한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제15권4호
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  • Pages.339-347
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  • 2011
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
권호 표지

Long-term Cryopreservation of Mesenchymal Stem Cells Derived from Human Eyelid Adipose and Amniotic Membrane: Maintenance of Stem Cell Characteristics

  • Song, Yeon-Hwa (Dept. of Biotechnology, Seoul Women's University) ;
  • Park, Se-Ah (Dept. of Biotechnology, Seoul Women's University) ;
  • Yun, Su-Jin (Dept. of Biotechnology, Seoul Women's University) ;
  • Yang, Hye-Jin (Dept. of Biotechnology, Seoul Women's University) ;
  • Yoon, A-Young (Dept. of Biotechnology, Seoul Women's University) ;
  • Kim, Haek-Won (Dept. of Biotechnology, Seoul Women's University)
  • 투고 : 2011.11.21
  • 심사 : 2011.12.09
  • 발행 : 2011.12.31
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초록

Human eyelid adipose-derived stem cells (hEAs) and amniotic mesenchymal stem cells (hAMs) are very valuable sources for the cell therapeutics. Both types of cells have a great proliferating ability in vitro and a multipotency to differentiate into adipocytes, osteoblasts and chondrocytes. In the present study, we evaluated their stem cell characteristics after long-time cryopreservation for 6, 12 and 24 months. When frozen-thawed cells were cultivated in vitro, their cumulative cell number and doubling time were similar to freshly prepared cells. Also they expressed stem cell-related genes of SCF, NANOG, OCT4, and TERT, ectoderm-related genes of NCAM and FGF5, mesoderm/endoderm-related genes of CK18 and VIM, and immune-related genes of HLA-ABC and ${\beta}$2M. Following differentiation culture in appropriate culture media for 2-3 weeks, both types of cells exhibited well differentiation into adipocyte, osteoblast, and chondrocyte, as revealed by adipogenic, osteogenic or chondrogenic-specific staining and related genes, respectively. In conclusion, even after long-term storage hEAs and hAMs could maintain their stem cell characteristics, suggesting that they might be suitable for clinical application based on stem cell therapy.

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참고문헌

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