The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Ethanol Extract of Cynanchum wilfordii Produces Endothelium-Dependent Relaxation in Rat Aorta and Anti-inflammatory Activity in Human Aortic Smooth Muscle Cells

  • Choi, Deok-Ho (College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee, Yun-Jung (College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kim, Eun-Joo (College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Li, Xiang (College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kim, Hye-Yoom (College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Hwang, Sun-Mi (College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Yoon, Jung-Joo (College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee, So-Min (College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Min, Eun-Kyeong (Oriental Internal Medicine, Joonghwa Medical Center) ;
  • Kang, Dae-Gill (College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee, Ho-Sub (College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University)
  • Received : 2010.10.04
  • Accepted : 2010.11.25
  • Published : 2010.11.30
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Abstract

Objective: The present study investigated the effect of ethanol extract of Cynanchum wilfordii (ECW) on vascular relaxation and vascular inflammation in rat artery isolated from rats and anti-inflammatory activity in human aortic smooth muscle cells (HASMC). Methods: Vascular tone and guanosine 3',5'-cyclic monophosphate (cGMP) production were examined in rat artery isolated from Sprague Dawley rats, in the presence of ECW. HASMC were incubated with tumor necrosis factor-alpha (TNF-${\alpha}$) or Angiotensin II for 24 h. Matrix metalloproteinase (MMP)-2 and anti-oxidant activity of ECW was investigated by pretreatment with ECW in HASMC. Results: Cumulative treatment of ECW relaxed aortic smooth muscles of rats in a dose-dependent manner. ECW-induced vasorelaxation was significantly decreased by pretreatment of L-arginine methyl ester (L-NAME) or oxadiazolo-quinoxalinone (ODQ). Furthermore, ECW treatment of thoracic aorta significantly increased cGMP production. Incubation of ECW with ODQ or L-NAME markedly decreased ECW-induced cGMP production. ECW treatment dose-dependently suppressed TNF-${\alpha}$- or Angiotensin II-induced increase in matrix metalloproteinase-2 expression in HASMC. Also, ECW exhibited 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity in vitro and reduced TNF-${\alpha}$-induced increase in reactive oxygen species production in a dose-dependent manner. Conclusions: Taken together, the results suggest that ECW exerts vascular relaxation via NO/cGMP signaling pathway and decreases MMP-2 expression via anti-oxidant activity.

Keywords

References

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