Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Comparative Study on Chemical Composition and Biological Activities of Samchulkunbi-tang Decoction and Commercial Herbal Medicine

삼출건비탕 전탕액과 시판 한약제제의 비교 연구

  • Kim, Ohn Soon (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Seo, Chang-Seob (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Kim, Yeji (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine)
  • 김온순 (한국한의학연구원, 한약기초연구그룹) ;
  • 서창섭 (한국한의학연구원, 한약기초연구그룹) ;
  • 김예지 (한국한의학연구원, 한약기초연구그룹) ;
  • 신현규 (한국한의학연구원, 한약기초연구그룹)
  • Received : 2012.09.28
  • Accepted : 2012.12.12
  • Published : 2012.12.30
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Abstract

Objectives : The purpose of this study was to investigate the differences of chemical composition or biological activities between decoction and commercial herbal medicines of Samchulkunbi-tang (Shenzhujianpi-tang, SKT). Methods : The extracts of SKT from decoction (SKT1) and two different commercial extractive granules (SKT2 and SKT3) were prepared. The index components of SKTs were analyzed with HPLC. The antioxidant activities of SKTs were studied by measuring free radical scavenging activities on ABTS and DPPH. The anti-inflammatory effects were determined by measuring NO, $PGE_2$ and IL-6 in LPS-stimulated RAW 264.7 cells. Results : The contents of 7 components were 1.40-6.08 mg/g in SKT1, not detected-4.75 mg/g in SKT2, 0.03-1.46 mg/g in SKT3. The scavenging activities on ABTS and DPPH of herbal formulas were increased in dose-dependent manner (SKT1>SKT2>SKT3). SKT1 significantly inhibited $PGE_2$ and IL-6 production and SKT3 slightly inhibited $PGE_2$ production in LPS-stimulated RAW264.7 cells. SKT2 showed no inhibitory effects on production of inflammatory mediators such as $PGE_2$ and IL-6. Conclusions : These results demonstrate that the decoction of SKT has more strong anti-oxidant and anti-inflammatory effects than that of commercial herbal medicines consistent with the contents of index components.

Keywords

References

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