Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Antifungal Effect of Brachyglottis repanda Ethanol Extract

  • Yook, Chan-Nam (Department of Food Science, Wonkwang Health Science) ;
  • Na, Young-Soon (Division of Bionanochemistry, College of Natural Sciences, Wonkwang University) ;
  • Choi, Hwa-Jung (Department of Clinical Pathology, Daejeon Health Science College) ;
  • You, Il-Soo (Department of Nanopolymer, National Chonbuk University) ;
  • Baek, Jong-Min (R&D Center, BRNScience Co. LTD., Seoul National University) ;
  • Baek, Seung-Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • Received : 2010.01.03
  • Accepted : 2010.03.24
  • Published : 2010.06.01
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Abstract

The crude ethanol extract of B. repanda showed the cytotoxic activity against Polio virus (25% activity at $150{\mu}g$/disk) and the minor cytotoxic activity against BSC cells (African green monkey kidney). However, the crude ethanol extract of B. repanda was non-toxic to murine leukaemia cells CCL 46 P388D1 ($IC_{50}$, > 62,500 ng/ml). Cytotoxic and antifungal activities were strongly shown by Fr. 64-3 which was eluted with 90% $CH_3CN/H_2O$, 100% $CH_3CN$, and 50% $CH_3CN/H_2O$(SM 2 at $150{\mu}g$/disk). The fraction 64-3 also showed the most cytotoxic activity against murine leukaemia cells (128 mg, $IC_{50}$ 10,051 ng/ml at $75{\mu}g$/disk). These results suggest that this fraction has a potent antifungal activity against the dermatophytic fungus Trichophyton mentagrophytes ATCC 28185.

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References

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