Journal of Plant Biotechnology

  • 제38권3호
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  • Pages.198-202
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  • 2011
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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Agrobacterium에 의한 오이 형질전환에서 자엽절 절편의 이용

The use of cotyledonary-node explants in Agrobacterium tumefaciensmediated transformation of cucumber (Cucumis sativus L.)

  • 장현아 (남부대학교 약용식물형질전환연구소) ;
  • 김현아 (한국생명공학연구원) ;
  • 권석윤 (한국생명공학연구원) ;
  • 최동욱 (전남대학교 생물교육과) ;
  • 최필선 (남부대학교 약용식물형질전환연구소)
  • Jang, Hyun-A (Department of Oriental Pharmaceutical Development, Nambu University) ;
  • Kim, Hyun-A (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kwon, Suk-Yoon (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Choi, Dong-Woog (Department of Biological Education, Jeonnam National University) ;
  • Choi, Pil-Son (Department of Oriental Pharmaceutical Development, Nambu University)
  • 투고 : 2011.06.29
  • 심사 : 2011.07.30
  • 발행 : 2011.09.30
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초록

Agrobacterium과 자엽절 절편 공동배양으로 오이 형질전환체를 생산하였다. 오이 자엽절 절편 (c.v. Eunsung)은 선발 마커로서 nptII유전자와 유용유전자로서 DQ유전자가 포함된 pPZP211를 EHA101에 형질전환하여 공동 배양하였다. 3회 반복실험으로부터 평균 형질전환 빈도는 4.01%를, 최대 빈도는 5.97%를 보여 주었다. Paromomycin항생제 저항성을 갖는 9개의 식물체를 선발과정을 통해 얻었으며, Southern blot 분석에 의해 6개 식물체의 genome에 nptII유전자자 안정적으로 도입되어 있음을 확인할 수 있었다.

Agrobacterium tumefaciens-mediated cotyledonary-node explants transformation was used to produce transgenic cucumber. Cotyledonary-node explants of cucumber (Cucumis sativus L. cv., Eunsung) were co-cultivated with Agrobacterium strains (EHA101) containing the binary vector (pPZP211) carrying with CaMV 35S promoter-nptII gene as selectable marker gene and 35S promoter-DQ gene (unpublished data) as target gene. The average of transformation efficiency (4.01%) was obtained from three times experiments and the maximum efficiency was shown at 5.97%. A total of 9 putative transgenic plants resistant to paromomycin were produced from the cultures of cotyledonary-node explants on selection medium. Among them, 6 transgenic plants showed that the nptII gene integrated into each genome of cucumber by Southern blot analysis.

키워드

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