Asian Journal of Turfgrass Science (아시안잔디학회지)

Turfgrass Society of Korea (한국잔디학회)
권호 표지

Embryogenic cell suspension culture and plant regeneration in zoysiagrass (Zoysia japonica Steud)

한국들잔디 배아세포의 부유배양과 식물체 재생

  • Fang, Wenjuan (Key Laboratory for Silviculture and Conservation of Ministry of Education, Institute of Turfgrass Science, Beijing Forestry University) ;
  • Han, Liebao (Key Laboratory for Silviculture and Conservation of Ministry of Education, Institute of Turfgrass Science, Beijing Forestry University) ;
  • Qi, Chunhui (Key Laboratory for Silviculture and Conservation of Ministry of Education, Institute of Turfgrass Science, Beijing Forestry University) ;
  • Li, Deying (Department of Plant Sciences, North Dakota State University) ;
  • Park, Tae-Yun
  • 팽왼주안 (베이징임업대학교 잔디연구소) ;
  • 한리바우 (베이징임업대학교 잔디연구소) ;
  • 퀴충휘 (베이징임업대학교 잔디연구소) ;
  • 리다이엥 (노스다코다대학교 식물학과) ;
  • 박태윤 (연세대학교 생명과학기술학부)
  • Received : 2009.08.15
  • Accepted : 2009.11.30
  • Published : 2009.12.31
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Abstract

Zoysiagrass (Zoysia japonica Steud) is a warm season turfgrass species widely used for sports field and golf courses. Many cultivars are propagated through vegetative methods. This study was conducted to develop an optimum culture medium and culture conditions for embryogenic callus induction and plant regeneration, and to establish a cell suspension culture system for use in zoysiagrass breeding and propagation. The results indicated that adding $Cu^{++}$ at 2.5 mg $L^{-1}$ to the induction medium was optimum for callus induction. Increasing the numbers of sub-culture cycles improved the quality of calli. The optimum dosage for cell suspension culture ranged from 2.5 to 10 mL. The embryogenic callus suspension used in this study had a plant regeneration rate of 58%.

한국들잔디는 운동경기장이나 골프 코스 등에 폭넓게 쓰이는 난지형 잔디이다. 많은 품종은 영양체로 번식한다. 이 연구는 배아세포 유도와 식물체 재생을 위한 적정 배양액 개발과 배양조건을 연구하여, 한국잔디의 육종과 번식을 위한 세포배양 체계 확보를 목적으로 하였다. 그 결과 $Cu^{++}\;2.5mgL^{-1}$를 유도 배양액에 첨가하는 것이 캘러스 유도를 위한 최적 농도 조건 이였음을 보여주었다. Sub-culture의 횟수의 증가는 캘러스의 질을 향상시켰다. 세포 부유배양을 위한 최적 용량은 2.5에서 10mL 사이였으며 본 연구에서 58%의 식물체 재생산비율을 보였다.

Keywords

References

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