Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Optimization of an Extracellular Dextranase Production from Lipomyces starkeyi KCTC 17343 and Analysis of Its Dextran Hydrolysates

Lipomyces starkeyi KCTC 17343에 의한 extracellular dextranase 최적생산과 덱스트란 hydrolysates 분석

  • Chang, Yoon-Hyuck (Food Research Program, Agriculture and Agri-Food Canada) ;
  • Yeom, Joong-Hyun (Department of Oriental Medicine and Food Biotechnology, Joongbu University) ;
  • Jung, Kyung-Hwan (Department of Food and Biotechnology, Chungju University) ;
  • Chang, Byung-Chul (Department of Medical Genetic Engineering, Keimyung University School of Medicine and Institute for Medical Science) ;
  • Shin, Jung-Hee (Department of Food and Nutrition, Joongbu University) ;
  • Yoo, Sun-Kyun (Department of Oriental Medicine and Food Biotechnology, Joongbu University)
  • 장윤혁 (캐나다 Food Research program, Agriculture and Agri-Food) ;
  • 염중현 (중부대학교 한방건강식품학과) ;
  • 정경환 (충주대학교 식품생명공학과) ;
  • 장병철 (계명대학교 의학유전공학실) ;
  • 신정희 (중부대학교 식품영양학과) ;
  • 유선균 (중부대학교 한방건강식품학과)
  • Published : 2009.04.30
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Abstract

We optimized dextranase culture conditions by batch fermentation using Lipomyces starkeyi KCTC 17343. Furthermore, dextranase was purified by an ultra-membrane, and then dextran hydrolyzates were characterized. Cell growth and dextranase production varied depending on the initial culture pH and temperature. The conditions of optimal dextranase production were met in a pH range of 4-5 and temperature between $25-30^{\circ}C$. At optimal fermentation conditions, total enzyme activity and specific enzyme activity were about 4.85 IU/ml and 0.79 IU/g cells, respectively. The specific growth rate was examined to be $0.076\;hr^{-1}$. The production of dextranase in culture broth was very stably maintained after mid-log phase of growth. The enzyme hydrolyzed dextran into DP (degree of polymerization) 2 to 8 oligodextran series. Analysis of the composition of hydrolysates suggested that the enzyme produced is an endo-dextranase.

본 연구는 Lipomyces starkeyi KCTC 17343에 의한 dextranase 최적 생산 조건을 확립하고 dextran에 대한 효소 분해 특성을 규명하였다. 균주의 성장과 dextranase생산은 발효초기 pH와 온도에 따라 다르며 최적 pH는 4-5, 최적온도는 $25-30^{\circ}C$의 범위에서 결정이 되었다. 최적 발효조건에서의 dextranase 생산은 total enzyme activity가 4.85 IU/ml으로 나타났다. 이때의 발효균주의 specific growth rate는 $0.076h^{-1}$이었다. 발효 중 dextranase의 활성은 발효 정상기에서도 안정성을 유지하였다. Dextranase에 의한 dextran을 가수분해 결과, 가수분해물의 구성은 DP2 to 8에 이르는 올리고 덱스트란으로 이루어졌다.

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References

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