KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Enhancement of Saccharification Yield of Ulva pertusa Kjellman by High Pressure Homogenization Process for Bioethanol Production

구멍갈파래의 고압 균질 전처리 공정을 통한 바이오에탄올 생산용 당화수율 증진

  • Choi, Woon-Yong (Department of Biomaterial Engineering, Kangwon Natl. Univ.) ;
  • Lee, Choon-Geun (Department of Biomaterial Engineering, Kangwon Natl. Univ.) ;
  • Ahn, Ju-Hee (Department of Biomaterial Engineering, Kangwon Natl. Univ.) ;
  • Seo, Yong-Chang (Medical & Bio-Material Research Center and Department of Biomaterials Engineering College of Bioscience and Biotechnology, Kangwon National University) ;
  • Lee, Sang-Eun (Department of Biotechnology, Chungju National University) ;
  • Jung, Kyung-Hwan (Department of Biotechnology, Chungju National University) ;
  • Kang, Do-Hyung (Korea Ocean Research & Development Institute) ;
  • Cho, Jeong-Sub (DooSan EcoBizNet) ;
  • Choi, Geun-Pyo (Department of Food Processing and Bakery, Gangwon Provincial College) ;
  • Lee, Hyeon-Yong (Department of Biomaterial Engineering, Kangwon Natl. Univ.)
  • 최운용 (강원대학교 생물소재공학전공) ;
  • 이춘근 (강원대학교 생물소재공학전공) ;
  • 안주희 (강원대학교 생물소재공학전공) ;
  • 서용창 (강원대학교 의료, 융복합 인재양성센터) ;
  • 이상은 (충주대학교 바이오산업학과) ;
  • 정경환 (충주대학교 바이오산업학과) ;
  • 강도형 (한국해양연구원) ;
  • 조정섭 (두산에코비즈넷) ;
  • 최근표 (강원도립대학교 식품가공제과제빵과) ;
  • 이현용 (강원대학교 생물소재공학전공)
  • Received : 2011.08.03
  • Accepted : 2011.09.20
  • Published : 2011.10.31
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Abstract

This study was investigated to improve the saccharification yield of Ulva pertusa Kjellman by the high pressure homogenization process. It was found that the high pressure homogenization pretreatment effectively destructed the cell wall structures only by using water. The high pressure homogenization process was operated under various conditions such as 10000, 20000 or 30000 psi with different recycling numbers. The optimal condition was determined as 30000 psi and 2 pass of recycling numbers and the sugar conversion yields were 16.02 (%, w/w) of glucose and 14.70 (%,w/w) of xylose, respectively. In the case of enzymatic treating the hydrolyzates with 5 FPU/glucan of celullase and 100 units/mL of amyloglucosidase, 65.8% of carbohydrates was converted into glucose. Using the hydrolysates of Ulva pertusa Kjellman, 48.7% of ethanol was obtained in the culture S.cerevisiae. These results showed that the high pressure homogenization process could efficiently hydrolyze the marine resource by using only water for bioethanol production.

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References

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