Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Biochemical Characterization of the Exopolysaccharide Purified from Laetiporus sulphureus Mycelia

  • Seo, Min-Jeong (Department of Medical Bioscience, Dong-A University) ;
  • Kang, Byoung-Won (Medi-Farm Industrialization Research Center, Dong-A University) ;
  • Park, Jeong-Uck (Medi-Farm Industrialization Research Center, Dong-A University) ;
  • Kim, Min-Jeong (Medi-Farm Industrialization Research Center, Dong-A University) ;
  • Lee, Hye-Hyeon (Medi-Farm Industrialization Research Center, Dong-A University) ;
  • Choi, Yung-Hyun (Department of Biochemistry, College of Oriental Medicine, Dong-Eui University) ;
  • Jeong, Yong-Kee (Department of Medical Bioscience, Dong-A University)
  • Received : 2011.06.24
  • Accepted : 2011.08.12
  • Published : 2011.12.28
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Abstract

The extracellular polysaccharide (EPS) was isolated from mycelial cultures of Laetiporus sulphureus var. miniatus and purified by DEAE cellulose and Sephadex G-50 column chromatography. The purified EPS (EPS-2-1) was composed of only glucose units and its molecular mass was 6.95 kDa. The chemical structure of EPS-2-1 consisted of a main chain containing ($1{\rightarrow}4$)-Glcp units with branches at the C-6 position of the chain carrying-Glcp-($1{\rightarrow}4$)-linked residues. The effect of purified EPS on immunomodulatory genes and proteins of the Bcl-2 family was observed using cultured U937 human leukemia cells. Of note, the levels of Bax and Bad proteins treated with the EPS (4 mg/ml) were approximately 23- and 18-times higher than those in non-treated cells, respectively. These results may suggest that the EPS purified from the mushroom L. sulphureus is associated with the activation of immunomodulatory mediators, Bax and Bad proteins.

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