한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제15권4호
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  • Pages.331-338
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  • 2011
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
권호 표지

miRNA-222 Modulates Differentiation of Mouse Embryonic Stem Cells

  • Ahn, Hee-Jin (Department of Biomedical Science, College of Life Science, CHA University) ;
  • Jung, Jee-Eun (Department of Biomedical Science, College of Life Science, CHA University) ;
  • Park, Kyung-Soon (Department of Biomedical Science, College of Life Science, CHA University)
  • 투고 : 2011.11.15
  • 심사 : 2011.12.02
  • 발행 : 2011.12.31
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초록

MicroRNAs (miRNAs) function as a key regulator of diverse cellular functions. To find out novel miRNAs that promote the differentiation of mouse embryonic stem cells (mESCs), we compared the miRNAs expression profiles of mESCs under self-renewal vs. differentiation states. We noticed that miR-222 was highly expressed during the differentiation of mESCs. Quantitative RT-PCR analysis revealed that expression of miR-222 was up-regulated during the embryonic bodies formation and retinoic acid -dependent differentiation. When miR-222 was suppressed by antogomiR-222, the differentiation of mESCs was delayed compared to control. Self-renewal marker expression or cell proliferation was not affected but the expression of lineage specific marker was suppressed by the treatment of miR-222 inhibitor during the differentiation of mESCs. Taken together, these results suggest that miR-222 functions to promote the differentiation of mESCs by regulating expression of differentiation related genes.

키워드

참고문헌

  1. Acunzo M, Visone R, Romano G, Veronese A, Lovat F, Palmieri D, Bottoni A, Garofalo M, Gasparini P, Condorelli G, Chiariello M, Croce CM (2011) MiR-130a targets MET and induces TRAIL-sensitivity in NSCLC by downregulation miR-221 and 222. Oncogene Epub.
  2. Barroso-delJesus A, Romero-López C, Lucena-Aguilar G, Melen GJ, Sanchez L, Ligero G, Berzal-Herranz A, Menendez P (2008) Embryonic stem cell-specific miR302-367 cluster: human gene structure and functional characterization of its core promoter. Mol Cell Biol 28:6609-6619. https://doi.org/10.1128/MCB.00398-08
  3. Card DA, Hebbar PB, Li L, Trotter KW, Komatsu Y, Mishina Y, Archer TK (2008) Oct4/Sox2-regulated miR-302 targets cyclin D1 in human embryonic stem cells. Mol Cell Biol 28:6426-6438. https://doi.org/10.1128/MCB.00359-08
  4. Cardinali B, Castellani L, Fasanaro P, Basso A, Alema S, Martelli F, Falcone G (2009) Microrna-221 and microrna-222 modulate differentiation and maturation of skeletal muscle cells. PLoS One 4:e7607. https://doi.org/10.1371/journal.pone.0007607
  5. Evans MJ, Kaufman MH (1981) Establishment in culture of pluripotential cells from mouse embryos. Nature 292:154-156. https://doi.org/10.1038/292154a0
  6. Fraichard A, Chassande O, Bilbaut G, Dehay C, Savatier P, Samarut J (1995) In vitro differentiation of embryonic stem cells into glial cells and functional neurons. J Cell Science 108:3181-3188.
  7. Gustavo T, Juan CIB (2010) MicroRNAs in embryonic stem cell function and fate. Gene & Development 24:2732-2741. https://doi.org/10.1101/gad.1982910
  8. Junlin Q, Jenn-Yah Y, Halyna RS, Julie M, Amy JW, Sudeshna S, Wenyu Z, Bradford MS, David B, Linlin W, Angel N, Carol W, Christopher R, Lee PL, Jill M, Irena I, Robert D, Alexey B, Michele AC, Hannele RB (2009) MicroRNAs regulate human embryonic stem cell division. Cell Cycle 8:3729-3741. https://doi.org/10.4161/cc.8.22.10033
  9. Kanellopoulou C, Muljo SA, Kung AL, Ganesan S, Drapkin R, Jenuwein T, Livingston DM, Rajewsky K (2005) Dicer-deficient mouse embryonic stem cells are defective in differentiation and centromeric silencing. Genes Dev 19:489-501. https://doi.org/10.1101/gad.1248505
  10. Kathryn N, Deepak S (2010) MicroRNAs as regulators of differentiation and cell fate decisions. Cell Stem Cell 7:36-41. https://doi.org/10.1016/j.stem.2010.06.012
  11. Kato M, Slack FJ (2008) MicroRNAs: small molecules with big roles - C. elegans to human cancer. Biol Cell 100: 71-81. Review. https://doi.org/10.1042/BC20070078
  12. Kim YK, Yu J, Han TS, Park SY, Namkoong B, Kim DH, Hur K, Yoo MW, Lee HJ, Yang HK, Kim VN (2009) Functional links between clustered microRNAs: suppression of cell-cycle inhibitors by microRNA clusters in gastric cancer. Nucleic Acids Res 37:1672-1681. https://doi.org/10.1093/nar/gkp002
  13. Lee SD, Jin JW, Choi J, Choo YK (2009) Ganglioside GT1b mediates neural differentiation of mouse embryonic stem cells. Dev Reprod 13:155-161.
  14. Lin CH, Jackson AL, Guo J, Linsley PS, Eisenman RN (2009) Myc-regulated microRNAs attenuate embryonic stem cell differentiation. EMBO J 28:3157-3170. https://doi.org/10.1038/emboj.2009.254
  15. Marson A, Levine SS, Cole MF, Frampton GM, Brambrink T, Johnstone S, Guenther MG, Johnston WK, Wernig M, Newman J, Calabrese JM, Dennis LM, Volkert TL, Gupta S, Love J, Hannett N, Sharp PA, Bartel DP, Jaenisch R, Young RA (2008) Connecting microRNA genes to the core transcriptional regulatory circuitry of embryonic stem cells. Cell 134:521-533. https://doi.org/10.1016/j.cell.2008.07.020
  16. Murchison EP, Partridge JF, Tam OH, Cheloufi S, Hannon GJ (2005) Characterization of Dicer-deficient murine embryonic stem cells. Proc Natl Acad Sci USA 102:12135-12140. https://doi.org/10.1073/pnas.0505479102
  17. Qi J, Yu JY, Shcherbata HR, Mathieu J, Wang AJ, Seal S, Zhou W, Stadler BM, Bourgin D, Wang L, Nelson A, Ware C, Raymond C, Lim LP, Magnus J, Ivanovska I, Diaz R, Ball A, Cleary MA, Ruohola-Baker H (2009) MicroRNAs regulate human embryonic stem cell division. Cell Cycle 8:3729-3741. https://doi.org/10.4161/cc.8.22.10033
  18. Quintavalle C, Garofalo M, Zanca C, Romano G, Iaboni M, Del Basso De Caro M, Martinez-Montero JC, Incoronato M, Nuovo G, Croce CM, Condorelli G (2011) miR-221/222 overexpression in human glioblastoma increases invasiveness by targeting the protein phosphate PTPu. Oncogene Epub.
  19. Shah MY, Calin GA (2011) MicroRNAs miR-221 and miR-222: a new level of regulation in aggressive breast cancer. Genome Med 3:56. https://doi.org/10.1186/gm272
  20. Silvia G, Neri M, maria GF, Silvia AC (2011) NF-kB and c-Jun induce the expression of the oncogenic miR-221 and miR-222 in prostate carcinoma and glioblastoma cells. Nucleic Acids Research 39:3892-3902. https://doi.org/10.1093/nar/gkr006
  21. Staszel T, Zapała B, Polus A, Sadakierska-Chudy A, Kiec-Wilk B, Stepien E, Wybranska I, Chojnacka M, Dembinska-Kiec A (2011) Role of microRNAs in endothelial cell pathophysiology. Pol Arch Med Wewn 121:361-367.
  22. Stinson S, Lackner MR, Adai AT, Yu N, Kim HJ, O'Brien C, Spoerke J, Jhunjhunwala S, Boyd Z, Januario T, Newman RJ, Yue P, Bourgon R, Modrusan Z, Stern HM, Warming S, de Sauvage FJ, Amier L, Yeh RF, Dornan D (2011) TRPS1 targeting by miR-221/222 promotes the epithelial-to-mesenchymal transition in breast cancer. Sci Signal 4:ra41. https://doi.org/10.1126/scisignal.2001538
  23. Tarantino C, Paolella G, Cozzuto L, Minopoli G, Pastore L, Parisi S, Russo T (2010) miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic stem cells. FASEB J 24:3255-3263. https://doi.org/10.1096/fj.09-152207
  24. Tay Y, Zhang J, Thomson AM, Lim B, Rigoutsos I (2008) MicroRNAs to Nanog, Oct4 and Sox2 coding regions modulate embryonic stem cell differentiation. Nature 4551124-1128.
  25. Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM (1998) Embryonic stem cell lines derived from human blastocysts. Science 282:1145-1147. https://doi.org/10.1126/science.282.5391.1145
  26. Tsunoda T, Takashima Y, Yoshida Y, Doi K, Tanaka Y, Fujimoto T, Machida T, Ota T, Koyanagi M, Kuroki M, Sasazuki T, Shirasawa S (2011) Oncogenic KRAS regulates miR-200c and miR-221/222 in a 3D-specific manner in colorectal cancer cells. Anticancer Res 31:2453-2459.