Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Effect of DHU001, a Polyherbal Formula, on Dinitrofluorobenzene-induced Contact Dermatitis (Type I allergy)

  • Lee, Hyeung-Sik (Department of Clinical Laboratory Science, College of Health and Therapy, Daegu Haany University) ;
  • Lee, Byung-Chang (Development Anatomy and Histology, College of Oriental Medicine, Daegu Haany University) ;
  • Ku, Sae-Kwang (Development Anatomy and Histology, College of Oriental Medicine, Daegu Haany University)
  • Received : 2010.02.16
  • Accepted : 2010.05.10
  • Published : 2010.06.01
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Abstract

The effect of DHU001, a mixed herbal formula consisted of 7 types aqueous extracts for various respiratory disorders were evaluated on 2,4-dinitrofluorobenzene (DNFB)-induced contact dermatitis, type I allergic model. Contact dermatitis was induced by sensitization with dinitrophenyl-derivatized ovalbumin (DNP-OVA) and DNFB challenge as antigen. Two different dosages of DHU001 (300 and 150 mg/kg) were orally administered to DNP-OVA sensitization mice once a day for 7 days with reference material, dexamethasone (15 mg/kg, intraperitoneal treatment). End of 7 days oral administration of DHU001 extracts or intraperitoneal treatment of dexamethasone, the changes on the edematous changes and scratching behavior were measured. Immediate after DNFB challenge on ear or paw of DNP-OVA sensitized mice, increases of ear and paw thicknesses and weights were detected with anterior ear skin (dermis to epidermis) thickness and paw scratching behavior increases. However, these contact dermatitis signs induced by DNFB treatment were reduced by treatment of the both different dosages of DHU001 and dexamethasone, respectively. The results obtained in this study suggest that oral treatment of DHU001 extracts also has relatively favorable effects on contact dermatitis.

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References

  1. Ahn, K.S., Noh, E.J., Zhao, H.L., Jung, S.H., Kang, S.S. and Kim, Y.S. (2005). Inhibition of inducible nitric oxide synthase and cyclooxygenase II by Platycodon grandiflorum saponins via suppression of nuclear factor-kappaB activation in RAW 264.7 cells. Life Sci., 76, 2315-2328. https://doi.org/10.1016/j.lfs.2004.10.042
  2. Aimbire, F., Penna, S.C., Rodrigues, M., Rodrigues, K.C., Lopes-Martins, R.A. and Sertie, J.A. (2007). Effect of hydroalcoholic extract of Zingiber officinalis rhizomes on LPS-induced rat airway hyperreactivity and lung inflammation. Prostaglandins Leukot Essent Fatty Acids, 77, 129-138. https://doi.org/10.1016/j.plefa.2007.08.008
  3. Amin, A.R., Vyas, P., Attur, M., Leszczynska-Piziak, J., Patel, I.R., Weissmann, G. and Abramson, S.B. (1995). The mode of action of aspirin-like drugs: effect on inducible nitric oxide synthase. Proc. Natl. Acad. Sci. USA, 92, 7926-7930. https://doi.org/10.1073/pnas.92.17.7926
  4. Back, Y.D., Lee, H.S. and Ku, S.K. (2008). Effects of DHU001, a mixed herbal formula on acute inflammation in mice. Toxicol. Res., 24, 189-194. https://doi.org/10.5487/TR.2008.24.3.189
  5. Baliga, M.S., Jagetia, G.C., Rao, S.K. and Babu, K. (2003). Evaluation of nitric oxide scavenging activity of certain spices in vitro: a preliminary study. Nahrung, 47, 261-264. https://doi.org/10.1002/food.200390061
  6. Eisen, H.N. and Belmam, S. (1953). Studies of hypersensitivity to low molecular weight substances. II. Reactions of some allergenic substituted dinitrobenzenes with cysteine or cystine of skin proteins. J. Exp. Med., 98, 533-549. https://doi.org/10.1084/jem.98.6.533
  7. Foroughi, S., Thyagarajan, A. and Stone, K.D. (2005). Advances in pediatric asthma and atopic dermatitis. Curr. Opin. Pediatr., 17, 658-663. https://doi.org/10.1097/01.mop.0000178386.61932.0b
  8. Ganir, E.M., Capulong, M.C., Tahara, K., Akasawa, A. and Iikura, Y. (1996). Treatment of atopic dermatitis in children: the importance of skin care and environmental control. Acta Paediatr. Jpn., 38, 702-704. https://doi.org/10.1111/j.1442-200X.1996.tb03737.x
  9. Geronikaki, A.A. and Gavalas, A.M. (2006). Antioxidants and inflammatory disease: synthetic and natural antioxidants with anti-inflammatory activity. Comb. Chem. High Throughput Screen, 9, 425-442. https://doi.org/10.2174/138620706777698481
  10. Ghayur, M.N., Gilani, A.H. and Janssen, L.J. (2008). Ginger attenuates acetylcholine-induced contraction and Ca2+ signalling in murine airway smooth muscle cells. Can. J. Physiol. Pharmacol., 86, 264-271. https://doi.org/10.1139/Y08-030
  11. Gordon, J.S., Wolanin, P.M., Gonzalez, A.V., Fela, D.A., Sarngadharan, G., Rouzard, K., Perez, E., Stock, J.B. and Stock, M.B. (2008). Topical N-acetyl-S-farnesyl-L-cysteine inhibits mouse skin inflammation, and unlike dexamethasone, its effects are restricted to the application site. J. Invest. Dermatol., 128, 643-654.
  12. Gupta, A.K. and Chow, M. (2004). Prednicarbate (Dermatop): profile of a corticosteroid. J. Cutan. Med. Surg., 8, 244-247. https://doi.org/10.1007/s10227-004-0120-x
  13. Han, B.H., Yoo, S.Y., Park, M.H. and Lee, H.J. (1981). Antioxidant activity screening on crude drugs. J. Pharmacogn., 12, 66.
  14. Ishiguro, K., Oku, H., Suitani, A. and Yamamoto, Y. (2002). Effects of conjugated linoleic acid on anaphylaxis and allergic pruritus. Biol. Pharm. Bull., 25, 1655-1657. https://doi.org/10.1248/bpb.25.1655
  15. Kiczuk, K., Chodorowska, G. and Krasowska, D. (2004). The care of the child with atopic dermatitis. Ann. Univ. Mariae Curie Sklodowska [Med.], 59, 503-507.
  16. Kim, S.S., Lee, S.C., Shin, H.D., Shin, M.K., Kim, J.H. and Song, H.J. (2004). Studies on allergy asthma effect of Radix Platicodi. Kor. J. Herbology, 19, 61-70.
  17. Klecz, R.J. and Schwartz, R.A. (1992). Pruritus. Am Family Physician, 45, 2681-2686.
  18. Ko, K.M. and Lam, B.Y. (2002). Schisandrin B protects against tert-butylhydroperoxide induced cerebral toxicity by enhancing glutathione antioxidant status in mouse brain. Mol. Cell Biochem., 238, 181-186. https://doi.org/10.1023/A:1019907316129
  19. Lorette, G. and Vaillant, L. (1990). Pruritus: current concepts in pathogenesis and treatment. Drugs, 39, 218-223. https://doi.org/10.2165/00003495-199039020-00005
  20. Masuda, Y., Kikuzaki, H., Hisamoto, M. and Nakatani, N. (2004). Antioxidant properties of gingerol related compounds from ginger. Biofactors, 21, 293-296. https://doi.org/10.1002/biof.552210157
  21. Matsuda, H., Tomohiro, N., Ido, Y. and Kubo, M. (2002). Antiallergic effects of cnidii monnieri fructus (dried fruits of Cnidium monnieri) and its major component, osthol. Biol. Pharm. Bull., 25, 809-812. https://doi.org/10.1248/bpb.25.809
  22. Matsumoto, K., Mizukoshi, K., Oyobikawa, M., Ohshima, H., Sakai, Y. and Tagami, H. (2005). Objective evaluation of the efficacy of daily topical applications of cosmetics bases using the hairless mouse model of atopic dermatitis. Skin Res. Technol., 11, 209-217. https://doi.org/10.1111/j.1600-0846.2005.00106.x
  23. Minghetti, P., Sosa, S., Cilurzo, F., Casiraghi, A., Alberti, E., Tubaro, A., Loggia, R.D. and Montanari, L. (2007). Evaluation of the topical anti-inflammatory activity of ginger dry extracts from solutions and plasters. Planta Med., 73, 1525-1530. https://doi.org/10.1055/s-2007-993741
  24. Nagai, H., Hiyama, H., Matsuo, A., Ueda, Y., Inagaki, N. and Kawada, K. (1997a). FK-506 and cyclosporin A potentiate the IgE antibody production by contact sensitization with hapten in mice. J. Pharmacol. Exp. Ther., 283, 321-327.
  25. Nagai, H., Matsuo, A., Hiyama, H., Inagaki, N. and Kawada, K. (1997b). Immunoglobulin E production in mice by means of contact sensitization with a simple chemical, hapten. J. Allergy Clin. Immunol., 100, S39-44. https://doi.org/10.1016/S0091-6749(97)70003-4
  26. Nakahara, T. and Furue, M. (2005). Mechanism of pathogenesis in atopic dermatitis. Nippon Rinsho, 63, 80-85.
  27. Narimanian, M., Badalyan, M., Panosyan, V., Gabrielyan, E., Panossian, A., Wikman, G. and Wagner, H. (2005). Impact of Chisan (ADAPT-232) on the quality-of-life and its efficacy as an adjuvant in the treatment of acute non-specific pneumonia. Phytomedicine, 12, 723-729. https://doi.org/10.1016/j.phymed.2004.11.004
  28. Ohtsuka, E., Kawai, S., Nojima, H., Andoh, T., Kamimura, K. and Kuraishi, Y. (2003). Inhibitory effect of azelastine on allergic itch-associated response in mice sensitized with mosquito salivary glands extract. J. Pharmacol. Sci., 91, 263-266. https://doi.org/10.1254/jphs.91.263
  29. Park, E., Kum, S., Wang, C., Park, S.Y., Kim, B.S. and Schuller-Levis, G. (2005). Anti-inflammatory activity of herbal medicines: inhibition of nitric oxide production and tumor necrosis factor-alpha secretion in an activated macrophage-like cell line. Am. J. Chin. Med., 33, 415-424. https://doi.org/10.1142/S0192415X05003028
  30. Park, J.H. and Geon, D.G. (2003). Pharmacognostical studies on the Chinese crude drug “Maig Moon Dong”. Kor. J. Pharmacogn., 34, 6-9.
  31. Perez, A., Narayan, S. and Sansom, J. (2004). Occupational contact dermatitis from 2,4-dinitrofluorobenzene. Contact Dermatitis, 51, 314.
  32. Perez, C., Canal, J.R. and Torres, M.D. (2003). Experimental diabetes treated with Ficus carica extract: effect on oxidative stress parameters. Acta Diabetol., 40, 3-8. https://doi.org/10.1007/s005920300001
  33. Ramprasath, V.R., Shanthi, P. and Sachdanandam, P. (2006). Immunomodulatory and anti-inflammatory effects of Semecarpus anacardium LINN. Nut milk extract in experimental inflammatory conditions. Biol. Pharm. Bull., 29, 693-700. https://doi.org/10.1248/bpb.29.693
  34. Rhyu, M.R., Kim, E.Y., Yoon, B.K., Lee, Y.J. and Chen, S.N. (2006). Aqueous extract of Schizandra chinensis fruit causes endothelium-dependent and -independent relaxation of isolated rat thoracic aorta. Phytomedicine, 13, 651-657. https://doi.org/10.1016/j.phymed.2006.02.003
  35. Rossetti, G., Laffitte, E., Eigenmann, P.A., Lubbe, J., Hohl, D. and Hofer, M.F. (2005). Treatment of atopic dermatitis: practical approach. Rev. Med. Suisse, 1, 501-504.
  36. Shichinohe, K., Shimizu, M. and Kurokawa, K. (1996). Effect of M-711 on experimental skin reactions induced by chemical mediators in rats. J. Vet. Med. Sci., 58, 419-423. https://doi.org/10.1292/jvms.58.419
  37. Shin, T.Y. (2003). Inhibition of immunologic and nonimmunologic stimulation-mediated anaphylactic reactions by the aqueous extract of Mentha arvensis. Immunopharmacol. Immunotoxicol., 25, 273-283.
  38. Skinner, R. (2004). Role of topical therapies in the management of cutaneous disease. J. Cutan. Med. Surg., 8, 22-31. https://doi.org/10.1007/s10227-004-0805-1
  39. Steenvoorden, D.P. and Beijersbergen van Henegouwen, G. (1999). Protection against UV-induced systemic immunosuppression in mice by a single topical application of the antioxidant vitamins C and E. Int. J. Radiat. Biol., 75, 747-755. https://doi.org/10.1080/095530099140096
  40. Sun, H.X. and Pan, H.J. (2006). Immunological adjuvant effect of Glycyrrhiza uralensis saponins on the immune responses to ovalbumin in mice. Vaccine, 24, 1914-1920. https://doi.org/10.1016/j.vaccine.2005.10.040
  41. Tan, B.K. and Vanitha, J. (2004). Immunomodulatory and antimicrobial effects of some traditional Chinese medicinal herbs: a review. Curr. Med. Chem., 11, 1423-1430. https://doi.org/10.2174/0929867043365161
  42. Wang, C., Schuller Levis, G.B., Lee, E.B., Levis, W.R., Lee, D.W., Kim, B.S., Park, S.Y. and Park, E. (2004). Platycodin D and D3 isolated from the root of Platycodon grandiflorum modulate the production of nitric oxide and secretion of TNF-alpha in activated RAW 264.7 cells. Int. Immunopharmacol., 4, 1039-1049. https://doi.org/10.1016/j.intimp.2004.04.005
  43. Wang, H.W., Tedla, N., Lloyd, A.R., Wakefield, D. and McNeil, P.H. (1998). Mast cell activation and migration to lymph nodes during induction of an immune response in mice. J. Clin. Invest., 102, 1617-1626. https://doi.org/10.1172/JCI3704
  44. Watanabe, C., Satoh, T., Tahara, E., Murakami, K., Hayashi, K., Hase, K., Andoh, T., Kuraishi, Y., Kadota, S., Nagai, H. and Saiki, I. (1999). Inhibitory mechanisms of glycoprotein fraction derived from Miscanthus sinensis for the immediate phase response of an IgE-mediated cutaneous reaction. Biol. Pharm.Bull., 22, 26-30. https://doi.org/10.1248/bpb.22.26
  45. Williams, H.C. (2005). Clinical practice. Atopic dermatitis. N. Engl. J. Med., 352, 2314-2324. https://doi.org/10.1056/NEJMcp042803
  46. Wright, R.J., Cohen, R.T. and Cohen, S. (2005). The impact of stress on the development and expression of atopy. Curr. Opin. Allergy Clin. Immunol., 5, 23-29. https://doi.org/10.1097/00130832-200502000-00006
  47. Ueda, Y., Sone, T., Inagaki, N. and Nagai, H. (2003). Effects of prednisolone on the cutaneous reaction and skin barrier function in mice treated with a hapten. Biol. Pharm. Bull., 26, 618-621. https://doi.org/10.1248/bpb.26.618
  48. Yoon, Y.D., Han, S.B., Kang, J.S., Lee, C.W., Park, S.K., Lee, H.S., Kang, J.S. and Kim, H.M. (2003). Toll-like receptor 4-dependent activation of macrophages by polysaccharide isolated from the radix of Platycodon grandiflorum. Int. Immunopharmacol., 3, 1873-1882. https://doi.org/10.1016/j.intimp.2003.09.005
  49. Zhou, Y. and Xu, R. (1992). Antioxidative effect of Chinese drugs. Zhongguo Zhong Yao Za Zhi, 17, 368-369, 373.

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