Development and Reproduction (한국발생생물학회지:발생과생식)
- Volume 12 Issue 1
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- Pages.77-86
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- 2008
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- 2465-9525(pISSN)
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- 2465-9541(eISSN)
Derivation of Mouse ES Cells from Isolated Blastomeres in Culture Media Supplemented with LIF
LIF를 첨가한 배양액을 이용한 할구 유래 생쥐 배아줄기세포주의 확립
- Cho, Jae-Won (Laboratory of Reproductive Biology & Infertility, Cheil General Hospital & Women's Healthcare Center, Kwandong University of Medicine) ;
- Lim, Chun-Kyu (Laboratory of Reproductive Biology & Infertility, Cheil General Hospital & Women's Healthcare Center, Kwandong University of Medicine) ;
- Ko, Duck-Sung (Laboratory of Reproductive Biology & Infertility, Cheil General Hospital & Women's Healthcare Center, Kwandong University of Medicine) ;
- Kang, Hee-Jung (Laboratory of Reproductive Biology & Infertility, Cheil General Hospital & Women's Healthcare Center, Kwandong University of Medicine) ;
- Jun, Jin-Hyun (Laboratory of Reproductive Biology & Infertility, Cheil General Hospital & Women's Healthcare Center, Kwandong University of Medicine)
- 조재원 (관동대학교 의과대학 제일병원 생식생물학 및 불임연구실) ;
- 임천규 (관동대학교 의과대학 제일병원 생식생물학 및 불임연구실) ;
- 고덕성 (관동대학교 의과대학 제일병원 생식생물학 및 불임연구실) ;
- 강희정 (관동대학교 의과대학 제일병원 생식생물학 및 불임연구실) ;
- 전진현 (관동대학교 의과대학 제일병원 생식생물학 및 불임연구실)
- Published : 2008.06.30
Abstract
This study was carried out to investigate the effect of leukemia inhibitory factor (LIF) on the derivation of mouse ES cells from isolated blastomeres. Two-cell stage mouse embryos were obtained from superovulated BDF1 female mice. Collected embryos were cultured to blastocyst stage in culture medium supplemented with 0, 1,000, 2,500 or 5,000 U/mL of LIF. Cultured blastocysts were examined by counting the number of cells in the inner cell mass (ICM) and trophectoderm (TE) using differential staining method. When 2-cell embryos were cultured with 2,500 U/ml of LIF, the cell numbers of ICM significantly increased in comparing with those of the control(
본 연구에서는 LIF의 첨가가 분리된 할구 유래의 생쥐 배아줄기세포 확립에 미치는 영향을 살펴보았다. 과배란 유도된 BDF1 생쥐로부터 2-세포기의 배아를 회수하여 각기 LIF가 첨가되지 않은 배양액과 1,000, 2,500, 5,000 U/mL의 LIF가 첨가된 배양액에서 포배기 배아까지 배양하였다. 배양된 포배기 배아는 차별화 염색 방법을 이용하여 내세포괴와 영양외배엽의 수를 계수하였다. 2,500 U/mL의 LIF 첨가 시 대조군(