Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Effects of Serotonin, Ecdysone and HCG on Ovarian Maturation of the Fleshy Prawn, Fenneropenaeus chinensis

대하(Fenneropenaeus chinensis)의 난소 성숙에 대한 Serotonin, Ecdysone 그리고 HCG의 효과

  • Published : 2009.12.31
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Abstract

The effects of serotonin (5-hydroxytryptamine, 5-HT), ecdysone (20-hydroxyecdysone, 20-E) and HCG (human chorionic gonadotropin) on the ovarian maturation of the fleshy shrimp, Fenneropenaeus chinensis were investigated. Adult female fleshy shrimps were injected with 5-HT ($5{\mu}g$/g body weight (BW) or $20{\mu}g$/g BW), 20-E ($5{\mu}g$/g BW, $10{\mu}g$/g BW or $20{\mu}g$/g BW) or HCG (5 IU/g BW or 10 IU/g BW) three times at 5-day intervals and were sacrificed 10 days after the last injection. The effects were compared against an intact control group and sham control group which received the injection of sterile saline solution as a vehicle. 20-E and HCG showed no effectiveness on ovarian maturation in fleshy shrimps. However flesh shrimp given 5-HT of $20{\mu}g$/g BW showed significant increase in gonadosomatic index (GSI) in early spring. The results suggest that treatment of 5-HT of $20{\mu}g$/g BW or more is useful approach for the nursery production of the fleshy shrimp, Fenneropenaeus chinensis aquaculture.

대하, Fenneropenaeus chinensis의 난소 성숙에 대한 serotonin(5-hydroxytryptamine, 5-HT), ecdysone(20-hydroxyecdysone, 20-E) 그리고 HCG(human chorionic gonadotropin)의 효과를 조사하였다. 대하에 5-HT(체중 g당 $5{\mu}g,\;20{\mu}g$), 20-E(체중g당 $5{\mu}g,\;10{\mu}g,\;20{\mu}g$) 그리고 HCG(체중 g당 5 IU, 10 IU)를 5일 간격으로 3회 주사한 후 10일이 더 지난 시점에 채집하여 성 성숙도지수(GSI)의 변화와 난소의 조직학적 변화를 조사하였다. 호르몬제의 효과를 비교하기 위해 멸균한 생리식염 수만을 주사한 대조구와 아무 것도 처리하지 않은 무처리구를 설정하였다. 20-E와 HCG를 처리한 실험구에서는 난소 성숙 효과가 전혀 나타나지 않았으나, 이른 봄에 5-HT를 체중 g당 $20{\mu}g$로 처리한 실험구에서는 유의한 난소 성숙효과를 보였다. 본 실험 결과에서 대하에 5-HT를 체중 g당 $20{\mu}g$ 이상 처리하면 난소성숙을 유도할 수 있다는 것을 보여주었으며, 이는 대하의 종묘 생산에 유용하게 사용될 수 있을 것이다.

Keywords

References

  1. Adiyodi R (1985) Reproductive and Control. In: The Biology of Crustaceaa, Vol. 9. Bills and Mantel LH (eds.), Academic Press, New York, pp 147-217.
  2. Anilkumar G, Adiyodi KG (1980) Ovarian growth induced by eyestalk ablation during the prebreeding season is not normal in the crab Paratelphusa hydrodromous. Int J Invert Reprod 2:95-105. https://doi.org/10.1080/01651269.1980.10553345
  3. Arechiga H, Banuelos E, Frixione E, Picones A, Rodriguez- Sosa L. 1990. Modulation of crayfish retinal sensitivity by 5-hydroxytryptamine. J Exp Biol 150:123-143.
  4. Beltz BS, Pontes M, Helluy SM, Kravitz EA (1990) Patterns of appearance of serotonin and proctolin immunoreactivities in the developing nervous system of the American lobster. J Neurobiol 21:521-542. https://doi.org/10.1002/neu.480210402
  5. Blanchet MF, Porcheron P, Dray F (1979) Variation du taux des ecdysteroid au cours des cycle de mue et de vitellogenes chez amphipode Orchestia gammarella. Int J Interbr Reprod 1:133-139. https://doi.org/10.1080/01651269.1979.10553307
  6. Blanchet-Tournier MF (1982) Quelque aspects des interacions hormonales entre la mue et la vitellogenese chez le crustace' Amphipode Orchesitia gammarellus (Pallas). Reprod Nutr De'velop 22:325-344. https://doi.org/10.1051/rnd:19820304
  7. Bomirski A, Klek-Kawinska E (1976) Stimulation of oogenesis in the sand shrimp, Crangon crangon, by a human gonadotropin. Gen Comp Endocrinol 30: 239-242. https://doi.org/10.1016/0016-6480(76)90074-5
  8. Buckmann D (1989) The significance of ecdysone in comparative physiology. In: Koolman J (Ed.), Ecdysone- From Chemistry to Mode of Action. Thieme, Stuttgart, pp 20-26.
  9. Chan SM (1995) Possible roles of 20-hydroxyecdysone in the control of ovary maturation in the white shrimp Penaeus vannamei (Crustacea: Decapoda). Comp Biochem Physiol 112C: 51-59.
  10. Di Cosmo A, Di Cristo C (1998) Neuropeptidergic control of the optic gland of Octopus vulgaris: FMRF-Amide and GnRH immunoreactivity. J Comp Neurol 398:1-12. https://doi.org/10.1002/(SICI)1096-9861(19980817)398:1<1::AID-CNE1>3.0.CO;2-5
  11. Di Cristo C, Paolucci M, Iglesias J, Sanchez J, Di Cosmo A (2002) Presence of two neuropeptides in the fusiform ganglion and reproductive ducts of Octopus vulgaris: FMRFamide and gonadotropin-releasing hormone (GnRH). J Exp Zool 292:267-276. https://doi.org/10.1002/jez.90000
  12. Di Fiore MM, Rastogi RK, Ceciliani F, Messi E, Botte V, Botte L, Pinelli C, D'Aniello B, D'Aniello A (2000) Mammalian and chicken I forms of gonadotropinreleasing hormone in the gonads of a protochordate, Ciona intestinalis. PNAS 97: 2343-2348. https://doi.org/10.1073/pnas.040549097
  13. Elofsson R (1983) 5-HT-like immunoreactivity in the central nervous system of the crayfish, Pacifastacus leniusculus. Cell Tissue Res 232: 221-236.
  14. Fingerman M, Nagabhushanam R, Sarojini R, Reddy PS (1994) Biogenic amines in crustaceans: identification, localization, and roles. J Crustacean Biol 14(3):413-437. https://doi.org/10.2307/1548990
  15. Fingerman M (1997a) Crustacean endocrinology: a retrospective, prospective, and introspective analysis. Physiol Zool 70(3):257-269.
  16. Fingerman M (1997b) Roles of neurotransmitters in regulating reproductive hormone release and gonadal maturation in decapod crustaceans. Invertebr Reprod Dev 31:47-54. https://doi.org/10.1080/07924259.1997.9672562
  17. Friesen KJ, Kaufman WR (2004) Effects of 20-hydroxyecdysone and other hormones on egg development, and identification of a vitellin binding protein in the ovary of the tick, Amblyomma hebraeum. J Insect Physiol 50:519- 529. https://doi.org/10.1016/j.jinsphys.2004.03.008
  18. Fernald RD, White RB (1999) Gonadotropin-releasing hormone genes: phylogeny, structure, and functions. Front Neuroendocrin 20:224-240. https://doi.org/10.1006/frne.1999.0181
  19. Gohar M, Souty C (1984) Action temporalle d'ecdysteroids sur la synthese proteique ovarianne in vitro chez le crustace isopode terrestre Porcellio dialatus (Brandt). Reprod Nutr Dev 24:137-145. https://doi.org/10.1051/rnd:19840205
  20. Goldberg JI, Garofalo R, Price CJ, Chang JP (1993) Presence and biological activity of a GnRH-like factor in the nervous system of Helisoma trivolvis. J Comp Neurol 336:571-582. https://doi.org/10.1002/cne.903360409
  21. Goodwin TW (1978) Ecdysteroids: A new generic term. Nature 272:122.
  22. Gorbman A, Sower SA (2003) Evolution of the role of GnRH in animal (Metazoan) biology. Gen Comp Endocrinol 134:207-213. https://doi.org/10.1016/j.ygcen.2003.09.018
  23. Hagedorn HH (1985) The role of ecdysteroids in reproduction. In: Kerkut, G.A., Gilbert, L.I. (Eds.), Comprehensive Insect Physiology, Biochemistry and Pharmacology, vol. 8. Pergamon, Oxford, pp. 205-262.
  24. Hagedorn HH (1994) The endocrinology of the adult female mosquito. Adv Dis Vect Res 10:109-148.
  25. Han CH (1988) Physiological studies on the reproductive cycle of a freshwater prawn, Macrobrachium nipponense (De Hann), Ph. D. Thesis, The Univ. of Tokyo, Tokyo, Japan.
  26. Han CH, Kim DJ (1993) Studies on the X-organ of eyestalk and the photoperiod for the control of gonadal maturation in a freshwater prawn, Macrobrachium nipponense (De Haan). Bull Korean Fish Soc 26(1):76-90.
  27. Herp FV, Payen GG (1991) Crustacean neuroendocrinology: perspectives for the control of reproduction in aquacultural systems. Bull Inst Zool Academia Sinica Monograph 16:513-539.
  28. Huber R, Smith K, Delago A, Isaksson K, Kravitz EA (1997) Serotonin and aggressive motivation in crustaceans: altering the decision to retreat. PNAS 94:5939-5942. https://doi.org/10.1073/pnas.94.11.5939
  29. Kravitz EA (2000) Serotonin and aggression insights gained from a lobster model system and speculations on the role of amine neurons in a complex behavior. J Comp Physiol, A Sens Neural Behav Physiol 186:221-238. https://doi.org/10.1007/s003590050423
  30. Iwakoshi-Ukena E, Ukena K, Takuwa-Kuroda K, Kanda A, Tsutsui K, Minakata H (2004) Expression and distribution of octopus gonadotropin-releasing hormone in the central nervous system and peripheral organs of the octopus (Octopus vulgaris) by in situ hybridization and immunohistochemistry. J Comp Neurol 477:310-323. https://doi.org/10.1002/cne.20260
  31. Kelemen J, Smith LR (1980). Induced ovarian devlopment and spawning of Penaeus indicus in a recirculating laboratory tank after uni-lateral eyestalk enucleation. Aquaculture 21:55-62. https://doi.org/10.1016/0044-8486(80)90125-8
  32. Kulkarni GK, Fingerman M (1992) Quantitative analysis by reverse phase high performance liquid chromatography of 5-hydroxytryptamine in the central nervous system of the red swamp crayfish, Procambarus clarkii. Biol Bull 182:341-347. https://doi.org/10.2307/1542254
  33. Kulkarni GK, Nagabhushanam R (1980) Role of ovary inhibiting hormone from eyestalks of marine panaeid prawns (Parapenaeopsis hardwickii) during ovarian development cycle. Auaculture 19:13-19.
  34. Lachaise F, Lafont R (1984) Ecdysteroid metabolism in a crab, Carcinus maenas L. Steroids 43:243-260. https://doi.org/10.1016/0039-128X(84)90043-6
  35. Lee JY (2005) The study on the reproductive physiology of the flesh prawn, Fenneropenaeus chinensis. Ph.D. thesis, Dongeui Univ, pp 24-42.
  36. Lee CY, Yau SM, Liau CS, Huang WJ (2000) Serotonergic regulation of blood glucose levels in the crayfish, Procambarus clarkii site of action and receptor characterization. J Exp Zool 286:596-605. https://doi.org/10.1002/(SICI)1097-010X(20000501)286:6<596::AID-JEZ6>3.0.CO;2-S
  37. Lee CY, Yang PF, Zou HS (2001) Serotonergic regulation of crustacean hyperglycemic hormone secretion in the crayfish Procambarus clarkii. Physiol Biochem Zool 74:376-382. https://doi.org/10.1086/320430
  38. Livingstone MS, Schaeffer SF, Kravitz EA (1981) Biochemistry and ultrastructure of serotonergic nerve endings in the lobster: serotonin and octopamine are contained in different nerve endings. J Neurobiol 12:27-54. https://doi.org/10.1002/neu.480120104
  39. Luschen WS, Willig A, Jaros PP (1993) The role of biogenic amines in the control of blood glucose level in the decapod crustacean, Carcinus maenas L. Comp Biochem Physiol C 105:291-296. https://doi.org/10.1016/0742-8413(93)90210-C
  40. Meeratana P, Withyachamnzrnkul B, Damrongphol P, Wongprasert K, Suseangtham A, Sobhon P (2006) Serotonin induces ovarian maturation in giant freshwater prawn broodstock, Macrobrachium rosenbergii de Man. Aquaculture 260: 315-325. https://doi.org/10.1016/j.aquaculture.2006.06.010
  41. Menasveta P, Piyatiratitivorakula S, Rungsupaa S, Moreea N, Fastb AW (1993) Gonadal maturation and reproductive performance of giant tiger prawn (Penaeus monodon Fabricius) from the Andaman Sea and pond-reared sources in Thailand. Aquaculture 116:191-198. https://doi.org/10.1016/0044-8486(93)90008-M
  42. Meusy JJ, Blanchet MF, Junera H (1977) Mue et vitellogenese chez le crustace' Amphipode Orchestia gammerella (Pallas). II. Etude de la synthese de la vitelloge'nie ('fraction prote'ique femelle' de l"hemolymph) apre's destruction des organes Y. Gen Comp Endocrinol 33: 35-40. https://doi.org/10.1016/0016-6480(77)90124-1
  43. Ngernsoungnern A, Ngernsoungnern P, Weerachatyanukul W, Chavadej J, Sobhon P, Sretarugsa P (2008) The existence of gonadotropin-releasing hormone (GnRH) immunoreactivity in the ovary and the effects of GnRHs on the ovarian maturation in the black tiger shrimp Penaeus monodon. Aquaculture 279:197-203. https://doi.org/10.1016/j.aquaculture.2008.04.018
  44. Nijhout HF (1994) Insect Hormones. Princeton University Press, Princeton NJ.
  45. Okumura T, Han CH, Suzuki Y, Aida K, Hanyu I (1992) Changes in hemolymph vitellogenin and ecdysteroid levels during the reproductive and nonreproductive molt cycles in the freshwater prawn Macrobrachium nipponense. Zool Sci 9:37-45.
  46. Panouse JB (1943) Influence de l'ablation de pedoncle oculaire sur la croissance de l'ovaire chez la crevette Leander serratus. C R Acad Sci Paris 217:535-555.
  47. Panouse JB (1944) L'action de la glande du sinus sur l'ovaire chez la crevette Leander. C R Acad Sci Paris 218:293-294.
  48. Pazos AJ, Mathieu M (1999) Effects of five natural gonadotropin-releasing hormones on cell suspensions of marine bivalve gonad: stimulation of gonial DNA synthesis. Gen Comp Endocrinol 113:112-120. https://doi.org/10.1006/gcen.1998.7186
  49. Rodriguez-Sosa L, Picones A, Rosete G, Arechiga YS (1997) Localization and release of 5-hydroxytryptamine in thee crayfish eyestalk. J Exp Biol 200:3067-3077.
  50. Santhoshi S, Sugumar V, Munuswamy N (2009) Serotonergic stimulation of ovarian maturation and hemolymph vitellogenin in the Indian white shrimp, Fenneropenaeus indicus. Aquaculture 291:192-199. https://doi.org/10.1016/j.aquaculture.2009.03.016
  51. Sarojini R, Nagabhushanam R, Fingerman M (1995) In vivo effects of dopamine and dopaminergic antagonists on testicular maturation in the red swamp crayfish, Procambarus clarkii. Biol Bull 187(3):340-346.
  52. Sherwood NM, Lovejoy DA, Coe IR (1993) Origin of mammalian gonadotropin-releasing hormones. Endocrin Rev 14: 241-254.
  53. Souty C, Besse G, Picaud JL (1982) Stimulation par l'ecdysone du taux hemolymph a tique de la vitellogenine chez le crustace isopode terrestre Porcellio dialatus (Brandt). C R Acad Sci Paris Ser III 294:1057-1059.
  54. Souty C, Picaud JL (1984) Effet de l'injection d'une gonadotropine humaine sur la synthese et la liberation de la vitellogenine par le tissu adipeux du Crustace isopode marin Idotea balthica basteri. Audouin Gen Comp Endocrinol 54(3):418-421. https://doi.org/10.1016/0016-6480(84)90156-4
  55. Steel CGH, Vafopoulou X (1998) Ecdysteroid titers in haemolymph and other tissues during molting and reproduction in the terrestrial isopod, Oniscus ascellus (L.). Invertebr Reprod Develop 34:187-194. https://doi.org/10.1080/07924259.1998.9652652
  56. Suzuki S, Yamasaki K, Fugita T, Mamiya Y, Sonobe H (1996) Ovarian and hemolymph ecdysteroids in the terrestrial isopod Armadillidium vulgare (Malacostracan Crustacea). Gen Comp Endocrinol 104:129-138. https://doi.org/10.1006/gcen.1996.0155
  57. Tsai PS (2006) Gonadotropin-releasing hormone in invertebrates: structure, function, and evolution. Gen Comp Endocrinol 148:48-53. https://doi.org/10.1016/j.ygcen.2005.09.016
  58. Tsai PS, Maldonado TA, Lunden JB (2003) Localization of gonadotropin-releasing hormone in the central nervous system and a peripheral chemosensory organ of Aplysia californica. Gen Comp Endocrinol 130:20-28. https://doi.org/10.1016/S0016-6480(02)00519-1
  59. Tsutsui H, Yamamoto N, Ito H, Oka Y (1998) GnRHimmunoreactive neuronal system in the presumptive ancestral Chordate Ciona intestinalis (Ascidian). Gen Comp Endocrinol 112:426-432. https://doi.org/10.1006/gcen.1998.7160
  60. Vaca AA, Alfaro J (2000) Ovarian maturation and spawning in the white shrimp, Penaeus vannamei, by serotonin injection. Aquaculture 182:373-385. https://doi.org/10.1016/S0044-8486(99)00267-7
  61. Vafopoulou X, Steel CGH (1995) Vitellogenesis in the terrestrial isopod, Oniscus asellus (L.): Characterization of vitellins and vitellogenins and changes in their synthesis throughout the intermolt cycle. Invertebr Reprod Dev 28:87-95. https://doi.org/10.1080/07924259.1995.9672469
  62. Wongprasert K, Asuvapongpatana A, Poltana P, Tiensuwan M, Withyachumnarnkul B (2006) Serotonin stimulates ovarian maturation and spawning in the black tiger shrimp Penaeus monodon. Aquaculture 261:1447-1454. https://doi.org/10.1016/j.aquaculture.2006.08.044
  63. Yano I (1984) Induction of rapid spawning in Kuruma prawn, Penaeus japonicus, through unilateral eyestalk enucleation. Aquaculture 40:265-268. https://doi.org/10.1016/0044-8486(84)90195-9
  64. Young KG, Chang JP, Goldberg JI (1999) Gonadotropinreleasing hormone neuronal system of the fresh water snails Helisoma trivolvis and Lymnaea stagnalis: possible involvement in reproduction. J Comp Neurol 404:427-437. https://doi.org/10.1002/(SICI)1096-9861(19990222)404:4<427::AID-CNE1>3.0.CO;2-R
  65. Zhang L, Wayne NL, Sherwood NM, Postigo HR, Tsai PS (2000) Biological and immunological characterization of multiple GnRH in an opisthobranch mollusk, Aplysia californica. Gen Comp Endocrinol 118:77-89. https://doi.org/10.1006/gcen.2000.7457
  66. Zhang L, Tello JA, Zhang W, Tsai PS (2008) Molecular cloning, expression pattern, and immunocytochemical localization of a gonadotropin-releasing hormone-like molecule in the gastropod mollusk, Aplysia californica. Gen Comp Endocrinol 156:201-209. https://doi.org/10.1016/j.ygcen.2007.11.015