Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Evaluation on Skin Irritation and Sensitization of Oxyresveratrol and Oxyresveratrol-3-O-glucoside Produced by Biotransformation of Morus alba Extract

상백피 추출물을 효소전환 하여 생성한 oxyresveratrol 및 oxyresveratrol-3-O-glucoside의 피부 자극성 및 감작성 평가

  • Park, Keun-Tae (Department of Biomedical Science, College of Health Science, Korea University) ;
  • Kim, Jeong-Keun (Department of Chemical Engineering and Biotechnology, Korea Polytechnic University) ;
  • Lim, Young-Hee (Department of Biomedical Science, College of Health Science, Korea University)
  • 박근태 (고려대학교 보건과학대학 임상병리학과) ;
  • 김정근 (한국산업기술대학교 생명화학공학과) ;
  • 임영희 (고려대학교 보건과학대학 임상병리학과)
  • Received : 2012.01.16
  • Accepted : 2012.02.14
  • Published : 2012.04.30
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Abstract

Stilbenes are known as antioxidants and some of them demonstrate anti-pigmentation activity. The purpose of the present study was to investigate whether two stilbene compounds produced by biotransformation of the extract of $Morus$ $alba$ root show skin irritation and sensitization. In skin irritation test, 1% oxyresveratrol (OXY), and 5% OXY, and 1% oxyresveratrol-3-$O$-glucoside (OXY-3) showed a P.I.I score of 0, 0.04, and 0, respectively. Accordingly, the two stilbenes were evaluated to be virtually 'non-irritant' materials. In a skin sensitization study by GPMT, 1% OXY, 5% OXY, and 1% OXY-3 did not cause edema and erythema at 24 h and 48 h after topical application and exhibited a sensitization score of 0 and a rate of 0%. Consequently, it was confirmed that OXY and OXY-3 had no contact allergic sensitization in GPMT. Therefore, OXY and OXY-3 might be potential candidates as skin-whitening agents without posing any serious side effects.

상백피 추출물의 연구결과로는 항산화효과, 미백, 주름개선 등의 효과가 있는 것으로 확인되었으나 화장품원료로 사용하기 위한 피부자극, 감작연구는 이루어지지 않은 상태이다. 본 연구에서는 guinea pig를 이용하여 피부 자극시험을 평가한 결과 OXY 및 OXY-3 처리군 모두에서 P.I.I 수치 0.5 이하에 해당하는 비자극성으로 판정되었다. 또한 GPMT법을 이용한 피부감작 시험 평가 결과 OXY 및 OXY-3 처리군 모두에서 발적, 홍반, 부종 등이 전혀 관찰되지 않았고, 반응의 빈도도 0%로 나타나 알러지 유발능이 없는 것으로 판명되었다. 결론적으로, 본 시험조건하에서 guinea pig에 대한 상백피 유래 미백성분들은 1% 이하의 농도에서 사용시 무자극성이며 면역반응에 의한 감작성 부작용을 유발하지 않는 것으로 판단되므로 1% 이하의 시료 물질을 화장품 원료 등에 적용시 약물에 의한 피부 자극성 및 면역반응에 의한 피부 감작성 부작용이 나타날 가능성은 비교적 낮을 것으로 판단된다.

Keywords

References

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