Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Experimental Studies on the inflammation-related diseases pharmacological effect of water and 70% ethanol extracts from Socheongnyong-tang

소청룡탕(小靑龍湯) 물 및 에탄올 추출물의 염증 관련 질환 약리 효능에 관한 실험적 연구

  • Jeon, Woo-Young (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Lee, Mee-Young (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Lim, Hye-Sun (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Shin, In-Sik (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Kim, Yeji (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Jin, Seong Eun (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Yoo, Sae-Rom (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Seo, Chang-Seob (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Kim, Jung-Hoon (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Ha, Hyekyung (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Jeong, Soo-Jin (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Kim, Ohn Soon (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Shin, NaRa (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Kim, Seong-Sil (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine)
  • 전우영 (한국한의학연구원 한약기초연구그룹) ;
  • 이미영 (한국한의학연구원 한약기초연구그룹) ;
  • 임혜선 (한국한의학연구원 한약기초연구그룹) ;
  • 신인식 (한국한의학연구원 한약기초연구그룹) ;
  • 김예지 (한국한의학연구원 한약기초연구그룹) ;
  • 진성은 (한국한의학연구원 한약기초연구그룹) ;
  • 유새롬 (한국한의학연구원 한약기초연구그룹) ;
  • 서창섭 (한국한의학연구원 한약기초연구그룹) ;
  • 김정훈 (한국한의학연구원 한약기초연구그룹) ;
  • 하혜경 (한국한의학연구원 한약기초연구그룹) ;
  • 정수진 (한국한의학연구원 한약기초연구그룹) ;
  • 김온순 (한국한의학연구원 한약기초연구그룹) ;
  • 신나라 (한국한의학연구원 한약기초연구그룹) ;
  • 김성실 (한국한의학연구원 한약기초연구그룹) ;
  • 신현규 (한국한의학연구원 한약기초연구그룹)
  • Received : 2012.09.28
  • Accepted : 2012.12.12
  • Published : 2012.12.30
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Abstract

Objectives : The present study aimed to investigate the pharmacological activity of water and ethanol (EtOH) extracts from Socheongnyong-tang (SCNT) on inflammation and its related disease. Methods : The cells were treated with nontoxic concentrations of water and EtOH extract from SCNT in BEAS-2B, HaCaT, RAW 264.7 and 3T3-L1 cells. These cells were stimulated by tumor necrosis facter (TNF)-${\alpha}$, TNF-${\alpha}$/interferon (IFN)-${\gamma}$, and lipopolysaccharide (LPS), respectively. 3T3-L1 cells were differentiated by insulin. After incubation, supernatant were collected and biological indicator measured by enzyme-linked immunosorbent assay. Results : Our results indicate that the water and EtOH extract of SCNT significantly inhibited the production of regulated on activation normal T-cell expression and secreted (RANTES) by treatment of TNF-${\alpha}$ in BEAS-2B cell, and significantly reduced the production of RANTES and macrophage-derived chemokine increased by treatment of TNF-${\alpha}$/IFN-${\gamma}$ in HaCaT cell. Moreover, those extracts significantly decreased the activity of nitric oxide and prostaglandin $E_2$ in LPS-induced RAW 264.7, and significantly inhibited the increased activity of glycerol-3-phosphate dehydrogenase and expression of leptin induced by differentiation in 3T3-L1 cell. Conclusions : These results indicate that both water and EtOH extract of SCNT has powerful effects on inflammation and its related disease. Therefore, SCNT can be developed as a potential pharmacological agent related various diseases. Although the significant effects were observed in both SCNT water and EtOH extract, the EtOH extract was more effective on most experiments than its water extract. Taken together, these findings indicate that the SCNT EtOH extract may have more potential pharmacological agent.

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References

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