Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Anti-apoptosis effects by Eimeria tenella infection in Madin-Darby bovine kidney cells

  • Lee, Hyun-A (Center for Animal Resource Development, Wonkwang University) ;
  • Hong, Sun-Hwa (Center for Animal Resource Development, Wonkwang University) ;
  • Chung, Yung-Ho (Department of Companion Animal and Animal Resources Science, Joongbu University) ;
  • Kim, Ok-Jin (Center for Animal Resource Development, Wonkwang University)
  • Received : 2012.05.02
  • Accepted : 2012.06.15
  • Published : 2012.06.30
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Abstract

Apoptosis is a host defense mechanism that the cell uses to limit production of infectious pathogens. Although many bacteria, viruses and parasites can induce apoptosis in infected cells, some pathogens usually exhibit the ability to suppress the induction of apoptosis in the infected cells. Sophisticated evasion strategies of obligate intracellular parasites, in particular prevention of host cell apoptosis, are necessary to ensure successful replication. To study the ability of Eimeria tenella in this regard, in vitro experiments were performed applying Madin-Darby bovine kidney (MDBK) cells as host cell. We have demonstrated that productive infection of adherent cell lines by E. tenella resulted in an anti-apototic effect. This phenomenon was confirmed using in situ terminal deoxynucleotidyl transferase-mediated (TdT) deoxyuridine triphosphates (dUTP)-fluorescein nick end labeling (TUNEL) assay to detect apoptosis. Therefore, E. tenella could complete its cycle of productive infection while inducing anti-apoptosis in the infected cells. This finding might have implications for the pathobiology of E. tenella and other Eimeria species.

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References

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