Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Antiobesity Effect of Mixture of Black Garlic and Garsinia cambogia Extracts in 3T3-L1 Adipocytes and L6 Skeletal Muscle Cells

  • Jung, Young-Mi (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University) ;
  • Lee, Dong-Sub (College of Health and Welfare, Kyungwoon University) ;
  • Lee, Seon-Ha (College of Health and Welfare, Kyungwoon University) ;
  • Jeoung, Nam-Ho (Department of Fundamental Medical & Pharmaceutical Sciences, CU-Leader's College, Catholic University of Daegu) ;
  • Kim, Bok-Jo (College of Health and Welfare, Kyungwoon University)
  • Received : 2012.09.06
  • Accepted : 2012.09.19
  • Published : 2012.09.30
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Abstract

The antiobesity effect of the mixture of black garlic and Garsinia cambogia extracts (BGG) was investigated by measuring the Oil red O staining and the expressions of adipogenic genes during preadipocyte differentiation by real-time PCR in the 3T3-L1 adipocytes. BGG reduced contents of Oil red O dye in the 3T3-L1 adipocytes. mRNA expression levels of SREBP1c, C/EBPa, aP2/FABP4, and $PPAR{\gamma}$ which are adipogenic transcription factor, in cells treated with BGG were also significantly down regulated. Also, the phosphorylation of AMP-activated protein kinase (AMPK) in L6 cells was more increased by BGG. These results indicate that BGG seems to be more attractive compound for application of industry than individual extracts such as black garlic and Garsinia cambogia, considering it has two effects not only inhibit the preadipocyte differentiation but also activate the phosphorylation of AMPK unlike other two compound.

Keywords

References

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