Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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Effects of Silver Nanoparticles Exposed in Somite Stage on Zebrafish Development

Somite stage에 노출된 은나노 입자가 zebrafish 발생에 미치는 영향

  • Published : 2009.03.31
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Abstract

Nanotechnology, one of the technologies that forms the core of the recent scientific innovation, is used much in our real lives. Especially products that use nano silver are being sold, with its positive characteristics resulting from the antibacterial effects of both nano materials and silver. But critiques have pointed out that nano silver diffused into everyday life too quickly as we do not have done any comprehensive research about the material, and worry that nano silver will affect the ecology adversely. Therefore, this research focuses on investigating the toxicity of silver nanoparticles first. To compare the effects of exposure to silver nanoparticles at pre-somite stage and somite stage(10 hours after fertilization), we exposed zebrafish embryos to silver nanoparticles(15, 30 ppt) during embryogenesis, and then checked the details of catalase enzyme activity. The hatch rate decreased in the silver nanoparticles exposed groups(15 and 30 ppt); furthermore, the hatched fishes had an abnormal notochord, damaged eyes and curved tail. The catalase activities of the 15 ppt exposed group at somite stage increased relative to those in the control group. Therefore, the silver nanoparticles could seriously damage the development of zebrafish embryos. Especially, exposure to silver nanoparticles at somite stage did severer damage than exposure since pre-somite stage did.

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References

  1. Asharani PV, Wu YL, Gong Z and Valiyaveettil S. Toxicity of silver nanoparticles inzebrafish models, Nanotechnology 2008; 19(25): 255102 https://doi.org/10.1088/0957-4484/19/25/255102
  2. Atli G, Alptekin O, Tükel S and Canli M. Response of catalase activity to $Ag^+,Cd^{2+},Cr^{6+},Cu^{2+}and Zn^{2+}$ in five tissues of freshwater fish Oreochromis niloticus, Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 2006; 143: 218-224 https://doi.org/10.1016/j.cbpc.2006.02.003
  3. Goth L. A simple method for determination of serum catalase activity and revision of reference range, Clin Chim Acta 1991; 196: 143-152 https://doi.org/10.1016/0009-8981(91)90067-M
  4. Hall TE and Johnston IA. Temperature and developmental plasiticity during embryogenesis in the Atlantic cod Gadus morhua, L Mar Biol 2003; 142: 833-840 https://doi.org/10.1007/s00227-003-1030-y
  5. Hwang ET, Lee JH, Chae YJ, Kim YS, Kim BC, Sang BI and Gu MB. Analysis of the Toxic Mode of Action of Silver Nanoparticles Using Stress-Specific Bioluminescent Bacteria, Small 2008; 4(6): 746-750
  6. Kimme W, Ballard S, Ullman BK and Schilling T. Stages of embryonic development in zebrafish, Developmental Dynamics 1995; 203: 253-310 https://doi.org/10.1002/aja.1002030302
  7. Moore MN. Do nanoparticles present ecotoxicological risks for the health of the aquatic environment?, Environment International 2006; 32: 967-976 https://doi.org/10.1016/j.envint.2006.06.014
  8. Park K. Toxicity of nanomaterials and strategy of risk assessment, J Environ Toxicol 2005; 20(4): 259-271
  9. Reijnders L. Cleaner nanotechnology and hazard reduction of manufactured nanoparticles, Journal of Cleaner Production 2006; 14: 124-133 https://doi.org/10.1016/j.jclepro.2005.03.018
  10. Stohs SJ and Bagchi D. Oxidative mechanisms in the toxicity of metal ions, Free Radical Biology & Medicine 1995; 18(2): 321-336 https://doi.org/10.1016/0891-5849(94)00159-H
  11. Westerfield M. The Zebrafish Book: A Guide for the Laboratory Use of Zebrafish, University of Oregon Press, Eugene, Oregon. 1995
  12. Yeo MK and Kang M. Effects of nanometer sized silver materials on biological toxicity during zebrafish embryogenesis, Bull Korean Chem Soc 2008; 29(6): 1179-1184 https://doi.org/10.5012/bkcs.2008.29.6.1179
  13. Yeo MK and Pak SW. Exposing zebrafish to silver nanoparticles during caudal fin regeneration disrupts caudal fin growth and p53 signaling, Mol & Cell Toxicol 2008; 4(4): 311-317