The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지

Anti-inflammatory Effects of Sam-chul-kun-bi-tang

  • Lee, Jin-Ah (Herbal Medicine Evidence-Based Medicine Research Center, Korea Institute of Oriental Medicine) ;
  • Ha, Hye-Kyung (Herbal Medicine Evidence-Based Medicine Research Center, Korea Institute of Oriental Medicine) ;
  • Jung, Da-Young (Herbal Medicine Evidence-Based Medicine Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Ho-Young (Herbal Medicine Evidence-Based Medicine Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Nam-Hun (Herbal Medicine Evidence-Based Medicine Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Jun-Kyoung (Herbal Medicine Evidence-Based Medicine Research Center, Korea Institute of Oriental Medicine) ;
  • Huang, Dae-Sun (Herbal Medicine Evidence-Based Medicine Research Center, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (Herbal Medicine Evidence-Based Medicine Research Center, Korea Institute of Oriental Medicine)
  • Received : 2010.02.17
  • Accepted : 2010.04.22
  • Published : 2010.05.30
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Abstract

Objective: To derive information on the efficacy of Sam-chul-kun-bi-tang (SKT), by evaluating its anti-inflammatory effect. SKT is a widely-used herbal formula in traditional Korean medicine. In man y studies, plant-derived anti-inflammatory efficacies have been investigated for their potential inhibitory effects on lipopolysaccharide (LPS)-stimulated macrophages. This study was performed to examine the anti-inflammatory effects of SKT extract on LPS-stimulated RAW 264.7 cells. Methods: The production of nitric oxide (NO), prostaglandin $(PG)E_2$, tumor necrosis factor (TNF)-$\alpha$ and interleukin (IL)-6 were examined in a macrophage cell line, RAW 264.7 cells, in the presence of SKT. RAW 264.7 cells were incubated with LPS 1 ${\mu}g/mL$ and SKT for 18 hrs. The anti-inflammatory activity of SKT was investigated by carrageenan-induced paw edema in rats. The paw volume was measured at 2 and 4 hrs following carrageenan-induced paw edema in rats. Results: SKT showed inhibitory effect on $PGE_2$, TNF-$\alpha$ and IL-6 in LPS-stimulated RAW 264.7 cells. But SKT was not inhibitory effect on NO by LPS-stimulated RAW 264.7 cells. Administration of SKT (1 g/kg) also showed a reduction in carrageenan-induced paw edema on rats. Conclusion: These results suggest that SKT has anti-inflammatory activities in both in vitro and in vivo models.

Keywords

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