Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Histopathological observation of the crucian carp Carassius auratus with infected leech Limnotrachelobdella sinensis by scanning electron microscope

주사전자현미경을 이용한 거머리, Limnotrachelobdella sinensis 감염 붕어, Carassius auratus의 조직병리학적 관찰

  • Park, Jung-Jun (Pathology Division, National Fisheries Research and Development Institute) ;
  • Byoun, Ju-Young (Safety and Environment Department, Korea Hydro and Nuclear Power Co., Ltd.) ;
  • Kim, Jung-Ho (Faculty of Marine Bioscience and Technology, Gangneung-Wonju Nat. Univ.) ;
  • Choi, Hye-Sung (Pathology Division, National Fisheries Research and Development Institute) ;
  • Park, Myoung-Ae (Pathology Division, National Fisheries Research and Development Institute) ;
  • Kim, Seok-Ryel (Pathology Division, National Fisheries Research and Development Institute)
  • 박정준 (국립수산과학원 병리연구과) ;
  • 변주영 ((주)한국수력원자력) ;
  • 김정호 (강릉원주대학교 해양과학기술학부) ;
  • 최혜승 (국립수산과학원 병리연구과) ;
  • 박명애 (국립수산과학원 병리연구과) ;
  • 김석렬 (국립수산과학원 병리연구과)
  • Received : 2011.08.18
  • Accepted : 2011.09.19
  • Published : 2011.12.31
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Abstract

All of the crucian carp, Carassius auratus and 50% of common carp. Cyprinus carpio examined in this study were infected with the leeches, Limnotrachelobdella sinensis. Especially, the infection of C. carpio with L. sinensis was the first report in Korea. The gill of C. auratus showed increased hydrophic degeneration of epithelial cell in the filament, blood congestion, hyperplasia of epithelial cell in the filament and lamellae. In the SEM observation, gill filament was transformed to the cylinder form by the lamellae fusion. The lamellae surface showed degeneration, fragmentation of microridges. The extracellular cartilaginous matrix of the filaments was exposed by the collapse of epidermal layer. In the 18S rRNA analysis of L. sinensis, the relationships among these groups are not clear and not concord with their morphological classification.

충북 청원군 병천천에서 채집된 시료 중 붕어 5마리에서 모두 거머리가 관찰되었고, 잉어는 10마리 중 5마리에서 거머리의 기생이 확인되었다. 잉어에서의 거머리 기생은 국내에서 처음으로 확인되었다. 붕어 아가미는 거머리의 흡혈로 인하여 새엽 상피세포의 증가, 새판에 존재하는 모세혈관의 확장, 새판 일부분과 새엽이 소실, 새판 상피세포들은 수종변성(hydrophic degeneration), 울혈, 새판 및 새엽 상피세포의 증식 등이 관찰되었다. SEM으로 관찰한 결과 새판이 인접하는 새판과 점진적으로 융합되어, 결국은 새엽 하나가 원통모양으로 변형되었다. 또한, 붕어 아가미 새엽의 자유면에 존재하는 미세융기의 부분적인 파괴가 관찰되었다. 새엽 상피층이 파괴된 부분에는 연골기질이 외부로 노출되었고, 그 주변에는 다수의 혈구들이 산재하고 있었다. L. sinensis의 18S rRNA 분석은 형태학적 분류와 일치하지 않았다.

Keywords

References

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