Functional Activity of Water and Ethanol Extracts from Red Pepper (Capsicum annuum L.) Seeds

고추씨의 물과 에탄올 추출물의 생리활성

This study was carried out to investigate the extract yield, total polyphenolic compounds content, electron donating activity (EDA) and nitrite scavenging activity (NSA) of various red pepper (Capsicum annuum L.) seeds. The water and ethanol extracts showed yields in the ranges of $11.30{\sim}18.93%$ and $3.00{\sim}5.25%$, respectively. Ethanol extract yield was higher than water. In the total polyphenol content, water and ethanol extracts were in the range of $10.22{\pm}1.29{\sim}25.98{\pm}0.55mg/$ and $8.00{\pm}0.57{\sim}33.99{\pm}0.09mg/g$, respectively. Also, nitrite scavenging activities were $70.11{\pm}0.71{\sim}94.07{\pm}0.86%$ and $81.93{\pm}1.77{\sim}99.90{\pm}0.70%$ for water and ethanol extracts, respectively. In the electron donating activity, water extracts showed lower activity than ethanol extracts. The electron donating activity of water extracts was in the range of $0.04{\pm}0.07{\sim}14.31{\pm}0.06%$ while that of NSA of ethanol extracts was $35.48{\pm}0.23{\sim}73.83{\pm}0.04%$.

품종별 고추씨의 추출 수율은 물 추출물 $11.30{\sim}18.93%$, 에탄올 추출물 $3.00{\sim}5.25%$로 에탄올 추출보다는 물 추출물이 더 높은 수율을 보였다. 품종별 고추씨의 총 폴리페놀 함량을 조사한 결과, 물 추출물은 $10.22{\pm}1.29{\sim}25.98{\pm}0.55mg/g$의 범위인 반면 에탄올 추출물은 $8.00{\pm}0.57{\sim}33.99{\pm}0.09mg/g$이었다. 또 품종별 고추씨 용매 추출물의 아질산염 소거 작용을 측정한 결과 물 추출물은 $70.11{\pm}0.71{\sim}94.07{\pm}0.86%$, 에탄올 추출물은 $81.93{\pm}1.77{\sim}99.90{\pm}0.70%$의 높은 소거능을 보였고, 전자공여능의 경우 물 추출물은 $0.04{\pm}0.07{\sim}14.31{\pm}0.06%$로 전자공여능이 낮았으나, 에탄올 추출물은 $35.48{\pm}0.23{\sim}73.83{\pm}0.04%$의 전자공여능을 보였다.