Asian-Australasian Journal of Animal Sciences

  • 제23권10호
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  • Pages.1276-1281
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  • 2010
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Effects of Butylated Hydroxytoluene on Freezability of Ram Spermatozoa

  • Farshad, A. (Department of Animal Science, College of Agriculture, University of Kurdistan) ;
  • Khalili, B. (Jafar-Abad Livestock Central Research Institute, Ministry of Agriculture) ;
  • Jafaroghli, M. (College of Agriculture, University of Payam-e-Noor)
  • 투고 : 2009.11.23
  • 심사 : 2010.03.25
  • 발행 : 2010.10.01
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초록

The present study was conducted to investigate the effects of butylated hydroxytoluene (BHT) supplementation on diluted, cooled and frozen-thawed ram spermatozoa. After primary evaluation of collected ejaculates, only semen samples with motility of more than 70% and sperm concentration higher than $3{\times}10^3$ sperm/ml were used for cryopreservation. The selected semen samples were then pooled and diluted 1:4 with Tris Citrate Fructose Yolk (TCFY) extender supplemented with different concentrations of BHT (0.5, 10, 2.0 and 3.0 mM). As the control, semen was diluted and frozen in the diluent without BHT. Motility, progressive motility, viability, membranes and acrosome integrity were evaluated after dilution (part 1), cooling (part 2) and freezing and thawing (part 3). The results of the first part of the experiment showed that there were no significant difference between treatments in the motility, progressive motility, viability, membranes and acrosome integrity of spermatozoa, but the results with 2.0 mM BHT were slightly better than obtained with other levels of BHT and control extender. Significantly better results (p<0.05) were observed in the second part of the experiment for cooled spermatozoa characteristics, when extender was supplemented with 2.0 and 3.0 mM BHT. Furthermore, the results obtained in the third part of the experiment indicated that, after freezing and thawing, all evaluated semen characteristics were improved significantly (p<0.05) by increasing BHT levels, with the best results obtained for extender containing 2 mM BHT. Comparison of these results with those of control diluent, the effects of supplementation were significantly (p<0.01) better. However, the higher concentration of BHT (3.0 mM) reduced the motility, acrosomal integrity, viability and hypo-osmotic swelling response of spermatozoa compared to extender containing 2.0 mM BHT. In conclusion, the results obtained in this study showed that the semen quality of rams was improved when BHT was added to extender used before the freezing process.

키워드

참고문헌

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피인용 문헌

  1. Protective effect of butylated hydroxytoluene on sperm function in human spermatozoa cryopreserved by vitrification technique vol.47, pp.2, 2014, https://doi.org/10.1111/and.12246
  2. Effect of supplementation of butylated hydroxytoluene on post-thaw sperm viability, motility and membrane integrity of Hariana bulls vol.8, pp.6, 2015, https://doi.org/10.14202/vetworld.2015.808-812
  3. Soy lecithin as a potential alternative to powdered egg yolk for buck sperm cryopreservation does not protect them from mitochondrial damage vol.217, pp.None, 2010, https://doi.org/10.1016/j.anireprosci.2020.106473
  4. Effects of astaxanthin supplementation on the freezability, lipid peroxidation, antioxidant enzyme activities and post-thawing fertility of ram semen vol.192, pp.None, 2010, https://doi.org/10.1016/j.smallrumres.2020.106213