Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Contents of Poncirin and Naringin in Fruit of Poncirus Trifoliata according to Different Harvesting Times and Locations for Two Years

2년 동안 지실의 채취시기 및 지역에 따른 Poncirin과 Naringin의 함량

  • Lee, Sul-Lim (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Seo, Chang-Seob (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Kim, Jung-Hoon (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
  • 이설림 (한국한의학연구원 한약EBM연구센터) ;
  • 서창섭 (한국한의학연구원 한약EBM연구센터) ;
  • 김정훈 (한국한의학연구원 한약EBM연구센터) ;
  • 신현규 (한국한의학연구원 한약EBM연구센터)
  • Received : 2011.04.16
  • Accepted : 2011.06.16
  • Published : 2011.06.30
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Abstract

A high performance liquid chromatography (HPLC) method was developed for simultaneous determination of two major flavonoid glycosides (poncirin and nanringin) in Poncirus trifoliata Raf. by different harvesting times and locations for two years. A SunFire $C_{18}$ column (4.6 mm${\times}$250 mm, 5 ${\mu}M$) was used at $40^{\circ}C$ for the determination of poncirin and naringin. The mobile phase using gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid. Flow rate was 1.0 mL/min and injection volume was 10 ${\mu}l$. The chromatogram was monitored by photodiode array (PDA) detection at 280 nm for the identification of two flavonoid glycosides in P. trifoliata. The contents of the two components in P. trifoliata ranged from 0.32~13.02%.

Keywords

References

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