Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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Identification of Quantitative Trait Loci Associated with Anthracnose Resistance in Chili Pepper (Capsicum spp.)

고추 탄저병 저항성 관련 양적형질 유전자좌 분석

  • Kim, Su (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science) ;
  • Kim, Ki-Taek (The Foundation of Agri. Technology Commercialization and Transfer) ;
  • Kim, Dong-Hwi (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science) ;
  • Yang, Eun-Young (Vegetable Research Division, National Institute of Horticultural & Herbal Science) ;
  • Cho, Myeong-Cheoul (Vegetable Research Division, National Institute of Horticultural & Herbal Science) ;
  • Jamal, Arshad (College of Science & Arts Rabaigh Campus, King Abdullaziz University) ;
  • Chae, Young (Technology Services Division, National Institute of Horticultural & Herbal Science) ;
  • Pae, Do-Ham (The Foundation of Agri. Technology Commercialization and Transfer) ;
  • Oh, Dae-Geun (Korea National College of Agriculutre and Fisheries) ;
  • Hwang, Ju-Kwang (Department of Horticultural Science, Chungbuk National University)
  • 김수 (국립원예특작과학원 원예특작환경과) ;
  • 김기택 (농업기술실용화재단) ;
  • 김동휘 (국립원예특작과학원 원예특작환경과) ;
  • 양은영 (국립원예특작과학원 채소과) ;
  • 조명철 (국립원예특작과학원 채소과) ;
  • ;
  • 채영 (국립원예특작과학원 기술지원과) ;
  • 배도함 (농업기술실용화재단) ;
  • 오대근 (한국농수산대학교) ;
  • 황주광 (충북대학교 원예과학과)
  • Received : 2010.06.13
  • Accepted : 2010.10.13
  • Published : 2010.12.31
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Abstract

Pepper ($Capsicum$ spp.) anthracnose caused by $Colletotrichum$ $acutatum$ is a destructive disease susceptible to areas where chili peppers are grown. $Capsicum$ $baccatum$ var. $pendulum$ (Cbp) is resistant to anthracnose and has actively been used for interspecific hybridization for the introgression of resistance gene(s) into cultivated chili peppers. The goals of this study were to determine the inheritance of resistance to anthracnose within $Capsicum$ $baccatum$ and to map quantitative trait loci (QTLs) for the anthracnose resistance. A genetic mapping population consisting of 126 $F_2$ plants derived from a cross between $Capsicum$ $baccatum$ var. $pendulum$ (resistant) and $Capsicum$ $baccatum$ 'Golden-aji' (susceptible) was used for linkage mapping. The linkage map was constructed with 52 SSRs, 175 AFLPs, and 100 SRAPs covering 1,896cM, with an average interval marker distance of 4.0cM. Based on this map, the number, location, and effect of QTLs for anthracnose resistance were studied using plants inoculated in the laboratory and field. A total of 19 quantitative trait loci (2 major QTLs and 16 minor QTLs) were detected. Two QTLs ($An8.1$, $An9.1$) showed 16.4% phenotypic variations for anthracnose resistance after wounding inoculation. In addition, five minor QTL loci ($An7.3$, $An7.4$, $An4.1$, $An3.1$, $An3.2$) showed a total of 60.73% phenotypic variations of anthracnose resistance in the field test. Several significant QTLs were also detected and their reproducibility was confirmed under different inoculation conditions. These QTLs are now being confirmed with different breeding populations. Markers tightly linked to the QTLs that are reliable under different environmental conditions will help to determine the success of marker-assisted selection for anthracnose -resistant breeding programs in chili pepper.

고추 탄저병은 국내외에서 고추 생산에 있어 많은 피해를 주는 병중의 하나로서, $Colletotrichum$ spp.에 의해 발생하며 현재 가장 많은 빈도를 나타내는 종은 $C.$ $acutatum$으로 보고되고 있다. 살균제에 대한 내성이 있는 것으로 보고되어 저항성 품종의 육성이 요구되어지고 있다. 본 연구의 목적은 $Capsicum$ $baccatum$ 종내 교잡을 통해 고추 탄저병 저항성 육종을 위한 MAS(marker assisted selection) 체계를 개발하는 기초연구로서 저항성과 관련한 QTL분석을 수행하였다. 저항성 검정결과 고추 탄저병저항성은 85%의 높은 광의의 유전력을 보였다. 양적형질 유전자좌 분석 결과 신뢰수준 이상의 LOD 수준을 보인 2개의 QTL($An8.1$, $An9.1$)이 탐색되었고, $R^2$가 2.04%와 14.36%이었다. 유전적인 효과는 $An8.1$은 상가적 효과가 0.46으로, $An9.1$은 상가적 효과가 -4.52로 나타났다. $An8.1$과 $An9.1$은 상처접종에 의해 나타나는 저항성 반응과 관련이 있는 것으로 생각되었다. 포장검정(08NR)과 관련한 QTL들은 신뢰수준이상의 LOD수준을 보이는 QTL은 탐색할 수 없었으나, $R^2$가 19.9%인 $An3.1$와 30.8%인 $An3.1$을 포함한 5개 QTL의 전체 $R^2$가 60.73%로 나타나 고추 탄저병저항성 유전과 관련한 인자가 다수 존재하는 것을 확인하였다. 따라서 이들 QTL은 더욱 정밀한 집단과 종간교잡 후대에서 적용성을 검정하여 고추 탄저병저항성 품종육성을 위한 MAS체계를 확립할 것이다.

Keywords

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