Korean Journal of Head & Neck Oncology (대한두경부종양학회지)

The Korean Society for Head and Neck Oncology (대한두경부종양학회)
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Identifying Genes Related with Radiation Resistance in Oral Squamous Cell Carcinoma Cell Lines

두경부 편평상피세포암주에서 방사선 내성 관련 유전인자의 확인

  • Lee, Sei-Young (Department of Otolaryngology-Head and Neck Surgery, Chung-Ang University College of Medicine) ;
  • Seol, Jeong-Hun (Department of Otorhinolaryngology, Yonsei University College of Medicine) ;
  • Park, Haeng-Ran (The Yonsei Medical Research Center, Yonsei University College of Medicine) ;
  • Cho, Nam-Hoon (The Yonsei Medical Research Center, Yonsei University College of Medicine) ;
  • Choi, Yoon-Pyo (Department of Pathology, Yonsei University College of Medicine) ;
  • Rha, Sun-Young (Division of Genomics and Translational Research, Cancer Metastasis Research Center, Yonsei University College of Medicine) ;
  • Baek, Seung-Jae (Department of Otorhinolaryngology, Myongji Hospital, Kwandong University College of Medicine) ;
  • Hwang, Jun-Yeon (Department of Otolaryngology-Head and Neck Surgery, Chung-Ang University College of Medicine) ;
  • Kim, Se-Heon (Department of Otorhinolaryngology, Yonsei University College of Medicine)
  • 이세영 (중앙대학교 의과대학 이비인후과학교실) ;
  • 설정훈 (연세대학교 의과대학 이비인후과학교실) ;
  • 박행란 (연세대학교 의과대학 임상의학연구센터) ;
  • 조남훈 (연세대학교 의과대학 임상의학연구센터) ;
  • 최윤표 (연세대학교 의과대학 병리학교실) ;
  • 라선영 (연세대학교 의과대학 암전이연구센터) ;
  • 백승재 (관동대학교 의과대학 명지병원 이비인후과) ;
  • 황준연 (중앙대학교 의과대학 이비인후과학교실) ;
  • 김세헌 (연세대학교 의과대학 이비인후과학교실)
  • Received : 2011.07.28
  • Accepted : 2011.10.11
  • Published : 2011.11.25
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Abstract

Background and Objective : Radiation resistance(RR) is one of main determinants of treatment outcome in oral squamous cell carcinoma(OSCC), but accurate prediction of RR is difficult. We aim to establish RR OSCC cell lines and identify genes related with RR by a measurement of altered gene expression after inducing RR. Material and Methods : OSCC cell lines, SCC15, SCC25 and QLL1, were treated with 2Gy radiation per session, and parts of them were alive in finally accumulated dosage of 60Gy through 30 times repetition of radiotherapy for inducing RR cell lines. We compared results of cDNA array and proteomics in non-radiated cell lines and RR cell lines to detect changes of gene expression. Western blot was used for the validation of results. Results : cDNA array revealed 265 commonly up-regulated genes and 268 commonly down-regulated genes in 3 RR cell lines comparing their non-radiated counterpart. Among them, 30 cancer related genes were obtained. Proteomics showed 51 commonly altered protein expressions in 3 RR cell lines and 18 cancer related proteins were obtained. Among the detected genes, we found NM23-H1 and PA2G4 were over-expressed in both cDNA array and proteomics. Western blot showed increased expression of NME1 in RR cell lines but not in PA2G4. Conclusion: We concluded that NM23-H1 may be a candidate of RR related gene and over-expression of NM23-H1 could be a biomarker to predict RR in OSCC.

Keywords

References

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