Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Biochemical Analysis of Physiological Stress Induced by High Frequency Sound Treatment in the Beet Armyworm, Spodoptera exigua

고주파 처리에 따른 파밤나방(Spodoptera exigua)의 생리적 스트레스의 생화학적 분석

  • Kim, Yong-Gyun (Department of Bioresource Sciences, Andong National University) ;
  • Son, Ye-Rim (Department of Bioresource Sciences, Andong National University) ;
  • Seo, Sam-Yeol (Department of Bioresource Sciences, Andong National University) ;
  • Park, Bok-Ri (Department of Bioresource Sciences, Andong National University) ;
  • Park, Jung-A (Department of Bioresource Sciences, Andong National University)
  • 김용균 (안동대학교 자연과학대학 생명자원과학과) ;
  • 손예림 (안동대학교 자연과학대학 생명자원과학과) ;
  • 서삼열 (안동대학교 자연과학대학 생명자원과학과) ;
  • 박복리 (안동대학교 자연과학대학 생명자원과학과) ;
  • 박정아 (안동대학교 자연과학대학 생명자원과학과)
  • Received : 2012.01.28
  • Accepted : 2012.07.03
  • Published : 2012.09.01
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Abstract

High frequency sounds disrupt physiological processes, such as feeding behavior, development and immune responses of Spodoptera exigua. We analyzed high frequency sounds with respect to biochemical changes in S. exigua. High frequency sound (5,000 Hz, 95 dB) suppressed protein synthesis and secretion of midgut epithelium. It also significantly inhibited a digestive enzyme activity of phospholipase $A_2$. The gene expression of three different heat shock proteins and apolipophorin III was altered, particularly in midgut tissue in response to high frequency sound treatments. High frequency sound treatments significantly increased sugar and lipid levels in hemolymph plasma. These results suggest that high frequency sounds are a physiological stress that induces biochemical changes in S. exigua.

고주파 처리는 파밤나방(Spodoptera exigua)의 생리변화를 유발시켜 섭식행동, 발육 및 면역반응의 변화를 초래한다. 본 연구는 이러한 고주파의 영향을 파밤나방의 생화학적 변화를 통해 분석했다. 고주파(5,000 Hz, 95 dB) 처리는 중장 상피세포의 단백질 합성과 분비를 억제시켰다. 또한 이 고주파 처리는 중장의 인지질분해(phospholipase $A_2$) 소화효소의 활성을 현격하게 억제시켰다. 고주파 처리는 세 종류의 열충격단백질과 지질운송단백질(apolipophorin III)의 유전자 발현을 변동시켰고 이러한 변화는 중장 조직에서 뚜렷했다. 혈림프 혈장에 존재하는 지질 및 유리당의 함량이 고주파 처리에 의해 현격하게 증가했다. 이러한 결과는 고주파 처리가 파밤나방의 체내 생화학적 변화를 유발시켜 생리적 교란을 유도하는 스트레스로 작용한다는 것을 제시하고 있다.

Keywords

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