Genetic Relationship of Genus Rosa Germplasm and Genetic Diversity of Rosa rugosa in Korea

장미속 유전자원의 유전적 관계와 국내 해당화의 유전적 다양성

  • Joung, Youn-Hwa (Department of Plant Biotechnology, Chonnam National University) ;
  • Kim, Seung-Tae (Floriculture Research Division, National Institute of Horticultural & Herbal Science) ;
  • Kim, Gi-Jun (Breeding Institution, Asia Seed Co. Ltd.) ;
  • Lee, Ja-Hyun (Department of Plant Biotechnology, Chonnam National University) ;
  • Gi, Gwang-Yeon (Horticultural Research Division, Jeollam-do Agricultural Research & Extension Services) ;
  • Han, Tae-Ho (Department of Plant Biotechnology, Chonnam National University)
  • 정연화 (전남대학교 식물생명공학과) ;
  • 김성태 (국립원예특작과학원 화훼과) ;
  • 김기준 (아시아종묘 육종연구소) ;
  • 이자현 (전남대학교 식물생명공학과) ;
  • 기광연 (전라남도농업기술원 원예연구소) ;
  • 한태호 (전남대학교 식물생명공학과)
  • Received : 2010.05.13
  • Accepted : 2010.10.13
  • Published : 2010.12.31

Abstract

Fifty-nine accessions of 23 species in genus Rosa were collected, and 15 accessions of Rosa rugosa were collected throughout 10 regions of Korea. Their genetic relationship was investigated by using morphological analysis and RAPD marker. The morphological analysis was measured for 7 quantitative and quantified for 4 qualitative traits. RAPD analysis obtained a total of 959 polymorphic bands by using twenty primers. Morphological analysis classified most according to the rose section system except for several species. The cluster analysis of genus Rosa based on RAPD data could identify the subgenus $Platyrhodon$ and $Eurosa$. The subgenus $Eurosa$ separated five sections; $Gallicanae$, $Cinnamomeae$, $Pimpinellifoliae$, $Synstylae$ and $Caninae$. Correlation analysis between morphological and RAPD analysis showed low significance ($r$ = 0.35). The accessions of R. rugosa belonged to the section $Cinnamomeae$ clustered into three groups at genetic distance ranging from 0.28 on the base of RAPD analysis. In conclusion, the genetic relationship of the genus Rosa was consistent to the previously reported rose section system, and domestic collections of $R.$ $rugosa$ were separated from 3 groups on the base of RAPD marker.

장미 속에 포함된 23종의 59계통을 수집하고, 해당화의 15계통은 국내의 10개 지역에서 수집하였다. 이 종들의 유전적 관계는 형태적 분석과 RAPD 마커를 이용하여 확인하였다. 형태적 분석은 7개의 양적 형질을 측정하고 4개의 질적 형질은 수치화하였다. RAPD 분석은 20개의 primer를 사용하여 총 959개의 다형성 밴드를 얻었다. 형태적 특성 분석은 전반적으로 종들이 section의 특성으로 분류되었으나, 부분적으로 몇 몇 종들은 분류에 어려움이 있었다. RAPD 결과를 바탕으로 장미 속의 군집분석을 수행한 결과, 아속 $Platyrhodon$$Eurosa$를 구분할 수 있었다. 그 중 아속 $Eurosa$는 5개의 section으로 분리되었다; $Gallicanae$, $Cinnamomeae$, $Pimpinellifoliae$, $Synstylae$ 그리고 $Caninae$. 형태적 분석과 RAPD 분석 간의 상관 관계는 유의성이 낮았다($r$=0.35). Sect. $Cinnmomeae$에 속하는 해당화는 RAPD 분석을 통해 유전적 거리 0.28에서 3그룹으로 군집을 형성하였다. 결과적으로, 장미 속의 유전적 관계는 이전에 보고된 rose section system과 일치하였으며, 국내에서 수집한 해당화는 RAPD 마커 분석에 의해 3그룹으로 구분되었다.

Keywords

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