The Korea Journal of Herbology (대한본초학회지)
- Volume 22 Issue 2
- /
- Pages.155-161
- /
- 2007
- /
- 1229-1765(pISSN)
- /
- 2288-7199(eISSN)
The effect of Ginkgo biloba Extract (GB) on Glucose Uptake in L6 Rat Skeletal Muscle Cells
L6 근육세포에서 은행잎 추출물의 당 흡수효과
- Kim, Soo-Cheol (Kohwang Medical Research Institute, College of Medicine, Kyung Hee University) ;
-
Han, Mi-Young
(Kohwang Medical Research Institute, College of Medicine, Kyung Hee University) ;
- Kim, Hak-Jae (Department of Pharmacology, College of Medicine, Kyung Hee University) ;
- Jung, Kyung-Hee (Kohwang Medical Research Institute, College of Medicine, Kyung Hee University)
- Published : 2007.06.30
Abstract
Objectives: Evidences suggests that Ginkgo biloba, a widely used traditional medicine, shows a hypoglycemic effect. Thus, we investigatd the effect of G. biloba extract (GB) on glucose uptake in L6 rat skeletal muscle cells. Method : Effect of GB on glucose uptake and phosphatidylinositol (PI) 3-kinase activity were assessed using Glucose uptake assay and PI 3-kinase assay, respectively. Also, AMP-activated protein kinase (AMPK), p38 mitogen activated protein kinase (p38 MAPK) expression were identified by Western blot. Results : Glucose uptake assay revealed that GB increased glucose uptake about 2.5-fold compared to thecontrol. GB stimulated the activity of PI 3-kinase which is a major switch element on the glucose uptake pathway. About a 6.5-fold increase in activity of PI 3-kinase was observed with GB. We then assessed the activity of AMPK, another regulatory molecule on the glucose uptake pathway. The result was that GB increased the phosphorylation level of both AMPK
Keywords