The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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The Effect of Trans-cinnamaldehyde on the Gene Expression of Lipopolysaccharide-stimulated BV-2 Cells Using Microarray Analysis

Trans-Cinnamaldehyde가 Lipopolysaccharide로 처리된 BV-2 cell에 미치는 항염증 기전 연구: Microarray 분석

  • Sun, Young-Jae (Dept. of Applied Eastern Medicine, Grad. School) ;
  • Choi, Yeong-Gon (Dept. of Applied Eastern Medicine, Grad. School) ;
  • Jeong, Mi-Young (Dept. of Applied Eastern Medicine, Grad. School) ;
  • Hwang, Se-Hee (Dept. of Applied Eastern Medicine, Grad. School) ;
  • Lee, Je-Hyun (Dept. of Herbology, Col. of Eastern Medicine, Dongguk University) ;
  • Cho, Jung-Hee (Jeollanamdo Development Institute for Traditional Korean Medicine) ;
  • Lim, Sabina (Dept. of Applied Eastern Medicine, Grad. School)
  • 선영재 (경희대학교 대학원 한방응용의학과) ;
  • 최영곤 (경희대학교 대학원 한방응용의학과) ;
  • 정미영 (경희대학교 대학원 한방응용의학과) ;
  • 황세희 (경희대학교 대학원 한방응용의학과) ;
  • 이제현 (동국대학교 한의과대학 본초학교실) ;
  • 조정희 (전라남도한방산업진흥원) ;
  • 임사비나 (경희대학교 대학원 한방응용의학과)
  • Published : 2009.07.31
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Abstract

Objectives: Trans-cinnamaldehyde (TCA) is the main component of Cinnamomi Ramulus and it has been reported that TCA inhibits inflammatory responses in various cell types. Inflammation-mediated neurological disorders induce the activation of macrophages such as microglia in brain, and these activated macrophages release various inflammation-related molecules, which can be neurotoxic if overproduced. In this study, we evaluated gene expression profiles using gene chip microarrays in lipopolysaccharide (LPS)-stimulated BV-2 cells to investigate the antiinflammatory effect of TCA on inflammatory responses in brain microglia. Methods: A negative control group was cultured in normal medium and a positive control group was stimulated with $1{\mu}g/ml$ in the absence of TCA. TCA group was pretreated with $10{\mu}g/ml$ before $1{\mu}g/ml$ LPS stimulation. The oligonucleotide microarray analysis was performed to obtain the expression profiles of 28,853 genes using gene chip mouse gene 1.0 ST array in this study. Results: In positive control group, 1522 probe sets were up-regulated in the condition of the cutoff value of 1.5-fold change and 341 genes with Unigene ID were retrieved. In TCA group, 590 probe sets were down-regulated from among 1522 probe sets and 33 genes with Unigene ID were retrieved, which included 6 inflammation-related genes. We found out that Id3 gene is associated with transforming growth factor-${\beta}$ (TGF-${\beta}$) signaling pathway and Klra8 gene is related to natural killer cell-mediated cytotoxicity pathway. Conclusions: The results mean that TCA inhibits inflammatory responses through down-regulating the expressions of inflammation-related genes in LPS-stimulated BV-2 cells.

Keywords

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