Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of Halogenated Compounds on in vitro Fermentation Characteristics in the Rumen and Methane Emissions

할로겐 화합물의 첨가가 반추위 발효성상과 메탄생성에 미치는 영향

  • Hwang, Hee-Soon (Division of Applied Life Science (BK21 program), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Ok, Ji-Un (Animal Nutrition Team, National Institute of Animal Science, RDA) ;
  • Lee, Shin-Ja (Department of Animal Science, Gyeongbuk Provincial College) ;
  • Chu, Gyo-Moon (Swine Science & Technology Center, Gyeongnam National University of Science and Technology) ;
  • Kim, Kyoung-Hoon (Animal Nutrition Team, National Institute of Animal Science, RDA) ;
  • Oh, Young-Kyoon (Animal Nutrition Team, National Institute of Animal Science, RDA) ;
  • Lee, Sang-Suk (Department of Animal Science & Biotechnology, Sunchon National University) ;
  • Lee, Sung-Sill (Division of Applied Life Science (BK21 program), Institute of Agriculture and Life Science, Gyeongsang National University)
  • 황희순 (경상대학교 응용생명과학부(BK21 program) & 농업생명과학연구원) ;
  • 옥지운 (농촌진흥청 국립축산과학원 영양생리팀) ;
  • 이신자 (경북도립대학 축산과) ;
  • 추교문 (경남과학기술대학교 양돈과학기술센터) ;
  • 김경훈 (농촌진흥청 국립축산과학원 영양생리팀) ;
  • 오영균 (농촌진흥청 국립축산과학원 영양생리팀) ;
  • 이상석 (순천대학교 동물자원과학과) ;
  • 이성실 (경상대학교 응용생명과학부(BK21 program) & 농업생명과학연구원)
  • Received : 2012.06.01
  • Accepted : 2012.08.07
  • Published : 2012.09.30
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Abstract

This study was conducted to evaluate effects of halogenated compounds on in vitro rumen fermentation characteristics and methane emissions. A fistulated Holstein cow of 650 kg body weight was used as a donor of rumen fluid. Five kinds of halogenated compounds (bromochloromethane (BCM), 2-bromoethane sulfonic acid (BES), 3-bromopropanesulfonic acid (BPS), chloroform (CLF), and pyromellitic diimide (PMDI) known to inhibit methyl-coenzyme M reductase activity were added to an in vitro fermentation incubated with rumen fluid. The microbial population including bacteria, protozoa, and fungi were enumerated, and gas production including methane and fermentation characteristics were observed in vitro. The pH values ranged from 6.25 to 6.72 in all the treatments, and these showed a similar level at 48 hr. The total gas production in the treatments showed a similar pattern with C at 48 hr, whereas methane production in the treatments was lower (p<0.05) than C. Concentrations of total volatile fatty acids (VFAs) and propionic acid were higher (p<0.05) in the treatments than in C at 12 hr. Therefore, halogenated compounds (BCM, BES, BPS, CLF, and PMDI) inhibited in vitro methane emissions by inhibiting methanogens in the rumen. Further studies on safety are needed.

본 연구는 할로겐 화합물의 첨가가 in vitro 상의 반추위 발효성상과 메탄생성에 미치는 영향에 대한 효과를 규명하고자 실시하였다. Italian rye grass 및 배합사료를 6:4의 비율로 급여한 반추위 cannula가 시술된 홀스타인에서 반추위액을 채취하여 사용하였고, 채취된 반추위액은 분쇄된 timothy (대조구; C)에 bromochloromethane (BCM구), 2-Bromoethanesulfonic acid (BES구), 3-Bromopropanesulfonic acid (BPS구), chloroform (CLF구) 및 Pyromellitic diimide (PMDI구)의 5가지 할로겐 화합물을 각각 1 ppm씩 첨가하여 in vitro 배양하였다. pH는 6.72에서 6.25 정도로 배양시간이 경과함에 따라 낮아지는 경향을 나타내었고, 배양 48시간에는 처리구간 차이가 없었다. 배양 48시간 후의 총 가스 발생량은 처리구간 유의적인(p<0.05) 차이는 없었고, BPS구를 제외한 모든 처리구에서의 메탄 발생량은 대조구에 비해 유의적으로(p<0.05) 감소하였다. 배양 12시간에서의 총 휘발성 지방산 및 propionic acid의 발생량은 처리구가 대조구에 비해 유의적으로(p<0.05) 높았다. 본 실험의 결과, 할로겐 화합물의 첨가는 반추위 내의 pH, 건물 소화율, 미생물 수 및 총 가스 발생량의 발효 성상에는 영향을 주지 않으면서 메탄의 발생량이 감소 되었다. 실험 결과를 종합해 보면, 할로겐 화합물 첨가는 반추위 내 pH, 가스 발생량, 반추위 미생물 성장량 및 propionic acid 모두 증가하였으며, 반추위내 메탄생성을 억제하였다. 앞으로 할로겐화합물과 다른 메탄억제 물질과 혼합하여 반추위 내 메탄생성 억제에 관한 구체적인 연구가 필요한 것으로 생각된다.

Keywords

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