Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Probiotic Potential of Pediococcus pentosaceus BCNU 9070

프로바이오틱 Pediococcus pentosaceus BCNU 9070 균주

  • Shin, Hwa-Jin (Department of Biology and Interdisciplinary Program in Biotechnology, Changwon National University) ;
  • Choi, Hye-Jung (Department of Biology and Interdisciplinary Program in Biotechnology, Changwon National University) ;
  • Kim, Dong-Wan (Department of Microbiology, Changwon National University) ;
  • Ahn, Cheol-Soo (Cho-A Pharm. Co, LTD.) ;
  • Lee, Young-Geun (Department of Food Science and Technology, Pusan National University) ;
  • Jeong, Young-Kee (Department of Biotechnology, Dong-A University) ;
  • Joo, Woo-Hong (Department of Biology and Interdisciplinary Program in Biotechnology, Changwon National University)
  • 신화진 (창원대학교 생물학과, 생명공학협동과정) ;
  • 최혜정 (창원대학교 생물학과, 생명공학협동과정) ;
  • 김동완 (창원대학교 미생물학과) ;
  • 안철수 (조아제약) ;
  • 이영근 (부산대학교 식품공학과) ;
  • 정영기 (부산대학교 식품공학과) ;
  • 주우홍 (창원대학교 생물학과, 생명공학협동과정)
  • Received : 2012.06.11
  • Accepted : 2012.08.16
  • Published : 2012.09.30
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Abstract

Lactic acid bacteria are generally recognized as beneficial probiotic organisms. Recent studies revealed that the potential of probiotic strains was essentially dependent on the bacterial-binding and adhesion capabilities to gut epithelial cells and the hydrophobicity of the cell surface. In this study, we screened some indigenous lactic acid bacteria from Kimchi and selected one lactic acid bacterium as a potential probiotic based on its cell surface hydrophobicity. Analysis of the 16S rRNA gene sequences of probiotic isolates indicated that the selected isolate (BCNU 9070 strain) was a member of Pediococcus pentosaceus. P. pentosaceus BCNU 9070 showed resistance to bile acids and acidic pH. The P. pentosaceus BCNU 9070 strain also inhibited the cell growth of six food-borne pathogens including Listeria monocytogenes and Shigella sonnei. In addition, the P. pentosaceus BCNU 9070 strain expressed bile salt hydrolase activity and showed an ability to assimilate cholesterol in vitro. On the basis of these results, P. pentosaceus BCNU 9070 is considered to have probiotic potential for applications in functional foodstuffs.

유산균은 일반적으로 프로바이오틱스 균주로 알려져 있다. 가능성이 있는 프로바이오틱스 균주는 세포결합과 부착능 즉, 장상피세포 부착능 및 세포표면의 소수성이 기초적으로 구비되어야 한다. 본 연구에서는 김치로부터 몇몇의 토착 유산균을 분리하였으며, 세포표면 소수성에 근거한 가능성이 있는 프로바이오틱스로서 유산균 한종을 선발하였다. 프로바이오틱스균주로서 선별한 분리균주(BCNU 9070)의 16S 리보좀DNA 염기서열을 분석한 결과 Pediococcus pentosaceus에 속하는 균주임이 확인되었다. P. pentosaceus BCNU 9070 균주는 위액과 담즙산에 대하여 내성을 가졌으며 또한 Listeria monocytogenes 및 Shigella sonnei를 포함하는 6종의 식중독 병원균에 대하여 생육저해활성도 나타내었다. 게다가 P. pentosaceus BCNU 9070 균주는 담즙산 가수분해활성 및 콜레스테롤 동화능도 있음이 확인되었다. 이들 결과를 기초로, P. pentosaceus BCNU 9070은 기능성 식품에 적용가능한 천연 프로바이오틱스 특성을 가지고 있다고 판단된다.

Keywords

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