The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지

Antitumor Effect of Hang-Am-Dan Non-boiled Water Extracts on NCI-H460 Tumor Regression Model

  • Kim, Jun-Lae (East-West Cancer Center, Dunsan Oriental Hospital of Daejeon University) ;
  • Kim, Kyung-Soon (East-West Cancer Center, Dunsan Oriental Hospital of Daejeon University) ;
  • Park, Jae-Woo (East-West Cancer Center, Dunsan Oriental Hospital of Daejeon University) ;
  • Lee, Yeon-Weol (East-West Cancer Center, Dunsan Oriental Hospital of Daejeon University) ;
  • Cho, Chong-Kwan (East-West Cancer Center, Dunsan Oriental Hospital of Daejeon University) ;
  • Yoo, Hwa-Seung (East-West Cancer Center, Dunsan Oriental Hospital of Daejeon University)
  • Received : 2010.02.17
  • Accepted : 2010.05.03
  • Published : 2010.05.30
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Abstract

Objective: This experimental study was performed to examine if Hang-Am-Dan non-boiled water extracts (HAD-N) induce apoptosis in human lung carcinoma NCI-H460 cells in vitro and inhibits the growth of NCI-H460 cell-transplanted solid tumor in vivo. Materials and Methods: We cultured NCI-H460 cell lines and xenografted them to nude mice. The mice were divided into 3 groups, NCI-H460 cell alone, NCI-H460 + 90 mg/kg HAD-N treated group, and NCI-H460 + 180 mg/kg HAD-N treated group, with seven mice per group. HAD-N was orally administrated every day for four weeks. We checked their body weight and tumor weight and volumes two times a week and their absolute organ weight and biochemical blood analysis at the final day by sacrificing them. We also calculated their tumor inhibition rate (IR), mean survival time and percent increase in life span (% ILS). Results: In this study, we observed that all of the HAD-N treated mice got smaller tumors. The more doses of HAD-N used, the less IR showed at the 8th day after starting this experiment. Tumor weight and volume of HAD-N treatment groups also decreased. Mean survival time and percent increase in life span (% ILS) in the high-dose HAD-N treatment groups were higher than those of other groups. The test substances in the blood level UN results showed reduction in the significance in both HAD-N 90 mg/kg and HAD-N 180 mg/kg (p<0.01). The blood level phosphatase results in HAD-N 90 mg/kg group compared to NCI-H460 cell alone group showed a reduction in significance (p<0.05). AST levels HAD-N 180 mg/kg group compared to NCI-H460 cell alone group significance as well (p<0.05). Conclusion: We suggest that the results of the in vivo study showed that HAD-N may have potential as a growth inhibitor of tumor-induced NCI-H460 of nude mice in spite of the shortcomings of this study. More studies to overcome those shortcomings and to find out significant antitumor mechanism will be needed.

Keywords

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