Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Analysis and Monitoring of Aflatoxin M1 in Milks

우유 중 아플라톡신 M1 오염도 조사연구

  • Park, Sung-Kug (Food Contaminants Division, Food Safety Evaluation Department, Korea Food and Drug Administration) ;
  • Kang, Young-Woon (Food Contaminants Division, Food Safety Evaluation Department, Korea Food and Drug Administration) ;
  • Kwon, Ki-Sung (Food Contaminants Division, Food Safety Evaluation Department, Korea Food and Drug Administration) ;
  • Lee, Gwang-Ho (Food Contaminants Division, Food Safety Evaluation Department, Korea Food and Drug Administration) ;
  • Kim, Mee-Hye (Food Contaminants Division, Food Safety Evaluation Department, Korea Food and Drug Administration)
  • 박성국 (식품의약품안전청 식품위해평가부 오염물질과) ;
  • 강영운 (식품의약품안전청 식품위해평가부 오염물질과) ;
  • 권기성 (식품의약품안전청 식품위해평가부 오염물질과) ;
  • 이광호 (식품의약품안전청 식품위해평가부 오염물질과) ;
  • 김미혜 (식품의약품안전청 식품위해평가부 오염물질과)
  • Received : 2011.11.25
  • Accepted : 2011.12.26
  • Published : 2012.04.30
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Abstract

Raw milk samples (n=28) obtained from milk tanks in 3 dairy plants of different regions and commercial milks (n=100) were collected from six cities. These samples were analyzed for the level of aflatoxin $M_1$ contamination using immunoaffinity columns and high performance liquid chromatography coupled with fluorescent detectors. Confirmation of aflatoxin $M_1$ ($AFM_1$) identified in positive samples was based on the formation of the hemiacetal derivative ($AFM_{2a}$) after derivatization with trifluroacetic acid. The average concentrations of aflatoxin $M_1$ in the raw milks were 25.1 ng/kg, and those values in commercial milks were 29.8 ng/kg. The highest level of aflatoxin $M_1$ in milk was 72.7 ng/kg. These results showed that the contamination of aflatoxin $M_1$ in milks consumed in the Korea was quite low compared to the standard in Korea Food Code (aflatoxin $M_1$ 500 ng/kg).

본 연구에서 적용한 시험법의 회수율은 97.4-122.5%, 상대표준편차(RSD)는 6%이내로서 적합한 시험법임을 확인하였다. 조사된 원유 및 시유의 검출농도는 아플라톡신 $M_1$이 평균 29.6 ng/kg(5.4-72.7 ng/kg)로 나타났으며, 원유 및 시유에 대한 아플라톡신 $M_1$ 오염수준은 현행 식품공전에서 정한 허용기준치인 500 ng/kg 보다 낮은 안전한 수준인 것으로 판단된다.

Keywords

References

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  2. Developments in mycotoxin analysis: an update for 2011-2012 vol.6, pp.1, 2013, https://doi.org/10.3920/WMJ2012.1492