Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Biodegradation of Organochlorine Insecticide Endosulfan by the Fungus Eutypella sp. KEF-1

  • Lee, Jung-Bok (Department of Optometry, Kundong University) ;
  • Park, Sang-Yeul (Department of Optometry, Kundong University) ;
  • Shin, Kee-Sun (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Jeon, Chun-Pyo (Dept. of Medicine Quality analysis, Andong Science College) ;
  • Kim, Jang-Eok (School of Applied Bioscience, Kyungpook National University) ;
  • Kwon, Gi-Seok (School of Bioresource Sciences, Andong National University)
  • Received : 2011.06.02
  • Accepted : 2011.06.17
  • Published : 2011.06.30
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Abstract

BACKGROUD: ${\alpha}$- and ${\beta}$- Endosulfan isomers of endosulfan, an endocrine disrupting chemical, are widely used cyclodiene organochlorine pesticide in worldwide, and it has widespread application in agriculture and can contaminate river-system as runoff from soil or aerial deposition METHOD AND RESULTS: In this study, an attempt was made to isolate an endosulfan degrading fungus from endosulfan-polluted agricultural soil. Through repetitive enrichment and successive subculture in media containing endosulfan and its metabolites as the sole carbon source, a fungus designated KEF-1 was isolated. Based on phylogenetic analysis, strain KEF-1 was assigned to the genus Eutypella. Also, the ITS (internal transcribed spacer) sequences of KEF-1 were submitted to GenBank under accession number EF581006. In potato dextrose broth containing 8 ${\mu}g$/mL endosulfan, strain KEF-1 completely degraded the endosulfanin 12 days. CONCLUSION: These results suggested that Eutypella sp. KEF-1 has potential as a biocatalyst for endosulfan bioremediation

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References

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