Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Antibacterial Effect of Oriental Medicinal Herbs on Dental Pathogens

구강질환 세균에 대한 한약재의 항균효과

  • Yu, Young-Eun (The Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University) ;
  • Park, Eun-Young (The Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University) ;
  • Jung, Dae-Hwa (The Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University) ;
  • Byun, Sung-Hui (College of Oriental Medicine, Daegu Haany University) ;
  • Kim, Sang-Chan (The Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University) ;
  • Park, Sung-Min (The Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University)
  • 유영은 (대구한의대학교 한방생명자원연구센터) ;
  • 박은영 (대구한의대학교 한방생명자원연구센터) ;
  • 정대화 (대구한의대학교 한방생명자원연구센터) ;
  • 변성희 (대구한의대학교 한의학과) ;
  • 김상찬 (대구한의대학교 한방생명자원연구센터) ;
  • 박성민 (대구한의대학교 한방생명자원연구센터)
  • Received : 2010.03.02
  • Accepted : 2010.04.19
  • Published : 2010.06.30
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Abstract

In this study, we investigated the antibacterial effect of 69 oriental medicinal herbs (OMHs) on Actinomyces viscocus, Streptococcus sobrinus, and two strains of Streptococcus mutans as oral bacteria. Methanol extracts of OMHs were used in the first antibacterial test, and then boiled water, ethanol, methanol, and ethyl acetate extracts of C. japonica, C. sappan, P. mume, and S. chinensis were used in the second test. Boiled water extract of C. japonica was shown to have the most superior effect on oral bacteria. The extract yield of boiled water extract of C. japonica was 22%, and the growth of oral bacteria was almost inhibited at over a 180 mg/ml concentration. Antibacterial effect compound analyzed by UPLC method was identified as berberine, and the content was 25.54%. In addition, the extract appeared to be stable at $121^{\circ}C$ for 15 min.

본 연구에서는 69가지의 한약재를 이용하여 구강세균인 Actinomyces viscocus, Streptococcus sobrinus, 그리고 Streptococcus mutans 2종에 대한 항균효과를 조사하였다. 우선 선발된 한약재를 메탄올로 추출하여 조사하였으며 그 결과를 바탕으로 소목, 오매, 오미자, 그리고 황련을 선별하여 열수, 에탄올, 메탄올, 그리고 에틸아세테이트를 이용하여 항균효과를 조사하였다. 조사결과 황련 열수 추출물이 가장 우수한 효과를 나타내는 것으로 조사되었다. 황련 열수 추출물을 이용하여 공시균주에 대한 저해양상을 조사한 결과 180 mg/ml 이상의 농도에서 공시균주의 생육이 대부분 저해되는 것을 확인할 수 있었으며 이 때 황련 열수 추출물의 회수율은 22%였다. 황련 열수 추출물을 UPLC를 이용하여 berberine 함량을 조사한 결과 25.54%로 조사되었다. 또한 $121^{\circ}C$에서 15분간 열 처리 후 황련 열수 추출물의 열 안정성을 조사한 결과 열에 대하여 안정 하다는 것을 확인할 수 있었다.

Keywords

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