Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Inhibition of LPS-induced iNOS, COX-2 Expression and Cytokines Production by Fupenjic Acid in Macrophage Cells

Fupenjic Acid의 대식세포에서 LPS에 의해 유도되는 iNOS와 COX-2 발현 및 Cytokine들의 생성 저해 효과

  • Yun, Chang-Hyeon (Department of pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University) ;
  • Shin, Ji-Sun (Department of pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University) ;
  • Park, Hee-Juhn (Division of Environmental Botany, Sangji University) ;
  • Park, Jong-Hee (College of Pharmacy, Pusan National University) ;
  • Lee, Kyung-Tae (Department of pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University)
  • Received : 2010.01.06
  • Published : 2010.03.31
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Abstract

In this study, we investigated the anti-inflammatory effects of fupenjic acid (FA) isolated from the Potentilla discolor in both RAW 264.7 and mouse primary peritoneal macrophage cells. FA pretreatment significantly inhibited nitric oxide (NO) and prostaglandin $E_2(PGE_2)$ productions in the lipopolysaccharide (LPS)-induced RAW 264.7 and mouse primary peritoneal macrophage cells. Consistent with these observations, Western blot and RT-PCR analyses revealed that FA inhibited the LPS-induced expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the protein and mRNA levels. In addition, FA reduced the release of tumor necrosis factor-$\alpha$ (TNF-$\alpha$) and interleukin-6 (IL-6). These results suggest that the down regulation of iNOS and COX-2 expression and TNF-$\alpha$ and IL-6 production by fupenjic acid are responsible for its anti-inflammatory effects.

Keywords

References

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