Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Inhibitory Effect of Heartwood of Rhus verniciflua Stokes on Lipid Accumulation in 3T3-L1 Cells

3T3-L1 세포에 대한 옻나무 추출물의 지방축적 억제효과

  • Kim, Se-Gun (Department of Oriental Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Rhyu, Dong-Young (Department of Medicinal Plant Resources, Mokpo National University) ;
  • Kim, Do-Kuk (Department of Oriental Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Ko, Da-Hyung (Department of Oriental Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Kim, Yun-Kyung (Department of Oriental Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Lee, Young-Mi (Department of Oriental Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Jung, Hyun-Ju (Department of Oriental Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University)
  • 김세건 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 류동영 (목포대학교 한약자원학과) ;
  • 김도국 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 고다형 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 김윤경 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 이영미 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 정현주 (원광대학교 약학대학 한약학과 및 원광한약연구소)
  • Received : 2010.01.23
  • Published : 2010.03.31
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Abstract

The MeOH extract of Rhus verniciflua heartwood inhibited lipid accumulation in 3T3-L1 adipocytes. Chromatographic methods including of silica gel, RP-18 and high-pressure liquid chromatography isolated sulfuretin and fisetin from the extract as active constituents. The isolated compounds, especially sulfuretin, strongly inhibited lipid accumulation in adipocytes. The treatment of sulfuretin and fisetin led to decreased expression of peroxisome proliferator-activated receptor-gamma ($PPAR{\gamma}$), as an important transcription factor in fat cell differentiation, which was equal to the decrease in the quercetin positive control. The presence of a hydroxyl group (C-5) in quercetin compared to fisetin, and the presence of C-2 double bonds in fisetin compared with fustin increased the inhibitory effect of lipid accumulation.

Keywords

References

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