The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지

Hexane Fraction of Zingiberis Rhizoma Crudus Extract Inhibits the Production of Nitric Oxide and Pro-inflammatory Cytokines in LPS-stimulated BV2 Microglial Cells

뇌신경소교세포(腦神經小膠細胞)에서 생강 헥산 분획물의 염증매개물질 생성(生成) 억제효과(抑制效果)

  • Jung, Hwan-Yong (Dept. of Oriental Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Joo, Ye-Jin (Dept. of Oriental Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Jung, Hye-Mi (Dept. of Oriental Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Shin, Woo-Jin (Samse Oriental Medical Hospital) ;
  • Seo, Un-Kyo (Dept. of Oriental Internal Medicine, College of Oriental Medicine, Dongguk University)
  • 정환용 (동국대학교 한의과대학 내과학교실) ;
  • 주예진 (동국대학교 한의과대학 내과학교실) ;
  • 정혜미 (동국대학교 한의과대학 내과학교실) ;
  • 신우진 (삼세한방병원 내과) ;
  • 서운교 (동국대학교 한의과대학 내과학교실)
  • Published : 2009.03.31
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Abstract

Objectives: The present study is focused on the inhibitory effect of the rhizome hexane fraction extract of Zingiberis Rhizoma Crudus (ginger hexan extract; GHE) on the production of inflammatory mediators such as NO, $PGE_2$, and proinflammatory cytokines in lipopolysaccharide (LPS)-stimulated BV2 cells, a mouse microglial cell line. Methods: We separated the hexane fraction from Zingiberis Rhizoma Crudus's methanol extract. The inhibitory and anti-inflammatory effect of GHE was examined on microglial activation. Results: GHE significantly inhibited the excessive production of NO, $PGE_2$, TNF-${\alpha}$, and IL-1${\beta}$ in LPS-stimulated BV2 cells. In addition, GHE attenuated the mRNA expressions and protein levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and proinflammatory cytokines. Conclusion: The anti-inflammatory properties of GHE may make it useful as a therapeutic candidate for the treatment of human neurodegenerative diseases.

Keywords

References

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