Asian Journal of Turfgrass Science (아시안잔디학회지)

Turfgrass Society of Korea (한국잔디학회)
권호 표지

Inter-ramet Physiological Integration Detected in Buffalograss(Buchloe dactyloides (Nutt.) Engelm.) under Water Stress

수분스트레스 하에 있는 버팔로그래스에서 검출된 무성생식체의 생리학적 조정

  • Qian, Yongqiang (Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration) ;
  • Li, Deying (Department of Plant Sciences, North Dakota State University) ;
  • Han, Lei (Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration) ;
  • Ju, Guansheng (Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration) ;
  • Liu, Junxiang (Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration) ;
  • Wu, Juying (Beijing Research & Development Center for Grass and Environment, Beijing Academy of Agriculture and Forestry Science) ;
  • Sun, Zhenyuan (Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration)
  • 뀐용퀴앙 (베이징 주임업국 임학연구소) ;
  • 이다이엥 (노스다코다대학교 식물학과) ;
  • 한레이 (베이징 주임업국 임학연구소) ;
  • 주관쉥 (베이징 주임업국 임학연구소) ;
  • 류준샹 (베이징 주임업국 임학연구소) ;
  • 위주잉 (베이징 농.임학아카데미 초지연구개발센터) ;
  • 선젠얀 (베이징 주임업국 임학연구소)
  • Received : 2009.09.30
  • Accepted : 2009.11.30
  • Published : 2009.12.31
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Abstract

Buffalograss is an important turfgrass species with excellent cold, heat, and drought tolerance. Understanding the physiological integration of buffalograss under heterogeneous conditions helps to develop cultural practices that better use limited resources for uniform turf quality. The objective of this study was to evaluate physiological integration of buffalograss under water deficit stress and the involvement of lipid peroxidation and antioxidants in the process. In one experiment, buffalograss was planted in the center of a four-compartment growth unit. Watering frequencies, once a week(+) and once in two weeks(-), were combined with the sand(S) or peat(P) in each unit to generate five total treatments(P+S-P-S+, P+P+P+P+, S-S-S-S-, P-P-P-P-, and S+S+S+S+). The average number of shoot established from the heterogeneous root-zone medium was higher than the average of four possible homogeneous media. In second experiment, single ramet in Hoagland solution($S_0$) or single ramet in Hoagland solution with 20% PEG-6000($S_s$) were compared with two connectedramets under different treatments. Treatments for connected ramets were young ramet in Hoagland solution($Y_{os}$) and old ramet in Hoagland solution with 20% PEG-6000($O_{os}$), and old ramet in Hoagland solution($O_{ys}$) and young ramet in Hoagland solution with 20% PEG-6000($Y_{ys}$). Lipid peroxidation, antioxidants, and proline showedphysiological integration between ramets subjected to different levels of water stress. Superoxide dismutase(SOD), Guaiacol peroxidase(G-POD), malondialdehyde(MDA), and free proline also showed different time courses and relative activities during the physiological integration.

Buffalograss는 내한, 내서, 그리고 내한발에 우수한 주요 잔디 종 중 하나이다. 다양한 환경에서 buffalograss의 생리학적 조정(integration)을 이해하는 것은 균일한 잔디의 질을 도모하고 경종적 재배방법의 개발에 도움이 된다. 본 연구의 목적은 물 부족의 스트레스 처리에서의 buffalograss의 생리학적 조정과정에서 lipid peroxidation과 산화방지제의 연관성을 평가하였다. 한 실험에서 buffalograss는 네 개의 구분된 칸막이 성장 유닛의 중심에서 재배되었고, 일주일에 한번(+), 일주일에 두 번(-) 관수처리와 모래(S) 또는 피트(P)와를 혼합한 다섯 가지 토양 조합으로 처리하였다(P+S-P-S+, P+P+P+P+, S-S-S-S-, P-P-P-P-, and S+S+S+S+). 그 결과, 균일하게 혼합된 상토에서 생장한 줄기의 수가 네개의 단일 상토에 정착한 것보다 더 많았다. 두 번째 실험에서는 Hoagland 용액($S_o$), 또는 20% PEG-6000이 함유된 Hoagland용액($S_s$) 안에 하나의 라미트(무성생식체) 혹은 연결된 라마트를 다음과 같은 여러 가지 처리와 비교 실험하였다. 연결된 라미트들의 처리는 Hoagland 용액안의 어린 라미트($Y_{os}$)와 20%PEG-6000가 함유된 Hoagland 용액안의 성숙한 라미트($O_{os}$), Hoagland 용액 단독에 성숙한 라미트($O_{ys}$), 20%PEG-6000 함유된 Hoagland 용액안의 어린 라미트($Y_{ys}$)였다. Lipid peroxidation, antioxidants, proline은 각기 다른 수분 stress 정도에서 라미트들 간의 생리학적 활성을 보여 주었다. Superoxide dismutase (SOD), Guaiacol peroxidase (G-POD), malondi aldehyde (MDA), free proline의 활성도 처리 후 시간에 따라 상대적인 생리학적 활성을 보였다.

Keywords

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