Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
권호 표지
DOI QR코드

DOI QR Code

Growth Optimization of Delftia sp. for the Odor Control of Organic Waste

유기성 폐기물의 발생 악취 제거를 위한 Delftia sp.의 성장조건 최적화

  • Kwon, Hyuk-Ku (Intellectual Property Management Office, Industry-Academic Cooperation Foundation, Hoseo University) ;
  • Jung, Joon-Oh (Department of Environmental Engineering, Graduate School Hoseo University) ;
  • Chu, Duk-Sung (Department of Environmental Engineering, Graduate School Hoseo University) ;
  • Lee, Jang-Hoon (Department of Environmental Engineering, Graduate School Hoseo University)
  • 권혁구 (호서대학교 산학협력단 지식재산경영실) ;
  • 정준오 (호서대학교 환경공학과) ;
  • 추덕성 (호서대학교 환경공학과) ;
  • 이장훈 (호서대학교 환경공학과)
  • Received : 2009.08.24
  • Accepted : 2009.10.02
  • Published : 2009.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

We isolated and identified a microorganism which was excellent for ammonia oxidation in the biological control of ammonia gas in odor producing materials from organic composting. The isolated strain was tested for growth characteristics and ammonia elimination efficiency under various conditions of temperature, pH, carbon concentration and ammonia concentration. The strain was isolated from a culture broth used in a $NO_2$ producing test with Griess-Ilosvay reagent. The results of 16S rRNA sequence from the isolated strain by using BLANST (Basic Local Alignment Search Tool) and confirming RDP (Ribosomal Database Project II) and ERRD (The European Ribosomal RNA Database) indicate that the strain is related to Delftia sp. UV-Spectrophotometer (Shimadzu, UVmini-1240) was used as a microbial growth test by measuring turbidity on OD660nm and ammonia concentration was measured by Spectrophotometer (HACH, DR-4000). The optimum growth culture conditions of the ammonia oxidizer Delftia sp. were $30^{\circ}C$, pH 7, glucose concentration 1.00% and $(NH_4)_2SO_4$ 0.5 g/l. Ammonia elimination efficiency was over 94% under the same conditions.

Keywords

References

  1. Bae, J. S. : Characteristics of nitrous oxide(N2O) release from organic waste composting and population analysis of microorganism by FISH, Department of environmental education, The graduate school of education kyunghee university, 2003
  2. Ministry of environment republic of korea, Ministry for food agriculture forestry and fisheries : Management and use measure of Manure, 4 -6, 2004
  3. Ministry of environment republic of korea: Wastes generation and disposal in the country, 35-37, 2005
  4. Van Groenestijin, J. W., Hesselink, P. G. M. : Biotechniques for Air Pollution Control. Biodegradation, 4(4), 283-301, 1993 https://doi.org/10.1007/BF00695975
  5. Devinny, J. S., Deshusses, M. A., Webster, T. S. :Biofiltration for Air Pollution Control, Lewis Publishers, London, 1999
  6. Lee, J. O. : Characteristics of odor materials emitted during composting process of organic waste, Depertment of environmental engineering, The graduate school Hoseo university, 2006
  7. Yang, C. S., Kim, J. S. : Soil microbes experimental method, Worldscience, Seoul, 226-233, 2002
  8. HACH Company : DR-4000 procedure method 8038
  9. Liu, Z., Yang, H., Huang, Z., Zhou, P., Liu, S. J. :Degradation of aniline by newly isolated, extremely aniline-tolerant Delftia sp. AN3. Applied Micorbiology nd Biotechnology, 58(5), 679-628, 2002 https://doi.org/10.1007/s00253-002-0933-8
  10. Lee, W. S., Kim, J. E., Kim, H. S., Ahn, C. Y., Oh, H.M. : Serial degradation of perchloroethylene by Delftiasp. N6 after dechlorination using Fenton's reagent. Journal of Microbiology and Biotechnology, 16(11), 1734-1739, 2006
  11. Clinical and laboratory standard institute. Available from : http://www.nccls.org
  12. Kwon, H. K., Jung, J. O. : Isolation and characteristics of novel ammonia oxidizing bacteria Brevundimonas diminuta. Korean Journal of Environmental Health Society, 33(4), 293-298, 2007 https://doi.org/10.5668/JEHS.2007.33.4.293
  13. Jeong, G. T., Lee, G. Y., Lee, K. M., Lee, H. J., Ryu, H. W., Kim D., Chough, S. H., Kim, S. W., Cha, J.M., Jang, Y. S., Park, D. H. : Isolation and charaterization of odor tretment bacteria. Korean Journal of Biotechnology and Bioengineering, 20(5), 345-349, 2005