The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Selection of Beneficial Microbial Agents for Control of Fungal Diseases in the Phyllosphere of Cucumber Plant

오이 지상부의 주요 곰팡이 병해의 생물적 방제용 유용미생물의 선발

  • Lee, Sang-Yeob (Agricultural Microbiology Team, National Academy of Agricultural Science (NAAS), Rural Development Administration(RDA)) ;
  • Lee, Young-Kee (Crop Protection Division, National Academy of Agricultural Science (NAAS), Rural Development Administration(RDA)) ;
  • Park, Kyung-Seok (Agricultural Microbiology Team, National Academy of Agricultural Science (NAAS), Rural Development Administration(RDA)) ;
  • Kim, Yong-Ki (Organic Agriculture Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA))
  • 이상엽 (농촌진흥청 국립농업과학원 농업미생물팀) ;
  • 이영기 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 박경석 (농촌진흥청 국립농업과학원 농업미생물팀) ;
  • 김용기 (농촌진흥청 국립농업과학원 유기농업과)
  • Received : 2010.11.01
  • Accepted : 2010.12.09
  • Published : 2010.12.31
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Abstract

Bacillus subtilis B29, B. subtilis M10 and Streptomyces sp. CC19 obtained from phyllosphere of cucumber plants were selected for biological control of fungal air-borne diseases. For the downy mildew, diseased area of B. subtilis B29, B. subtilis M10 and Streptomyces sp. CC19 showed 0.5%, 20.2% and 42.0%, but that of control was 82.0% respectively, in cucumber seedling test. Incidence of powdery mildew by once application of B. subtilis B29, B. subtilis M10 and Streptomyces sp. CC19 was 2.8%, 3.6% and 12.3%, respectively, whereas that of control was 65.6%. On the gray mold, diseased area of B. subtilis B29, B. subtilis M10 and Streptomyces sp. CC19 was 8.0%, 30.8% and 5.2%, respectively, compared to 81.2% for the control. Therefore, B. subtilis B29 could be a prospective antagonist for biological control of powdery mildew, downy mildew and gray mold of cucumber plant.

오이 지상부에 발생하는 주요 곰팡이병인 노균병, 흰가루병 및 잿빛곰팡이병에 대한 생물적 방제를 위하여 오이식물제로부터 Bacillus subtilis B29, B. subtilis M10 and Streptomyces sp. CC19균주를 분리하였다. 오이 노균병에 대한 유묘검정에서 B. subtilis B29, B. subtilis M10과 Streptomyces sp. CC19균주는 0.5%, 20.2% 및 42.0%의 병반면적율을 나타내었지만, 무처리에서는 82.0% 발생하였다. 오이 흰가루병에 대한 비닐하우스 포장실험에서 B. subtilis B29, B. subtilis M10과 Streptomyces sp. CC19균주는 2.8%, 3.6%와 12.3% 발생하였지만 무처리에서 65.6% 발생면적을 나타내었다. 오이 잿빛곰팡이병에 대한 B. subtilis B29, B. subtilis M10과 Streptomyces sp. CC19균주는 8.0%, 30.8% 및 5.2%를 각각 나타내었고 무처리에서는 81.2%의 병반면적율을 나타내었다. 그러므로 B. subtilis B29균주는 오이에 발생하는 흰가루병, 노균병과 잿빛곰팡이병의 생물적 방제에 유망한 균주로 선발하였다.

Keywords

References

  1. Belanger, R. R., Dik, A. J. and Menzies. J. M. (1998) Powdery mildews recent Advances toward integrated control. In : Plant-microbe Interactions and Biological Control, ed. by Boland, G. J. and Kuykendall, D, L. pp. 89-126. Marcel dekker, New York, USA.
  2. Copping. L. G. (2004) The manual of biocontrol agents. The third edition of the biopesticide manual. BCPC. UK. 702pp.
  3. Fravel. D. R. (2005) Commercialization and implementation of biocontrol. Annu. Rev. Phytopathol. 43:337-359. https://doi.org/10.1146/annurev.phyto.43.032904.092924
  4. Jarvis, W. R. (1980) Epidemiology. In: The Biology of Botrytis (Ed. By J. R. Coley-Smmith, K. Verhoeff and W.R. Jarvis P.). Academic Press, London, pp. 219-250.
  5. 中澤靖彦, 黒沢美保子. 大塚範夫 (1994) キユウりべと病菌. 植物防疫 第 48卷 第 2号: 86-89.
  6. Schwinn, F. J. (1992) Significance of fungal pathogens in crop production. Pestic. Outlook1 3:18.
  7. Shlomo, B., Abraham, S., and Oded, Y. (2001) Isolation and characterization of a cold-tolerant strain of Fusarium proliferatum, a biocontrol agent of grape downy mildew. Phytopathology. 91(10):1062-1068. https://doi.org/10.1094/PHYTO.2001.91.11.1062
  8. Stuart, P. F., Roger, C. P., David, M. G., Robert, C. S., and Abraham, S. (1996) Fusarium proliferatum as a biocontrol agent against grape downy mildew. Phytopathology. 86(10): 1010-1017. https://doi.org/10.1094/Phyto-86-1010
  9. Cohen, Y. (1981) Downy mildew of cucurbits. pages 341-353. in : The downy mildew. Spencer. D. M.. Academic Press. New York.
  10. Yunis, H., Elad, Y. and Marher, Y. (1990) Effects of air temperature, relative humidity and canopy wetness on grey mould of cucumbers in unheated greenhouse. Phytoparasitca 18: 203-215. https://doi.org/10.1007/BF02980990
  11. Verhaar, M. A. and. Hijwegen, T. (1993) Efficient Production of phialoconidia of Verticillium lecanii for biocontrol of cucumber powdery mildew Sphaerotheca fuliginica. Neth. J. Path. 99: 101-103. https://doi.org/10.1007/BF01998478
  12. Wright, D. P., Scholes, D., Horton, p., Baldwin, B. C., and Shepphard, M. C. (1990) The relationship between the development of houstori of Erysiphe graminis and the energy status of leaves. pp. 223-226. In Current Reaserch in Photosynthesis, vol. 4. M. Baltscheffsky, ed. kluwer academic publishers, Dordrecht, Netherands.
  13. 농촌진흥청 (2000) 채소병해충 진단과 방제. 아카데미서적 331pp.
  14. 농촌진흥청 (2008) 목록공시 친환경 유기농자재 제품 자료집. 상록사 293pp.
  15. 이상엽, 이상범, 김용기 (2001) 시설채소 병해 종합관리를 위한 기반기술 개발연구. 593-605. 2000 작물보호연구. 농촌진흥청 농업과학기술원.
  16. 한국식물병리학회 (2009) 한국식물병명목록(제5판). 한국식물병리학회. 도서출판 제이와이 853pp.