Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Biosynthesis of Glycosylated Derivatives of Tylosin in Streptomyces venezuelae

  • Han, Ah-Reum (Interdisciplinary Programs of Bioengineering, Seoul National University) ;
  • Park, Sung-Ryeol (Department of Chemistry and Nano Science, Ewha Womans University) ;
  • Park, Je-Won (Department of Chemistry and Nano Science, Ewha Womans University) ;
  • Lee, Eun-Yeol (Department of Chemical Engineering, College of Engineering, Kyung Hee University) ;
  • Kim, Dong-Myung (Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University) ;
  • Kim, Byung-Gee (Interdisciplinary Programs of Bioengineering, Seoul National University) ;
  • Yoon, Yeo-Joon (Department of Chemistry and Nano Science, Ewha Womans University)
  • Received : 2011.03.02
  • Accepted : 2011.03.23
  • Published : 2011.06.28
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Abstract

Streptomyces venezuelae YJ028, bearing a deletion of the entire biosynthetic gene cluster encoding the pikromycin polyketide synthases and desosamine biosynthetic enzymes, was used as a bioconversion system for combinatorial biosynthesis of glycosylated derivatives of tylosin. Two engineered deoxysugar biosynthetic pathways for the biosynthesis of TDP-3-O-demethyl-D-chalcose or TDP-L-rhamnose in conjunction with the glycosyltransferaseauxiliary protein pair DesVII/DesVIII were expressed in a S. venezuelae YJ028 mutant strain. Supplementation of each mutant strain capable of producing TDP-3-O-demethyl-D-chalcose or TDP-L-rhamnose with tylosin aglycone tylactone resulted in the production of the 3-O-demethyl-D-chalcose, D-quinovose, or L-rhamnose-glycosylated tylactone.

Keywords

References

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