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Optimization of gamma-Aminobutyric Acid Bioconversion by Recombinant Escherichia coli

재조합 Escherichia coli를 이용한 gamma-Aminobutyric Acid 전환 반응 최적화

  • Le Vo, Tam Dinh (Department of Chemical Engineering, University of Ulsan) ;
  • Hong, Soon-Ho (Department of Chemical Engineering, University of Ulsan)
  • ;
  • 홍순호 (울산대학교 화학공학부)
  • Received : 2012.04.10
  • Accepted : 2012.04.25
  • Published : 2012.04.30

Abstract

In this study, the effects of pH, temperature, IPTG concentration and substrate (MSG) concentration on gamma-aminobutyric acid (GABA) production in engineered Escherichia coli were investigated. Glutamate decarboxylase and glutamate/GABA antiporter were overexpressed in GABA aminotransferase knock-out strain for GABA production. The result of optimization study showed the GABA bioconversion was optimized at pH 3.5, $30^{\circ}C$, 0.5 mM IPTG, 10 g/L MSG. At this condition, 5.23 g/L of final GABA concentration of was achieved from 10 g/L of MSG, which corresponded to a GABA yield of 85.77%.

Acknowledgement

Supported by : 울산대학교

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