Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Optimization of the Process for Biodiesel Production Using a Mixture of Immobilized Rhizopus oryzae and Candida rugosa Lipases

  • Lee, Jong-Ho (Department of Chemical and Biological Engineering, Korea University) ;
  • Lee, Dong-Hwan (Department of Chemical and Biological Engineering, Korea University) ;
  • Lim, Jung-Soo (Digital Appliances R&D Team, Samsung Electronics Co. Ltd.) ;
  • Um, Byung-Hwan (Department of Chemical and Biological Engineering, University of Maine) ;
  • Park, Chul-Hwan (Department of Chemical Engineering, Kwangwoon University) ;
  • Kang, Seong-Woo (Department of Chemical and Biological Engineering, Korea University) ;
  • Kim, Seung-Wook (Department of Chemical and Biological Engineering, Korea University)
  • Published : 2008.12.31
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Abstract

In this study, the enzymatic process for biodiesel production was optimized using a mixture of immobilized Rhizopus oryzae and Candida rugosa lipases. The optimal temperature and agitation speed for biodiesel production were $45^{\circ}C$ and 300 rpm, respectively. The optimal ratio of R. oryzae and C. rugosa lipases in the mixture was 3:1 (w:w). When 3 mmol of methanol was the initial reaction medium and 3 mmol of methanol was added every 1.5 h during biodiesel production, biodiesel conversion was over 98% at 4 h. In addition, when the immobilized lipase mixture was reused, biodiesel conversion exceeded 80% after 5 reuses.

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References

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