoe lectinincreases tumor necrosis factor-alpha release in lipopolysaccharide- stimulated whole blood via inhibition of interleukin- 10 production. J. Pharmacol. Exp. Ther. 298: 996-1000.
  16. Tabiasco, J., Pont, F., Forrnie, J. J. and Vercellone, A. (2002) Mistletoe viscotoxins increase natural killer cell-mediated cytotoxicity. Eur. J. Biochem. 269: 2591-2600. https://doi.org/10.1046/j.1432-1033.2002.02932.x
  17. Mengs, U., Gothel, D. and Leng-Peschlow, E. (2002) Mistletoe extracts standardized to mistletoe lectins in oncology: review on current status of preclinical research. Anticancer Res. 22: 1399-1407.
  18. Seo, J. H., Choi, Y. H., Kim, J. S., Kim, S. K., Choj, S. U., Kim, Y. S., Kim, Y. K., Kim, S. H. and Ryu, S. Y. (2004) Active principles of the methanol extract of Korean mistletoe responsible for the inhibitory effect on the proliferation of human tumor cell lines. Kor. J. Pharmacogn. 35: 134-138.
  19. Lee, J. Y., Kim, J. Y., Lee, Y. G., Byeon, S. E., Kim, B. H., Rhee, M. H., Lee, A., Kwon, M., Hong, S. and Cho, J. Y. (2007) In vitro immunoregulatory effects of Korean mistletoe lectin on functional activation of monocytic and macrophagelike cells. Biol. Pharm. Bull. 30: 2043-2051. https://doi.org/10.1248/bpb.30.2043
  20. Daniel, M. B. and Stuart, J. E. (1991) Protein methods. Wiley-Liss, Inc., N.Y.
  21. Choi, S. Y., Jung, S. K., Kim, S. K., You, Y. C., Lee, K. B., Kim, J. B., Kim, J. Y. and Song, K. S. (2004) An antioxidant homo-flavoyadorinin-B from Korean Mistletoe (Viscum album var. coloratum). J. Korean Soc. Appl. Bio. Chem. 47: 279-282.
  22. Microcal Inc. (1996) Microcal Software Origin Version 4.10 32bit Northampton, MA.
  23. Lyu, S. Y., Park, S. M., Choung, B. Y. and Park, W. B. (2000) Comparative Study of Korean (Viscum album var. coloratum) and European Mistltoes (Viscum album). Arch. Pharm. Res. 23: 592-598. https://doi.org/10.1007/BF02975247
  24. Hwang, K. H. (2007) Analysis of the characteristic materials of Korean Mistletoes (Viscum album var. coloratum). Report of research to Korea National Arboretum.