The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Identification of a Pathogen-Induced Glycine max Transcription Factor GmWRKY1

  • Kang, Sang-Gu (Department of Biotechnology, School of Biotechnology, Yeungnam University) ;
  • Park, Eui-Ho (Department of Biotechnology, School of Biotechnology, Yeungnam University) ;
  • Do, Kum-Sook (Department of Biotechnology, School of Biotechnology, Yeungnam University)
  • Published : 2009.12.01
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Abstract

On screening pathogen-resistant soybean, we identified a WRKY type transcription factor named a Glycine max WRKY1 (GmWRKY1). Expression of GmWRKY1 gene was induced in the soybean sprout by Pseudomonas infection. The GmWRKY1 was expressed in all of the tissues with high levels in stems, leaves and developing seeds. The protein Gm WRKY1 contains highly conserved two WRKY DNA-binding domains having two $C_2-H_2$ zinc-finger motif ($C-X_{4-5}-C-X_{22-23}-H-X-H$) in its N-terminal and C-terminal amino acid sequences. In electrophoresis mobility shift assay, the GmWRKY1 protein bound specifically to W-box elements in the promoters of defense related genes. These results demonstrated that GmWRKY1 is one of the soybean WRKY family genes and the plant-specific transcription factors for defense processes.

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References

  1. AbuQamar, S., Chen X., Dhawan, R., Bluhm, B., Salmeron, J., Lam, S., Dietrich, R. A. and Mengiste, T. 2006. Expression profiling and mutant analysis reveals complex regulatory networks involved in Arabidopsis response to Botrytis infection. Plant J. 48:28-44 https://doi.org/10.1111/j.1365-313X.2006.02849.x
  2. Asai, T., Tena, G., Plotnikova, J., Willmann, M. R., Chiu, W. L., Gomez-Gomez, L., Boller, T., Ausubel, F. M. and Sheen, J. 2002. MAP kinase signalling cascade in Arabidopsis innate immunity. Nature 415:977-983 https://doi.org/10.1038/415977a
  3. Bostock, R. M. 2005. Signal crosstalk and induced resistance: straddling the line between cost and benefit. Annu. Rev. Phytopathol. 43:545-580 https://doi.org/10.1146/annurev.phyto.41.052002.095505
  4. Chen, C. and Chen, Z. 2000. Isolation and characterization of two pathogen- and salicylic acid-induced genes encoding WRKY DNA-binding proteins from tobacco. Plant Mol. Biol. 42:387-396 https://doi.org/10.1023/A:1006399311615
  5. Chen, C. and Chen, Z. 2002. Potentiation of developmentally regulated plant defense response by AtWRKY18, a pathogen-induced Arabidopsis transcription factor. Plant Physiol. 129:706-716 https://doi.org/10.1104/pp.001057
  6. Ciolkowski, I., Wanke, D., Birkenbihl, R. P. and Somssich, I. E. 2008. Studies on DNA-binding selectivity of WRKY transcription factors lend structural clues into WRKY-domain function. Plant Mol. Biol. 68:81-92 https://doi.org/10.1007/s11103-008-9353-1
  7. Cormack, R. S., Eulgem, T., Rushton, P. J., Kochner, P., Hahlbrock, K. and Somssich, I. E. 2002. Leucine zipper-containing WRKY proteins widen the spectrum of immediate early elicitor-induced WRKY transcription factors in parsley. Biochim. Biophys. Acta. 1576:92-100 https://doi.org/10.1016/S0167-4781(02)00298-1
  8. Deslandes, L., Olivier, J., Theulieres, F., Hirsch, J., Feng, D. X., Bittner-Eddy, P., Beynon, J. and Marco, Y. 2002. Resistance to Ralstonia solanacearum in Arabidopsis thaliana is conferred by the recessive RRS1-R gene, a member of a novel family of resistance genes. Proc. Natl. Acad Sci. USA 99:2404-2409 https://doi.org/10.1073/pnas.032485099
  9. Dong, J. X., Chen, C. H. and Chen, Z. X. 2003. Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response. Plant Mol. Biol. 51:21-37 https://doi.org/10.1023/A:1020780022549
  10. Du, L. and Chen, Z. 2000. Identification of genes encoding receptor-like protein kinases as possible targets of pathogen- and salicylic acid-induced WRKY DNA-binding proteins in Arabidopsis. Plant J. 24:837-847 https://doi.org/10.1046/j.1365-313x.2000.00923.x
  11. Eulgem, T. and Somssich, I. E. 2007. Networks of WRKY transcription factors in defense signaling. Curr. Opin. Plant Biol. 10:366-371 https://doi.org/10.1016/j.pbi.2007.04.020
  12. Eulgem, T., Rushton, P. J., Robatzek, S. and Somssich, I. E. 2000. The WRKY superfamily of plant transcription factors. Trends in Plant Sci. 5:199-206 https://doi.org/10.1016/S1360-1385(00)01600-9
  13. Eulgem, T, Rushton, P. J., Schmelzer, E., Hahlbrock, K. and Somssich, I. E. 1999. Early nuclear events in plant defense signaling: rapid gene activation by WRKY transcription factors. EMBO J. 18:4689-4699 https://doi.org/10.1093/emboj/18.17.4689
  14. Jones, J. D. and Dangl, J. L. 2006. The plant immune system. Nature. 444:323-329 https://doi.org/10.1038/nature05286
  15. Journot-Catalino, N., Somssich, I. E., Roby, D. and Kroj, T. 2006. The transcription factors WRKY11 and WRKY17 act as negative regulators of basal resistance in Arabidopsis thaliana. Plant Cell 18:3289-3302 https://doi.org/10.1105/tpc.106.044149
  16. Kang, S. G, Cha, H. W., Chang, M. U. and Park, E. 2003. ldentification of differentially displayed genes of a Pseudomonas resistant soybean (Glycine max). Plant Pathol. J. 19:239-247 https://doi.org/10.5423/PPJ.2003.19.5.239
  17. Katagiri, F. 2004. A global view of defense gene expression regulation-a highly interconnected signaling network. Curr. Opin. Plant BioI. 7:506-511 https://doi.org/10.1016/j.pbi.2004.07.013
  18. Kim, K. C., Fan, B. and Chen, Z. 2006. Pathogen-induced Arabidopsis WRKY7 is a transcriptional repressor and enhances plant susceptibility to Pseudomonas syringae. Plant Physiology 142:1180-1192 https://doi.org/10.1104/pp.106.082487
  19. Li, J., Brader, G. and Palva, E. T. 2004. The WRKY70 transcription factor: a node of convergence for jasmonate-mediated and salicylate-mediated signals in plant defense. Plant Cell 6:319-331
  20. Li, J., Brader, G. Kariola, T. and PaIva, E. T. 2006. WRKY70 modulates the selection of signaling pathways in plant defense. Plant J. 46:477-491 https://doi.org/10.1111/j.1365-313X.2006.02712.x
  21. Lim, J. S., Do, K. S., Lee, D. S., Kang, S. G, Suh, S. G. and Park, E. 2008. Identifications of a sprout-rot pathogen Pseudomonas species SN239 and selection resistant soybean line. J. of Life Sci. 18:1771-1774 https://doi.org/10.5352/JLS.2008.18.12.1771
  22. Maeo, K., Hayashi, S., Kojima-Suzuki, H., Morikami, A. and Nakamura, K. 2001. Role of conserved residues of the WRKY domain in the DNA-binding of tobacco WRKY family proteins. Biosci. Biotechnol. Biochem. 65:2428-36 https://doi.org/10.1271/bbb.65.2428
  23. Oh, S.-K., Baek, K.-H., Park, J. M., Yi, S. Y., Yu, S. H., Kamoun, S. and Choi, D. 2008. Capsicum annuum WRKY protein CaWRKY1 is a negative regulator of pathogen defense. New Phytologist. 177:977-987 https://doi.org/10.1111/j.1469-8137.2007.02310.x
  24. Robatzek, S. and Somssich, I. E. 2001. A new member of the Arabidopsis WRKY transcription factor family, AtWRKY6, is associated with both senescence- and defence-related processes. Plant J. 28:123-33 https://doi.org/10.1046/j.1365-313X.2001.01131.x
  25. Rushton, P. J., Torres, J. T., Parniske, M., Wernert, P., Hahlbrock, K. and Somssich, I. E. 1996. Interaction of elicitor-induced DNA binding proteins with elicitor response elements in the promoters of parsley PR1 genes. EMBO J. 15:5690-5700
  26. Ulker, B. and Somssich, I. E. 2004. WRKY transcription factors: from DNA binding towards biological function. Curr. Opin. Plant Biol. 7:491-498 https://doi.org/10.1016/j.pbi.2004.07.012
  27. Wang, D., Weaver N. D., Kesawani, M. and Dong, X. 2005. Induction of protein secretory pathway is required for systemic acquired resistance. Science 308:1036-1040 https://doi.org/10.1126/science.1108791
  28. Xu, X. P., Chen, C. H., Fan, B. F. and Chen, Z. X. 2006. Physical and functional interactions between pathogen-induced Arabidopsis WRKY18, WRKY40, and WRKY60 transcription factors. Plant Cell 18:1310-1326 https://doi.org/10.1105/tpc.105.037523
  29. Yang, B., Srivastava, S., Deyholos, M. K. and Kav, N. N. V. 2007. Transcriptional profiling of canola (Brassica napus L.) responses to the fungal pathogen Sclerotinia sclerotiorum. Plant Sci. 173:156-171 https://doi.org/10.1016/j.plantsci.2007.04.012
  30. Yang, P., Wang, Z., Fan, B., Chen, C. and Chen, Z. 1999. A pathogen-and salicylic acid-induced WRKY DNA-binding activity recognizes the elicitor response element of the tobacco class I chitinase gene promoter. Plant J. 18:141-149 https://doi.org/10.1046/j.1365-313X.1999.00437.x
  31. Yu, D., Chen, C. and Chen, Z. 2001. Evidence for an important role of WRKY DNA binding proteins in the regulation of NPRI gene expression. Plant Cell 13:1527-1540 https://doi.org/10.1105/tpc.13.7.1527

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