• Title, Summary, Keyword: dextran

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The physico-chemical nature of prepared dextran sulfates

  • Kim, Young-Choong
    • Archives of Pharmacal Research
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    • v.4 no.1
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    • pp.33-41
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    • 1981
  • The prepared dextran sulfates were characterized by measuring the reduced viscosisty at five different concentrations to obtain an intrinsic viscosity in both phosphate and tris buffers, pH 7.4, ionic strength of 0.1 Dextran sulfates having 0.81, 1.06 sulfate groups per hexose unit have reduced viscosity value below 40 ml/g whereas dextran sulfates having 1.21, 1.43, 1.69 sulfate groups per hexose unit have reduced viscosity value over 40 ml/g. Dextran sulfate having 1.21 sulfate groups per hexose unit had highest value of reduced viscosity. The reduced viscosity of dextran sulfate in tris buffer was always higher than that in phosphate buffer regardless of the sulfate content of dextran sulfate. The influence of the sulfation of the dextran sulfate. The influence of the sulfation of the dextran sulfate molecule on the dextran sulfate-LDL interaction was studied with three different dextran sulfate molecules. Dextran sulfate molecules having more than one sulfate group per hexose unit. The dextran sulfate having 0.81 sulfate groups per hexose unit showed considerably different precipitation curves in phosphate and tris buffers. This peculiar behavior of dextran sulfate having 0.81 sulfate groups per hexose unit in the two buffer systems was not noticed with dextran sulfate having more than one noticed with dextran sulfate having more than one sulfate group per hexose unit.

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Mechanism of Dextran Synthesis by Dextransucrase (Dextransucrase에 의한 Dextran 생성기작에 관한 연구)

  • 윤명희;구윤모
    • KSBB Journal
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    • v.9 no.1
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    • pp.1-7
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    • 1994
  • A qualitative study was made on the mechanism of dextran synthesis by dextransucrase. Enzymatic synthesis of dextran was experimentally studied with initial sucrose concentration from 50g/$\ell$ to 150g/$\ell$. The molecular weight distribution of synthesized dextran was measured by using on-line gel Permeation chromatographic system Sucrose was observed not to work as a primer within the range of concentration tested. At the initial sucrose concentration of 50g/$\ell$, dextran with molecular weight of medium range ($10^4-2{\times}10^6$) was synthesized due to the mass transfer limitation of sucrose. The amount of the dextran of medium range decreased with the initial sucrose concentration. Dextran was likely to be synthesized by radical chain polymerization mechanism since the dextran of medium range was not produced at higher sucrose concentrations.

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Characterization of Dextran Produced by L. mesenteroides ATCC 13146

  • Yoo, Sun-Kyun
    • 한국생물공학회:학술대회논문집
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    • pp.358-362
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    • 2005
  • High molecular weight dextran (39% alcohol, v/v), less soluble dextran, eluted from this column between T 500 and T 2000, a commercial linear dextran, Soluble dextran (45% alcohol, v/v) eluted at between T 70 and T 150 dextran. The molecular weight average of total dextran (50% alcohol, v/v) was between 150,000 to 500,000. A few oligosaccharides were detected from hydrolyzates of less soluble dextran. The hydrolyzates of soluble dextran were a family of DP 1 to 6 isomaltooligosaccharides. Compounds greater than DP 4 were branched isomaltooligosaacharides.

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Studies on the Fractional Precipitation of Dextran by Nonsolvent Addition Method (비용매 첨가법을 이용한 Dextran 분별침전에 관한 연구)

  • 최성우;구윤모
    • KSBB Journal
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    • v.10 no.3
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    • pp.257-263
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    • 1995
  • Fractional precipitation of "native" dextran by the method of nonsolvent addition was studied. Precipitational phenomenon of fractioned dextran was characterized with the quantitative determination of the precipitational capacity of nonsolvents with different dielectric constants. Based upon this characterization, the fractional precipitation process for the dextran production was developed. From the precipitational characteristics, a first-order relationship between the molecular weight of fractioned dextran and the content of nonsolvents was formulated. The slopes of the first-order equation were correlated with the dielectric constants of the nonslovents. A modified fractionation process was constructed on the basis of refractionation method and employed to increase the efficiency of the controlled production of dextran.

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In vitro Evaluation of Dextran-5-aminosalicylic Acid Conjugate as a Polymeric Colon-specific Prodrug of 5-aminosalicylic Acid

  • Jung, Yun-Jin;Jeon, Hyun-Chu;Choi, Dea-Kyu;Kim, Young-Mi
    • Journal of Pharmaceutical Investigation
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    • v.37 no.1
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    • pp.45-49
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    • 2007
  • Dextran-5-aminosalicylic acid conjugate (dextran-5-ASA) was in vitro-evaluated as a polymeric colon-spe-cific prodrug of 5-aminosalicylic acid (5-ASA). Chemical stability of dextran-5-ASA in the pH 1.2 or 6.8 buffer solutions was investigated at 37 for 6 hrs. The dextran backbone was not degraded and no 5-ASA release was detected. Moreover, dextran-5-ASA neither liberated 5-ASA in the homogenates of the small intestine of rats nor was transported across Caco-2 cell monolayers, suggesting no significant loss of dextran-5-ASA during transit through the upper intestine. Furthermore, incubation of dextran-5-ASA in 10% cecal contents of rats released about 37% and 55% of 5-ASA bound to dextran in 8 hr and 24 hr, respectively. While that with either esterase or dextranase failed to liberate 5-ASA from the polymeric prodrug, incubation of dextran-5-ASA with both esterases and dextranse released 5-ASA up to about 24% of 5-ASA bound to dextran. These results suggest that, after oral administration of dextran-5-ASA, the polymeric prodrug is delivered specifically to and releases 5-ASA in the large intestine, and reveal that the 5-ASA release by cleavage of the ester bond requires precedent depolymerization of the dextran backbone.

Effect of Reaction Time on the Rheological Properties of Dextran Formed Solution Produced by Crude Dextransucrase from Leuconostoc rnesenteroides Sikhae (Leuconostoc mesenteroides Sikhae의 Crude Dextransucrase 반응시간에 따른 Dextran 생성액의 유변성)

  • 박춘상;이철호
    • Microbiology and Biotechnology Letters
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    • v.20 no.3
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    • pp.316-323
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    • 1992
  • Studies on the changes in rheological properties, molecular weight distribution and dextran yield after being reacted in lO%(w/w) sucrose concentration were performed with crude dextransucrase produced from Leuconostoc mesenteroides isolated from Sikhae. The reaction rate of dextran formation was monitored by sugar analysis with HPLC and by the changes in apparent viscosity. According to the periodate oxidation test, the dextran produced in this experiment was estimated to have 89% $\alpha$-(1->6) main linkages and 11% $\alpha$-(1->) side linkages. The rheological properties of the dextran solution formed changed with reaction time, and it was related to the changes in molecular weight distribution of dextran as determined by GPC analysis. As the reaction proceeded, the rheological behavior changed from Newtonian to non-Newtonian, showing Binghampseudoplastic and thixothropic flow behavior. The apparent viscosity of dextran formed solution increased with increasing reaction time, reached a maximum value of 2680 cP ($\gamma$=$33.75s^{-1}$, $25^{\circ}C$) by enzyme reaction for 8 hours, and then decreased. The temperature dependency of dextran formed solutions was well expressed by the Arrhenius equation and the activation energy reached a maximum value of 1.69 kcal/mole by enzyme reaction for 8 hours.

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In situ viscoelastic properties of insoluble and porous polysaccharide biopolymer dextran produced by Leuconostoc mesenteroides using particle-tracking microrheology

  • Jeon, Min-Kyung;Kwon, Tae-Hyuk;Park, Jin-Sung;Shin, Jennifer H.
    • Geomechanics and Engineering
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    • v.12 no.5
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    • pp.849-862
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    • 2017
  • With growing interests in using bacterial biopolymers in geotechnical practices, identifying mechanical properties of soft gel-like biopolymers is important in predicting their efficacy in soil modification and treatment. As one of the promising candidates, dextran was found to be produced by Leuconostoc mesenteroides. The model bacteria utilize sucrose as working material and synthesize both soluble and insoluble dextran which forms a complex and inhomogeneous polymer network. However, the traditional rheometer has a limitation to capture in situ properties of inherently porous and inhomogeneous biopolymers. Therefore, we used the particle tracking microrheology to characterize the material properties of the dextran polymer. TEM images revealed a range of pore size mostly less than $20{\mu}m$, showing large pores > $2{\mu}m$ and small pores within the solid matrix whose sizes are less than $1{\mu}m$. Microrheology data showed two distinct regimes in the bacterial dextran, purely viscous pore region of soluble dextran and viscoelastic region of the solid part of insoluble dextran matrix. Diffusive beads represented the soluble dextran dissolved in an aqueous phase, of which viscosity was three times higher than the growth medium viscosity. The local properties of the insoluble dextran were extracted from the results of the minimally moving beads embedded in the dextran matrix or trapped in small pores. At high frequency (${\omega}>0.2Hz$), the insoluble dextran showed the elastic behavior with the storage modulus of ~0.1 Pa. As frequency decreased, the insoluble dextran matrix exhibited the viscoelastic behavior with the decreasing storage modulus in the range of ${\sim}0.1-10^{-3}Pa$ and the increasing loss modulus in the range of ${\sim}10^{-4}-1\;Pa$. The obtained results provide a compilation of frequency-dependent rheological or viscoelastic properties of soft gel-like porous biopolymers at the particular conditions where soil bacteria produce bacterial biopolymers in subsurface.

Formation of PEG/Dextran Aqueous Two-Phase System for Starch Hydrolysis Using $\alpha$-Amylase ($\alpha$-Amylase로 전분 가수분해를 위한 PEG/Dextran 수성 2상계 구성)

  • 박병춘;임동준
    • Microbiology and Biotechnology Letters
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    • v.20 no.2
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    • pp.190-195
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    • 1992
  • In the polyethylene glycol/dextran aqueous two-phase systems, volume ratio was increased and partition coefficient was decreased with the increase of potyethylene glycol molecular weight and concentration. However the volume ratio was decreased and the partition coefficient was increased with the increase of dextran molecular weight. On the other hand, the volume ratio and the partition coefficient were decreased with the increase of dextran concentration. Continuous enzymatic hydrolysis of soluble starch with $\alpha$-amylase which was produced by Bacillus amyloliquefaciens IF0 14141 was investigated in polyethylene glycol/dextran aqueous two-phase systems. Nonreacted soluble starch and $\alpha$-amylase were reused in these systems. $\alpha$-Amylase activity was maintained more than 100 hrs by recycling of $\alpha$-amylase from bottom of settler to reactor.

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Development of Washable Wool Using Environmental-friendly Spray UV-cure Finishing Technique - Using Photocrosslinkable Polymerr Dextran-methacrylate- (환경친화적 Spray UV-Cure 가공 기술을 이용한 물세탁 모직물(washable wool)의 개발 -광가교 고분자인 dextran-methacrylate를 이용하여-)

  • 김신희
    • Journal of the Korean Society of Clothing and Textiles
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    • v.28 no.11
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    • pp.1507-1515
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    • 2004
  • Washable wool was developed using environmental-friendly spray UV-cure technique. Photocrosslinkable polymer, dextran-methacrylate, was synthesized starting from natural biopolymer, dextran. The aqueous solution of dextran-methacrylate was applied to wool fabric with various concentrations to find out the optimum condition in minimizing felting shrinkage. The wool fabric subsequently cured by 365 nm UV, The effects of UV-cure time and photoinitiator concentration on felting behavior of wool were examined. As the concentration of dextran-methacrylate increased, the felting shrinkage decreased gradually. At concentration 0.5g/ml, the felting shrinkage of wool was negligible. Other properties such as air permeability, moisture content, wrinkle recovery, thickness and wettability were also evaluated. The surface coating of dextran polymer onto wool fiber was identified by SEM.

Use of Antibody Displayed Phage for the Detection of Dextran Using a Dipstick Assay and Transmission Electron Micrograph

  • Kim Du-Woon;Day Donal F.
    • Journal of Microbiology and Biotechnology
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    • v.16 no.8
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    • pp.1316-1319
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    • 2006
  • An antibody displayed phage collection (SBAE-2R), screened from a human synthetic phage antibody library (Fab 21ox), was used for the determination of dextran. The dextran-binding affinity was determined by serologically specific transmission electron microscopy (TEM) and a paper dipstick assay. The phage collection was distributed over the dextrancoated grids with 39$\pm$25 phages/$\mu$m$^2$ on the grids. Phages were not seen on dextran-coated grids exposed to the Fab 2lox phage library. The phage collection (SBAE-2R) produced 54$\pm$3 color normalized intensity (N.I.) from 125 ppm to 1,000 ppm of dextran and 5$\pm$1 (N.I.) for 63 ppm of dextran in a paper dipstick assay. This research extends the analytical options for dextran analysis by antibody displayed phage with a minimum of equipment usage.