• Title/Summary/Keyword: cotyledonary-node

Search Result 34, Processing Time 0.03 seconds

Early Ontogeny of Vascular Cambium in Cotyledonary Node of Seedlings in Pinus koraiensis S. et Z. (잣나무(Pinus koraiensis S. et Z.) 유식물의 자엽절에서 유관속 형성층의 초기 발생)

  • 홍성식
    • Journal of Plant Biology
    • /
    • v.36 no.1
    • /
    • pp.29-34
    • /
    • 1993
  • Observation was made on early ontogeny of vascular cambium in the cotyledonary node of Pinus koraiensis seedling in order to clarify the cambial ontogeny of the node which was hardly elongated. In transverse view, the derivatives by the early periclinal divisions at the outer region of the procambial strands differentiated to protophloem with establishing a certain degree of radial seriations. Later, some cells in the central region of the procambial strands begin divide periclinally, eventually the cells, differentiate gradually to the vascular cambium. In tangential view, early procambium is consisted of homogeneous short cells with transverse end walls which are tansformed into the long cells and short cells gradually. The long cells continue intrusive growth and the short cells repeat transverse division. Finally, the long and short cells differentiated to cambial fusiform initials and ray initials respectively. In tangential section, the differentiation pattern of cambial initials resembles that of the stem of Ginkgo biloba. But in transverse view, the characteristics in the origin of vascular cambium of the cotyledonary node resembles that of root. The vascular cambium in cotyledonary node is completed after eleven prophylls were formed.

  • PDF

Early Ontogeny of Vasuclar Cambium in Cotyledonary Node of Ginkgo biloba L. Seedlings (은행나무(Ginkgo biloba L.) 유식물의 자엽절에서 유관속형성층의 초기발생)

  • 소웅영
    • Journal of Plant Biology
    • /
    • v.35 no.4
    • /
    • pp.359-364
    • /
    • 1992
  • The vascular cambium in Ginkgo biloba seedling began to differentiate in the cotyledonary node, and then the differentiation proceeded bidirectionally from the cotyledonary node toward the stem and root. In tangential view, procambium at the early developmental stage was a homogeneous structure consisted of almost similar cells in shape, and at the later stage the procambium became a heterogeneous one consisted of long cells and short cells. Such a differentiation pattern in the cotyledonary node was similar to that in the stem. However, it was different from that in the root. Fusiform initials and ray initials consisting the vascular cambium were originated from the long cells and the short cells, respectively. The long cells and the fusiform initials in the cotyledonary node were shorter and wider than those in the first internode.ernode.

  • PDF

Influence of Antibiotics on Shoot Regeneration and Agrobacteium Suppression Using Cotyledonary Node in Korean Soybean Cultivars

  • Kim, Kyung-Hee;Lee, Jae-Eun;Kwon, Young-Up;Lee, Byung-Moo
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.54 no.3
    • /
    • pp.307-313
    • /
    • 2009
  • Mature dry seeds of Korean cultivars, Daepungkong, Muhankong, Myeongjunamulkong, Somyeongkong, Sowonkong, Jinpumkong, and Pungsannamulkong were used. The influence of antibiotics on elimination of Agrobacterium growth and shoot regeneration was estimated with cotyledonary node. Cefotaxime and timentin at the concentration of 250 and 500 mg/l suppressed Agrobacterium, especially cefotaxime was an efficient antibiotic to suppress Agrobacterium in all cultivars. While carbenicillin and timentin at the concentration of 50 and 100 mg/l were not sufficient to control the development of Agrobacterium, respectively. Cefotaxime and timentin represented high shoot formation rates compared with carbenicillin. Carbenicillin at low concentrations did not effectively suppress Agrobacterium and also had no effect on shoot development. Cefotaxime at the concentration of 250 mg/l showed maximum frequency of shoot regeneration in cvs. Somyeongkong and Sowonkong. Furthermore, on medium containing cefotaxime, shoot was more quickly formed than the other antibiotics. The use of cefotaxime was very useful for elimination of Agrobacterium growth with cotyledonary node of Korean soybean cultivars.

The use of cotyledonary-node explants in Agrobacterium tumefaciensmediated transformation of cucumber (Cucumis sativus L.) (Agrobacterium에 의한 오이 형질전환에서 자엽절 절편의 이용)

  • Jang, Hyun-A;Kim, Hyun-A;Kwon, Suk-Yoon;Choi, Dong-Woog;Choi, Pil-Son
    • Journal of Plant Biotechnology
    • /
    • v.38 no.3
    • /
    • pp.198-202
    • /
    • 2011
  • Agrobacterium tumefaciens-mediated cotyledonary-node explants transformation was used to produce transgenic cucumber. Cotyledonary-node explants of cucumber (Cucumis sativus L. cv., Eunsung) were co-cultivated with Agrobacterium strains (EHA101) containing the binary vector (pPZP211) carrying with CaMV 35S promoter-nptII gene as selectable marker gene and 35S promoter-DQ gene (unpublished data) as target gene. The average of transformation efficiency (4.01%) was obtained from three times experiments and the maximum efficiency was shown at 5.97%. A total of 9 putative transgenic plants resistant to paromomycin were produced from the cultures of cotyledonary-node explants on selection medium. Among them, 6 transgenic plants showed that the nptII gene integrated into each genome of cucumber by Southern blot analysis.

Optimized Shoot Induction and Histological Study of in vitro Cultured Korean Soybean Cultivars

  • Kantayos, Vipada;Bae, Chang-Hyu
    • Korean Journal of Plant Resources
    • /
    • v.32 no.3
    • /
    • pp.237-243
    • /
    • 2019
  • Soybean is the one of recalcitrant legume species for shoot induction. Shoot regeneration via direct organogenesis was investigated in five soybean cultivars, 'Dawon', 'Pungsan', 'Daewon', 'Taekwang' and 'Chongdoo 1' by using cotyledonary node explants. Out of 5 soybean cultivars, an efficient shoot regeneration condition was developed in the two soybean cultivars, 'Dawon' and 'Pungsan'. When various kinds of plant growth regulators with different concentration were estimated, the optimum medium condition for shoot induction in both soybean cultivars was MS + B5 vitamin supplemented with BA at concentration 2 mg/L. In addition, shoot formation efficiency was increased with 97.09% and 93.88% by the pretreatment of BA onto the explants before in vitro culture in both cultivars. Shoot induction in 'Dawon' cultivar was originated from epidermal tissue and sub-epidermal layers when histological changes were investigated under shoot regeneration after culturing cotyledonary node segments on shoot induction medium for 0 to 21 days. Especially, cell dedifferentiation was observed from parenchyma cells to meristematic cell in 3-day cultured segments.

Optimization of Shoot Induction, Histological Study and Genetic Stability of in vitro Cultured Pisum sativum cv. 'Sparkle'

  • Kantayos, Vipada;Bae, Chang-Hyu
    • Korean Journal of Plant Resources
    • /
    • v.32 no.1
    • /
    • pp.19-28
    • /
    • 2019
  • An efficient shoot regeneration condition for pea cv. 'Sparkle' was developed by using optimum explant, plant growth regulator concentrations, and pretreatment of BA onto explant. The average shoot number per explant showed the highest on two kinds of shoot induction media (MSB5 media containing 2 mg/L BA and a combination of 2 mg/L BA and 1 mg/L TDZ) when cotyledonary node explants were cultured. Moreover, the pretreatment of explant in 200 mg/L BA solution was found to be more effective in shoot induction than that of non-pretreatment. By histological study, cell division and proto-meristem were formed near the surface of the sub-epidermal and epidermal cell layers of cotyledonary node in earlier than 3 days after culture. The analysis of genetic stability of regenerants by using thirteen ISSR markers showed that in vitro regenerated plants showed polymorphism with 8.3% compared with their mother plants.

Plant Regeneration from in vitro Tissue Culture of Soybean Seedling (콩 유묘의 조직배양에 의한 식물체 분화)

  • Kim, Yong-Ho;Kim, Seok-Dong;Hong, Eun-Hi
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.37 no.5
    • /
    • pp.419-424
    • /
    • 1992
  • To study the capacity of callus and shoot formation on seedling stage in soybean, excised hypocotyl, epicotyl, shoot tip, cotyledonary node and primary leaf were cultured on artificial media (MS and B$_{5}$ medium) supplemented with several hormones. Regeneration of shoots was fairly successful from shoot tip and cotyledonary node tissues in soybean. These shoots could be rooted in vitro through tissue culture technique and transplanted normally into soil. Hypocotyl and epicotyl tissues formed only callus, of which growth and appearance were different according to the kinds of media and additives. A small number of shoots were formed from primary leaf tissues, but they did not develop further.r.

  • PDF

Production of Transgenic Melon from the Cultures of Cotyledonary-Node Explant Using Agrobacterium-Mediated Transformation (Agrobacterium 공동 배양을 통한 자엽절 절편 배양으로부터 멜론 형질전환체 생산)

  • Cho Mi-Ae;Song Yun-Mi;Park Yun-Ok;Ko Suck-Min;Min Sung-Ran;Liu Jang-Ryol;Lee Jun-Haeng;Choi Pil-Son
    • Journal of Plant Biotechnology
    • /
    • v.32 no.4
    • /
    • pp.257-262
    • /
    • 2005
  • Agrobacterium tumefaciens-mediated cotyledonary-node explants transformation was used to produce transgenic melon. Cotyledonary-node explants of melon (Cucumis melo L. cv. Super VIP) were co-cultivated with Agrobacterium strains (LBA4404, GV3101, EHA101) containing the binary vector (pPTN289) carrying with CaMV 35S promoter-gus gene as reporter gene and NOS promoter-bar gene conferring resistance to glufosinate (herbicide Basta) as selective agent, and the binary vector (pPTN290) carrying with Ubiquitin promoter-GUS gene and NOS promoter-nptll gene conferring resistance to paromomycin as selective agent, respectively. The maximum transformation efficiency (0.12%) was only obtained from the cotyledonary-node explants co-cultivated with EHA101 strain (pPTN289) on selection medium with 5 mg/L glufosinate and not produced a transgenic melon from the cotyledon or cotyledonary-node co-cultivated with other strains. Finally, five plants transformed showed the resistance in glufosinate antibiotic and the GUS positive response in leaf ($T_0$), flower ($T_0$), seeds ($T_1$) and plantlet ($T_1$). Southern blot analysis revealed that the gus gene integrated into each genome of transgenic melon.

Differentiation of the Vascular System in Mature Embryo and Seedling of Prunus davidiana FR. (산복사나무(Prunus davidiana FR.)의 성숙배와 유식물에 있어서 유관속계의 분화)

  • 홍성식
    • Journal of Plant Biology
    • /
    • v.28 no.4
    • /
    • pp.285-296
    • /
    • 1985
  • To examine the differentiation and transition of vascular system in Prunus davidiana FR., the mature embyro and developing seedlings were embedded in paraplast and treated by clearing method. In mature embryo, the procambium was connected with the epicotyl-hypocotyl-radicle axis and cotyledons, whereas protophloem and protoxylem were restricted primarily to the mid-vein and two lateral veins of the cotyledonary base. With the onset of germination, protophloem and protoxylem were differentiated both acropetally and basipetally from the cotyledonary base. The first and second leaf traces appeared in the cotyledonary node, and then differentiated bidirectionally toward the epicotyl and the root tip. The 3rd to 6th leaf traces were connected with the cotyledonary traces in hypocotyl. At the part of the root tip, the xylem was a diarch. As the first and second leaf traces were superimposed at the middle part of the root, the diarch xylem was changed to a tetrarch. As the cotyledonary traces were diverged below the root base, the tetrarch xylem was changed to an octarch. It was suggested that the vascular system of the epicotyl might be superimposed on that of the cotyledon-hypocotyl-root during the formation of the primary vascular system of Prunus davidiana.

  • PDF

Inflorescence Habits of Different Plant Types in Peanut (땅콩의 초형별 개화습성)

  • 배석복;김종진
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.42 no.6
    • /
    • pp.841-848
    • /
    • 1997
  • This study was conducted to investigate the inflorescence habit mainly on major in farmer's peanut cultivar of the Shinpung plant type "Daekwang" of sequential branching and the Virginia plant type "Daepung" of alternate branching until maximum flowering dates. All flowers of two pairs of opposite branch in "Daekwang" showed almost symmetrical flowering order and in "Daepung" also showed similar tendency though it had somewhat irregalar alternate branching type in opposite branches. The lowest node in each branch had seven flowers while upper nodes had less flowers in this period. The first flower in "Daekwang" was born at first node of second branch of cotyledonary branches compared to those of "Daepung" at first reproductive branch of cotyledonary branches. One pair of cotyledonary branches had two-thirds of total flowers in two cultivars. Dates of average flower proceeding between nodes in each branch of "Daekwang" were appeared 4.32 days in main axil, 1st and 2nd opposite primary branches were 4.44 and 5.13 days, and 1st and 2nd secondary branches were 5.81 and 6.41 days respectively, but "Daepung" showed similar flower proceeding dates in all branches. but "Daepung" showed similar flower proceeding dates in all branches.

  • PDF